Human Adipose Tissue Cryopreservation: Impact of Different Calf Serum Concentrations on Tissue Viability

Gu, Luosha; Yin, Sun; Wang, Pu; Liu, Linbo; Gu, Jinsong

doi:10.1089/bio.2020.0038

Cited by 2 publications

(4 citation statements)

References 39 publications

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“…Abrupt cooling of such samples to ultra-low temperatures can lead to cell death and, as a result, to grafts having low viability after thawing [ 83 ]. Therefore, prior to freezing, the tissue is usually first cooled at low positive temperatures (4 °C) and only subsequently at negative temperatures (−20 °C) [ 110 , 111 ].…”

Section: Cryopreservation Of Tissue Specimen Of Msc Sourcesmentioning

confidence: 99%

“…It should be taken into account that their deferred studies in vivo may show completely different results [ 112 ]. Gu L. et al [ 110 ] used various concentrations of fetal calf serum (15% and 30%) in combination with 7.5% DMSO for adipose tissue cryopreservation. The beneficial effect on the viability of the thawed tissue of a large amount of serum (30%) in the cryoprotective mixture was demonstrated [ 110 ].…”

Section: Cryopreservation Of Tissue Specimen Of Msc Sourcesmentioning

confidence: 99%

“…As with the cryopreservation of cell suspensions, adipose tissue cryopreservation is most frequently conducted in line with the principle of slow freezing and fast thawing. The thawing protocol usually involves the water bath thawing of samples at 37 °C and removal of the cryoprotector by washing with a buffer solution, followed by stem cell isolation [ 10 , 73 , 107 , 110 , 111 , 118 , 119 ].…”

Section: Cryopreservation Of Tissue Specimen Of Msc Sourcesmentioning

confidence: 99%

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Cryostorage of Mesenchymal Stem Cells and Biomedical Cell-Based Products

Linkova

Rubtsova

Egorikhina

2022

Cells

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Mesenchymal stem cells (MSCs) manifest vast opportunities for clinical use due both to their ability for self-renewal and for effecting paracrine therapeutic benefits. At the same time, difficulties with non-recurrent generation of large numbers of cells due to the necessity for long-term MSC expansion ex vivo, or the requirement for repeated sampling of biological material from a patient significantly limits the current use of MSCs in clinical practice. One solution to these problems entails the creation of a biobank using cell cryopreservation technology. This review is aimed at analyzing and classifying literature data related to the development of protocols for the cryopreservation of various types of MSCs and tissue-engineered structures. The materials in the review show that the existing techniques and protocols for MSC cryopreservation are very diverse, which significantly complicates standardization of the entire process. Here, the selection of cryoprotectors and of cryoprotective media shows the greatest variability. Currently, it is the cryopreservation of cell suspensions that has been studied most extensively, whereas there are very few studies in the literature on the freezing of intact tissues or of tissue-engineered structures. However, even now it is possible to develop general recommendations to optimize the cryopreservation process, making it less traumatic for cells.

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Section: Cryopreservation Of Tissue Specimen Of Msc Sourcesmentioning

confidence: 99%

Section: Cryopreservation Of Tissue Specimen Of Msc Sourcesmentioning

confidence: 99%

Section: Cryopreservation Of Tissue Specimen Of Msc Sourcesmentioning

confidence: 99%

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Cryostorage of Mesenchymal Stem Cells and Biomedical Cell-Based Products

Linkova

Rubtsova

Egorikhina

2022

Cells

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“…Currently, there is no standardized cryoprotectant for preserving and storing human adipose tissue over the long term. Conventional cryoprotectants often contain dimethyl sulfoxide (DMSO), which is toxic to some extent, and the process of DMSO removal is intricate 14,15 . Therefore, it is needed to explore safe and non-toxic cryoprotectants with clear compositions.…”

mentioning

confidence: 99%

A Novel Clinical-Grade Cryopreservation Solution for Adipose Tissue Based on Metformin

Deng,

Liu,

Jian

et al. 2024

Preprint

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Background Autologous fat grafting often needs multiple sessions due to low volume retention. Young adipose tissue demonstrates a more pronounced therapeutic effect; thus, the cryopreservation of adipose tissue of young origin is particularly crucial. This study investigated the protective effect of a new cryopreservation solution combining trehalose, glycerol, and metformin on adipose tissue. Methods This study initially examined the effect of various concentrations of metformin (0, 1, 2, 4, and 8 mM) on oxidative damage in adipose tissue to identify the optimal concentration. Subsequently, 1.5 mL of fresh human adipose tissue was subjected to freezing using trehalose + glycerol (TG group), trehalose + glycerol + metformin (TGM group), and the common cryoprotectant dimethyl sulfoxide (DMSO) + fetal bovine serum (FBS) (DF group). Samples were cryopreserved in liquid nitrogen for 2 weeks. After thawing, 1 mL of adipose tissue from each group was transplanted subcutaneously into the backs of nude mice. The cryoprotective effects on adipose tissue viability were evaluated during transplantation one month after transplantation. Results The 2 mM concentration of metformin exhibited the lowest reactive oxygen species (ROS) level (29.20 ± 1.73) compared to other concentrations (P < 0.05). Cell proliferation and migration assays also supported the superior performance of the 2 mM concentration. Apoptotic analyses of SVF cells also showed the lowest levels in the 2 mM group. Compared to other cryopreservation groups, the adipose tissue in the TGM group closely resembled fresh adipose tissue in terms of gross structure and histological characteristics, with the lowest apoptosis rate of SVF cells. In vivo analysis revealed the highest tissue retention rate in the TGM group, with histological examination indicating robust structural integrity. Conclusion The TGM cryopreservation solution, containing metformin, greatly preserves adipose tissue, reduces apoptosis, and improves tissue retention rates. This solution was non-toxic and safe, making it well-suited for tissue cryopreservation in clinical settings.

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Human Adipose Tissue Cryopreservation: Impact of Different Calf Serum Concentrations on Tissue Viability

Cited by 2 publications

References 39 publications

Cryostorage of Mesenchymal Stem Cells and Biomedical Cell-Based Products

Cryostorage of Mesenchymal Stem Cells and Biomedical Cell-Based Products

A Novel Clinical-Grade Cryopreservation Solution for Adipose Tissue Based on Metformin

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