Human and Bovine Dentin Composition and Its Hybridization Mechanism Assessed by FT-Raman Spectroscopy

Abstract

FT-Raman spectroscopy was used to study the human and bovine dentin and their interactions with adhesive systems. Ten human (H) molars and ten bovine (B) teeth were prepared exposing the dentin and then each specimen was divided into two parts. The resulted forty dentin segments were treated either with the total-etch one bottle adhesive (Prime & Bond 2.1, PB) or with the single-step self-etching adhesive (Xeno III, X) and divided into four groups: HPB (control), HX, BPB, and BX. Each group was analyzed by FT-Raman spectroscopy before and after the adhesive treatment. Six regions of the Raman spectrum were analyzed and the integrated areas of organic and inorganic peaks were calculated. Bovine untreated specimens showed higher peak area ofPO4 3−ν2 content than in human specimens. Human untreated specimens had higher peak areas ofPO4 3−ν4andCO3 2−ν1 contents than in bovine specimens. The peak areas of amide III,CH2, and amide I contents were higher in human than in bovine specimens (before treatments). Treated dentin showed no significant statistical differences between the adhesives for both inorganic and organic contents considering the same substrate. However, the differences found between human and bovine specimens after adhesives application show a reduced accuracy of these substrates as a substitute to the human specimens.

Resistance to fracture of intraradicular posts made of biological materials

“…It has been found that human teeth are morphologically and histologically similar to teeth from other mammals [ 11 ]. The composition of human enamel and dentin is similar to the composition of bovine dental structures [ 12 ].…”

“…The composition of human enamel and dentin is similar to the composition of bovine dental structures [ 12 ]. It has been reported that the adhesion to the superficial layer of dentin showed no significant difference between human and bovine dentin [ 11 ]. The morphology of coronal dentin and enamel is similar when comparing bovine and human teeth.…”

“…The morphology of coronal dentin and enamel is similar when comparing bovine and human teeth. Moreover, bovine teeth provide other advantages, such as greater availability, larger dimensions, and radiodensity similar to human dentin [ 11 – 13 ]. In addition, ethics committees around the world have stimulated the replacement of human teeth with animal ones for researches purposes [ 11 ].…”

Effect of Lesion Baseline Severity and Mineral Distribution on Remineralization and Progression of Human and Bovine Dentin Caries Lesions

“…Higher radiodensity was reported for bovine than for human dentin [Fonseca et al, 2008], although a similar study [Tanaka et al, 2008] came to the opposite conclusion. Investigations into the tissues' mineral content could not be retrieved, although a recent study [Soares et al, 2013] hinted at some differences in organic and inorganic content between dentin substrates. Furthermore, only very few comparative studies on human and bovine root caries lesion pro-and regression have been reported.…”

Influência de adesivos autocondicionantes na resistência adesiva de cimentos de ionômero de vidro resinomodificados à dentina

“…Mesmo assim, ainda não há um consenso na literatura quanto à utilização dos dentes bovinos como substituto dos dentes humanos nos testes de adesão. Apesar desses substratos apresentam características morfológicas e histológicas semelhantes (Saunders, 1988;Tagami et al, 1989), estudos recentes têm demonstrado que existem diferenças microestruturais entre o substrato humano e bovino (Bonfim, 2000;Soares et al, 2013), por isso variáveis como a região do dente em que os testes são realizados, morfologia, composição e diferenças nas propriedades físicas dos substratos bovino e humano devem ser levadas em consideração para a correta interpretação dos dados obtidos a partir de estudos empregando dentes bovinos (Yassen et al, 2011;Santo, 2015). Matos et al (2008) relatam que apenas as regiões centrais da dentina bovina apresentam os túbulos dentinários dispostos regularmente.…”