Human amniotic fluid: a source of stem cells for possible therapeutic use

Dziadosz, Margaret; Basch, Ross S.; Young, Bruce K.

doi:10.1016/j.ajog.2015.12.061

Cited by 43 publications

(30 citation statements)

References 56 publications

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“…Stem cells are typically characterized by the presence of surface markers associated with self‐renewal without differentiation. Flow cytometry is a fundamental technique in the identification of stem cells through detection of these markers . The general strategy when identifying animal MSCs in vitro includes the detection of molecular markers proposed by ISCT together with markers commonly used to describe MSCs in other species.…”

Section: Discussionmentioning

confidence: 99%

“…Flow cytometry is a fundamental technique in the identification of stem cells through detection of these markers. 83 The general strategy when identifying animal MSCs in vitro includes the detection of molecular markers proposed by ISCT 21 together with markers commonly used to describe MSCs in other species. Here, the majority of rAF-MSCs expressed CD29 and CD44 surface markers, whereas a negative expression of CD105 and CD166 was observed.…”

Section: Discussionmentioning

confidence: 99%

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Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Kováč

Vašíček

Kulíková

et al. 2017

Biotechnology Progress

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Over the years, there has been much confusion in defining molecular markers of mesenchymal stem cells (MSCs) for other than human species due to a lack of species-specific antibodies. Therefore, the aim of our study was to define rabbit amniotic fluid-derived mesenchymal stem cells (rAF-MSCs) and to reflect upon the current identification of AF-MSCs by providing a summary of detected surface markers in different species. The expression of rAF-MSC surface markers was analyzed at the protein and mRNA level. Flow cytometry analyses showed that rAF-MSCs were positive for CD29 and CD44, low positive for CD90, but negative for CD73, CD105, and CD166. Interestingly, RT-PCR (reverse transcription-polymerase chain reaction) exposed a discprepancy between transcribed mRNA and protein expression, as the cells expressed mRNA of all MSC markers: CD29, CD44, CD73, CD90, CD105, and CD166. Our results also confirmed the mesenchymal nature of isolated cells by morphology, ultrastructure, and intracellular marker expression profile. In addition, the expression of few pluripotency markers was also detected. We also found that passaging did not affect apoptosis and viability. Similarly, changes in karyotype were not observed during passaging. In conclusion, the provided characteristics may be used as a comprehensive set of criteria to define and characterize rAF-MSCs, required for the identification of these cells in preclinical investigations. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1601-1613, 2017.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Kováč

Vašíček

Kulíková

et al. 2017

Biotechnology Progress

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“…The human amniotic fluid contains self-renewing multipotent amniotic MSCs (AFSCs)6, which are characterized by their spindle-shape fibroblastic morphology, plastic adherence, expression of the cell surface markers CD105, CD73, CD90, CD19, and absence of expression of CD34, CD45, and CD2978. AFSCs are attractive candidates for cell therapy because they are easily accessible during pregnancy from the surplus of amniocentesis samples and can be used without ethical restriction9101112. They also have a high expansion potential, are non-tumorigenic, tolerogenic, anti-inflammatory and are small enough to pass through capillary beds to reach distant sites of action13141516.…”

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confidence: 99%

Counteracting bone fragility with human amniotic mesenchymal stem cells

Ranzoni

Corcelli

Hau

et al. 2016

Sci Rep

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The impaired maturation of bone-forming osteoblasts results in reduced bone formation and subsequent bone weakening, which leads to a number of conditions such as osteogenesis imperfecta (OI). Transplantation of human fetal mesenchymal stem cells has been proposed as skeletal anabolic therapy to enhance bone formation, but the mechanisms underlying the contribution of the donor cells to bone health are poorly understood and require further elucidation. Here, we show that intraperitoneal injection of human amniotic mesenchymal stem cells (AFSCs) into a mouse model of OI (oim mice) reduced fracture susceptibility, increased bone strength, improved bone quality and micro-architecture, normalised bone remodelling and reduced TNFα and TGFβ sigalling. Donor cells engrafted into bones and differentiated into osteoblasts but importantly, also promoted endogenous osteogenesis and the maturation of resident osteoblasts. Together, these findings identify AFSC transplantation as a countermeasure to bone fragility. These data have wider implications for bone health and fracture reduction.

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“…Stem cells are typically characterized by the presence of surface markers associated with self-renewal without differentiation. Flow cytometry is a fundamental technique in the identification of stem cells through detection of these markers (Dziadosz et al, 2016). In this preliminary study, rAF-MSCs highly expressed CD29 and CD44.…”

Section: Resultsmentioning

confidence: 99%

Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit

Kováč¹,

Vašíček²,

Kulíková³

et al. 2017

JCEA

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The isolation of amniotic fluid-derived mesenchymal stem cells (AF-MSCs) has been already shown in human and several other species including rabbit. However, prior to the preclinical research on various animal models it is desirable to define AF-MSCs by a panel of surface protein markers. Therefore, the aim of this preliminary study was to detect the expression of several protein markers on the surface of AF-MSCs isolated from local breed of Nitra rabbit. Amniotic fluid was collected from humanely sacrificed rabbits (n = 3) and AF-MSCs were cultured to a third passage. Flow cytometry was used to detect surface protein marker expression and for viability testing. Rabbit AF-MSCs (rAF-MSCs) were also analyzed by transmission electron microscopy to define the ultrastructure. rAF-MSCs showed both sufficient viability (more than 80%) and low apoptosis rates at third passage and highly expressed CD29 (88.17 ± 7.17%) and CD44 (80.00 ± 2.28%). However, a dim expression of CD90 (17.24 ± 1.31%) and negative expression of CD73 (1.21 ± 0.56% and 4.41 ± 1.46%), CD105 (1.67 ± 0.37%) and CD166 (0.96 ± 2.26%) was observed. Additionally, ultrastructure analysis revealed eccentrically located nucleoli, an abundance of thin pseudopodia on cells' surfaces and proved the presence of typical mesenchymal stem cell features. In conclusion, this set of data contributes to more detailed information on rAF-MSCs, which were previously proposed feasible for preclinical stem cell research and as a suitable source for the cryopreservation of animal genetic resources in gene bank.

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Human amniotic fluid: a source of stem cells for possible therapeutic use

Cited by 43 publications

References 56 publications

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Counteracting bone fragility with human amniotic mesenchymal stem cells

Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit

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