Human amoebiasis in Multan, Punjab, Pakistan

Tasawar, Zahida; Lashari, Mushtaq Hussain; Anjum, Asma; Aziz, Fariha

doi:10.5897/jcab2013.0377

Cited by 4 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Over all prevalence of intestinal parasites seen in present study was higher than those reported in Pakistan [6,7,8,9,19,11,12,13,20,21]. This is due to the fact that these studies dealt with targeted groups i.e.…”

Section: Discussioncontrasting

confidence: 54%

Prevalence and Risk Factors Associated with Intestinal Parasitic Infections among Food Handlers of Swat, Khyber Pakhtunkhwa, Pakistan

Khan¹,

Noor-Un-Nisa²,

Khan³

2017

JFNR

View full text Add to dashboard Cite

Intestinal parasitic infection is one of the public health problems in developing countries including Pakistan. Food handlers being major source, therefore effective prevention and control require the identification of local risk factors. Presently occupation based cross sectional study was conducted to assess the prevalence of intestinal parasitic infections and associated risk factors among food handlers of district Swat, Khyber Pakhtunkhwa, Pakistan from January 2011 to December 2013. Study subjects were selected based on random sampling methods. A total of 267 food handlers were enrolled, socio-demographic data and possible factors for the occurrence of intestinal parasitic infection were collected using a pre-tested structured questionnaire. Approximately 10 grams of stool specimens were examined using wet mount and formal ether concentration technique. The overall prevalence of intestinal parasite was 83.1% (n=222/267). Multiple infections were identified in 28.4% (n=76/267) individuals. These infections were given in order of their frequency and percentage as: Ascaris lumbricoides 55.8% (n=149/267) Trichuris trichuira 14.9% (n=40/267), Entamoeba histolytica /dispar 14.2% (38/267), Enterobius vermicularis 9.73% (n=26/267), Hymenolepis nana 9.36% (n=25/267), Taenia saginata 8.98% (n=24/267), Hookworm 5.99% (16/267) and Giardia lamblia 5.61% (n=15/267). Hand washing before food handling, preparing food during suffering from infectious diseases and using common knife for cutting the flesh or vegetable were the most significantly associated factors for the occurrence of intestinal parasite infection. The results highlighted that food handlers with different pathogenic organisms may influence consumers to significant health risks. Routine screening and treatment of food handlers is a proper tool in preventing food-borne parasitic diseases.

show abstract

Section: Discussioncontrasting

confidence: 54%

Prevalence and Risk Factors Associated with Intestinal Parasitic Infections among Food Handlers of Swat, Khyber Pakhtunkhwa, Pakistan

Khan¹,

Noor-Un-Nisa²,

Khan³

2017

JFNR

View full text Add to dashboard Cite

show abstract

“…The overall prevalence of Entamoeba was recorded 27.75% (111/400). According to a previous study conducted in Multan (Punjab) Pakistan the prevalence of Entamoeba was recorded as 7.09% in age group 1-16 years [26]. A study from District Swat Pakistan, reported that 70 patients out 100 were infected with E. histolytica [27].…”

Section: Discussionmentioning

confidence: 99%

Differential detection of Entamoeba species in stool samples collected from children in District Swat, Khyber Pakhtunkhwa Pakistan

Khan

Shams

Khan

et al. 2019

Preprint

View full text Add to dashboard Cite

29Background: Amoebiasis is an intestinal disease caused by enteric protozoan called 30 Entamoeba histolytica belongs to the Genus Entamoeba. The main reason of infection is the 31 contamination of food and water due to the poor sanitation. Among Entamoeba species, 32 Entamoeba histolytica is highly pathogenic while the other species are non-pathogenic and 33 needs no medical treatment. 34Methodology: A total of 400 stool samples were collected from different areas of 35 district Swat and were processed for screening of amoebic cells. Microscopically identified 36 samples containing amoebic cells were stored at -20 o C till DNA extraction. Extracted DNA 37 was used in a PCR reaction with specific reference primers to amplify the target DNA. 38 Results: Out of all 400 stool samples 111 (27.7%) were found positive through 39 microscopy while PCR reaction confirmed 80 out of microscope positive samples. Among 80 40 PCR positive samples, the infection with Entamoeba dispar was most common (57.5%) 41followed by E. histolytica (47.5%) and Entamoeba moshkovskii (20%). The positive cases for 42 mono-infection of E. dispar were 33 (41.25%), followed by E.histolytica 25 (31.25%) and E. 43 moshkovskii 7 (8.75%). The co-infection of E. histolytica with E. dispar and E. moshkovskii 44 was 6 (7.5%) and 2 (2.5%), respectively. Similarly the co-infection of Entamoeba dispar with 45 Entamoeba moshkovskii was also 2 (2.5%) while 5 (6.25%) samples were observed with 46 mixed infection of E. histolytica, E. dispar and E. moshkovskii. 47 Significance of the study: The aim of the study was to detect and differentiate the 48 E. histolytica, Entamoeba dispar and Entamoeba moshkovskii using conventional microscopy 49 and polymerase chain reaction. The results suggested that the use of PCR is necessary to 50 differentiate E. histolytica from E. dispar and E. moshkovskii and therefore, to avoid 51 unnecessary treatment the present study recommend the use of PCR for the routine diagnosis 52 of amoebiasis in the study area. It is also suggested that further studies from this area may 53 also facilitate the understanding of genetic diversity of these pathogens. 54 55 56 57 58 59 60 61 62 63 65Entamoeba histolytica is an enteric protozoan belongs to genus Entamoeba which is 66 the causative agent of amoebiasis [1]. The Genus Entamoeba comprises of several species 67 among them six reside in human intestinal tract including E. histolytica, E. dispar, E. 68 moshkovskii, E. coli, E. poleki and E. hartmanni [2]. There are two forms Entamoeba, the 69 trophozoite which has short life span and are mobile that can invade the different organ 70 systems, while the cysts are long surviving form of Entamoeba that colonize the patient. The 71 cyst consists of resistant walls which protect these species from desiccation in external 72 environment [3]. In external environment the cysts can survive for several days, weeks or 73 month mostly in damp conditions and are responsible for transmission of infection [4].74 The transmission of ...

show abstract

Seasonal survey, risk factor's analysis and genotyping of Theileria annulata infecting cattle in Punjab province, Pakistan

et al. 2022

View full text Add to dashboard Cite

Human amoebiasis in Multan, Punjab, Pakistan

Cited by 4 publications

References 30 publications

Prevalence and Risk Factors Associated with Intestinal Parasitic Infections among Food Handlers of Swat, Khyber Pakhtunkhwa, Pakistan

Prevalence and Risk Factors Associated with Intestinal Parasitic Infections among Food Handlers of Swat, Khyber Pakhtunkhwa, Pakistan

Differential detection of Entamoeba species in stool samples collected from children in District Swat, Khyber Pakhtunkhwa Pakistan

Seasonal survey, risk factor's analysis and genotyping of Theileria annulata infecting cattle in Punjab province, Pakistan

Contact Info

Product

Resources

About