Human adenovirus type 2 protein IIIa I. Purification and characterization

Lemay, Pierre; Boudin, Marie-Laure; Milleville, Michèle; Boulanger, Pierre

doi:10.1016/0042-6822(80)90490-0

Cited by 37 publications

(19 citation statements)

References 31 publications

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“…Virus was purified on CsCl gradients and particle counts determined using the equation 1 mg/ml virus protein = 3.45 × 10 12 particles/ml. 28 Infectivity was measured by plaque assay in 293 cells; particle:infectivity ratios of 10-50 were obtained for all virus stocks.…”

Section: Methodsmentioning

confidence: 99%

Polymer-coated adenovirus permits efficient retargeting and evades neutralising antibodies

et al. 2001

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Adenovirus is a widely used vector for cancer gene therapy because of its high infection efficiency and capacity for transgene expression in both dividing and nondividing cells. However, neutralisation of adenovirus by pre-existing antibodies can lead to inefficient delivery, and the wide tissue distribution of the coxsackie and adenovirus receptor (CAR, the primary receptor for adenovirus type 5) precludes target selectivity. These limitations have largely restricted therapeutic use of adenovirus to local or direct administration. A successful viral gene therapy vector would be protected from neutralising antibodies and exhibit a preferential tropism for target cells. We report here the development of a covalent coating and retargeting strategy using a multivalent hydrophilic polymer based on poly-[N-(2-hydroxypropyl)metha-

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Section: Methodsmentioning

confidence: 99%

Polymer-coated adenovirus permits efficient retargeting and evades neutralising antibodies

et al. 2001

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“…A cloned version of the novel adenovirus genome was identified, the viral genome was released from the plasmid by restriction enzyme digestion, and virus replication was initiated by transfecting the genome into the E1-complementing 293 cells (24) using a polyethylenimine transfection method (23). Virus was amplified and purified from cell lysates by using CsCl density gradient centrifugation as described (25); virus was quantified from protein content (25) by using the conversion factor 1 mg/ml pure virion protein ϭ 3.4 ϫ 10 12 viral particles/ml (26). Control adenovirus AdJ5 is an Ad5 vector with the same viral genotype (E1/E3 defective) but lacking an expression cassette.…”

Section: Methodsmentioning

confidence: 99%

RICK Activates a NF-κB-dependent Anti-human Cytomegalovirus Response

Eickhoff¹,

Hanke²,

Stein-Gerlach³

et al. 2004

Journal of Biological Chemistry

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“…Quantification of viruses was based on protein content, 29 using the conversion 1 mg of viral protein ¼ 3.4 Â 10 12 viral particles (vp). 30 Typical vector stock titers were 10 12 vp/ml with a 1:100 infectious plaque forming unit to vector particle ratio.…”

Section: Adenoviral Vector Expressing Recombinant Human Lect2mentioning

confidence: 99%

The tumor suppressor function of LECT2 in human hepatocellular carcinoma makes it a potential therapeutic target

Ong¹,

Tan²,

Wang³

et al. 2011

Cancer Gene Ther

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Vascular invasion is one of the clinicopathologic features that are associated with early recurrence of human hepatocellular carcinoma (HCC). In this study, we have employed high-density Affymetrix oligonucleotide GeneChips (Affymetrix, Santa Clara, CA) to compare the expression profiles of HCC with and without vascular invasion. Data mining of the gene expression database established revealed that leukocyte cell-derived chemotaxin-2 (LECT2) transcripts were downregulated in HCC patients with vascular invasion. Expression of LECT2 in human HCC biopsies was significantly reduced (Po0.0001, fold change ¼ À7.2) when compared with non-tumorous adjacent liver tissues. The reduction of LECT2 expression was significantly correlated with the early recurrent and poor prognosis of the patient (P ¼ 0.024). To validate the ability of LECT2 to repress the growth of HCC, an adenoviral vector encoding the secreted human LECT2 (AdLECT2) was introduced into the human HCC cell lines Hep3B and PLC/PRF/5, which do not express endogenous LECT2. Over-expression of LECT2 resulted in the significant inhibition of in vitro migration and invasion of the AdLECT2-transfected HCC cells. Additionally, over-expression of AdLECT2 in subcutaneous Hep3B tumor xenografts in athymic nude mice resulted in significant inhibition of tumor growth (Po0.05). In summary, our data not only demonstrated that LECT2 is a candidate prognostic marker of human HCC, but also that therapeutic strategies targeting LECT2 expression is a promising therapy for human HCC.

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Human adenovirus type 2 protein IIIa I. Purification and characterization

Cited by 37 publications

References 31 publications

Polymer-coated adenovirus permits efficient retargeting and evades neutralising antibodies

Polymer-coated adenovirus permits efficient retargeting and evades neutralising antibodies

RICK Activates a NF-κB-dependent Anti-human Cytomegalovirus Response

The tumor suppressor function of LECT2 in human hepatocellular carcinoma makes it a potential therapeutic target

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