Human adipose tissue stromal vascular fraction cells differentiate depending on distinct types of media

Balwierz, Adriana; Czech, Urszula; Polus, Anna; Filipkowski, Robert K.; Mioduszewska, Barbara; Prószyński, Tomasz J.; Kołodziejczyk, Piotr; Skrzeczyńska‐Moncznik, Joanna; Dudek, Wojciech; Kaczmarek, Leszek; Kulig, J.; Pryjma, J; Dembińska-Kieć, A.

doi:10.1111/j.1365-2184.2008.00531.x

Cited by 27 publications

(31 citation statements)

References 43 publications

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“…These features permit a variety of stem cells and differentiated progeny, which respond to an assortment of signals that define the status of the skin and surrounding tissues. [22][23][24][25][26][27][28][29][30] Among these populations, mesenchymal stem cells have been shown to react to epithelial damage and assist in repair through a variety of mechanisms. The stromal vascular fraction, which harbors an assortment of mesenchymal stem cells/adipose-derived stem cells and progenitors, can be easily isolated, sustained in culture, and directed toward endothelial and angiogenic differentiation.…”

Section: Co-seeded Stromal Vascular Fraction To Lgr6 + Epithelial Stementioning

confidence: 99%

Transplantation of an LGR6+ Epithelial Stem Cell–Enriched Scaffold for Repair of Full-Thickness Soft-Tissue Defects

Lough¹,

Wetter

Madsen

et al. 2016

Plastic and Reconstructive Surgery

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Section: Co-seeded Stromal Vascular Fraction To Lgr6 + Epithelial Stementioning

confidence: 99%

Transplantation of an LGR6+ Epithelial Stem Cell–Enriched Scaffold for Repair of Full-Thickness Soft-Tissue Defects

Lough¹,

Wetter

Madsen

et al. 2016

Plastic and Reconstructive Surgery

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“…New adipocytes develop from the recruitment and differentiation of preadipocytes into fully differentiated adipocytes. The preadipocytes are precursors of fat cells and reside in vascular stroma of adipose tissues [109].…”

Section: Role Of Il-18 In Hiv-associated Lipodystro-phic Syndrome (Hals)mentioning

confidence: 99%

Potential Role of IL-18 in the Immunopathogenesis of AIDS, HIVAssociated Lipodystrophy and Related Clinical Conditions

Iannello¹,

Samarani²,

Débbeche³

et al. 2010

CHR

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IL-18 is a pleiotropic and multifunctional proinflammatory cytokine that is often produced in response to a viral infection. The biological activities of the cytokine are tightly controlled by its natural antagonist, IL-18 binding protein (IL-18BP), as well as by activation of caspase-1, which cleaves the precursor form of IL-18 into its biologically mature form. The cytokine plays an important role in both innate and adaptive antiviral immune responses. Depending upon the context, it can promote TH1, TH2 and TH17 responses. Increased serum concentrations of IL-18 and concomitantly decreased concentrations of its natural antagonist have been described in HIV-infected persons as compared to HIV-seronegative healthy subjects. We discuss in this review article how increased biological activities of IL-18 contribute towards immunopathogenesis of AIDS, HIV-associated lipodystrophy syndrome and related metabolic disturbances. While the advent of potent anti-HIV drugs has significantly enhanced life span of HIV-infected patients, it has also increased the number of these patients suffering from metabolic disorders. The cytokine may prove to be a useful target for therapeutic intervention in these patients.

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“…16,17 The ASCs isolated from homogenized adipose tissue can proliferate, and differentiate into adipocytes. [15][16][17][31][32][33][34] Because the process converted aspirated fat that was relatively poor in ASCs into ASCs-rich fat, the transplanted fat had improved viability and maintained a consistent volume. Although Yoshimura et al demonstrated the potential clinical use of their method for soft tissue augmentation in breast augmentation and facial lipoatrophy, they did not report a laboratory method for verification the adipogenesis of SVF.…”

Section: Introductionmentioning

confidence: 97%

Engineering Adipose Tissue from Uncultured Human Adipose Stromal Vascular Fraction on Collagen Matrix and Gelatin Sponge Scaffolds

Lin

Huang

Lin

et al. 2011

Tissue Engineering Part A

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Adipogenic potential was evaluated in uncultured stromal vascular fraction (SVF) loaded onto porous 3D collagen matrix and gelatin sponge scaffolds with predefined shapes. The SVF was isolated from 16 freshly lipectomized fat. Mean cell number was 6.0±4.68×10(7) cells/mL, and mean cell viability was 72%. Flow cytometric analysis revealed adipose-derived stromal cells (CD31(-), CD34(-/+), CD45(-), CD90(+), CD105(-), CD146(-), and CD166(+)) in the SVF. Three hours after harvest of fat, 200 μL of isolated SVF was loaded onto an experimental scaffold (4 mg in weight) and cultured in Dulbecco's modified Eagle's medium. Examination of the construct under an inverted light microscope and a scanning electron microscope demonstrated adequate seeding and active proliferation of the SVF cells on pore surfaces. Cells grew to varying sizes in clusters or in strands. On day 28, histologic study of the constructs by H&E staining revealed viable adipocytes in the microstructure. Positive Oil-Red O stain confirmed lipid accumulation in mature adipocytes. The presence of human adipocytes was further assessed by the presence of genomic DNA detected by GAPDH gene and by the mRNA expression of peroxisome proliferator activated receptor-γ, and lipoprotein lipase. The results demonstrated that new adipose tissue can be regenerated by seeding freshly isolated, uncultured SVF on 3D porous collagen matrix and gelatin sponge scaffolds.

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Human adipose tissue stromal vascular fraction cells differentiate depending on distinct types of media

Abstract: Here, we show that differentiation of SVF cells to endothelial cells or adipocyte-like cells depended on the medium used. Our work provides a clear model for analysing the differentiation capacity of SVF cells.

Cited by 27 publications

References 43 publications

Transplantation of an LGR6+ Epithelial Stem Cell–Enriched Scaffold for Repair of Full-Thickness Soft-Tissue Defects

Transplantation of an LGR6+ Epithelial Stem Cell–Enriched Scaffold for Repair of Full-Thickness Soft-Tissue Defects

Potential Role of IL-18 in the Immunopathogenesis of AIDS, HIVAssociated Lipodystrophy and Related Clinical Conditions

Engineering Adipose Tissue from Uncultured Human Adipose Stromal Vascular Fraction on Collagen Matrix and Gelatin Sponge Scaffolds

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