Human and rodent humoral immune responses to Andes virus structural proteins

Tischler, Nicole D.; Galeno, H.; Rosemblatt, Mario; Valenzuela, Pablo

doi:10.1016/j.virol.2005.01.031

Cited by 33 publications

(32 citation statements)

References 37 publications

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“…After onset of the acute phase IgM as well as IgG antibodies can be detected that react with hantaviral N protein, which represents the major target antigen. [28][29][30][31][32][33][34][35] Antibodies against Gc and Gn appear later during the progress of disease. 36 It remains still enigmatic why hantaviral N protein is the dominant antigen.…”

Section: Human Adaptive Immune Responses To Hantavirusesmentioning

confidence: 99%

Human pathogenic hantaviruses and prevention of infection

2011

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Section: Human Adaptive Immune Responses To Hantavirusesmentioning

confidence: 99%

Human pathogenic hantaviruses and prevention of infection

2011

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“…Altogether, these features account for the main reason of hantavirus N protein detection for endpoint ELISA or immunofluorescence microscopy assays to assess the amount of viral particles and the progression of viral infection (Alexeyev et al, 1996). The detection of N is also used to quantify the amount of infectious particles in focus assays (Bharadwaj et al, 2000;McCormick et al, 1982;Niklasson et al, 1991;Tanishita et al, 1984;Tischler et al, 2005). This last assay employs a semisolid overlay that is applied after virus adsorption, in order to limit virus replication and spread to the initial sites of infection, forcing the virus growth in foci.…”

Section: Short Communicationmentioning

confidence: 99%

“…To test if the developed flow cytometry assay allowed reliable titration of virus stocks, different batches and dilutions thereof were analyzed and compared with results obtained by traditional focus assay (Tischler et al, 2005). Virus titers obtained from flow cytometry titration assays were calculated within the linear range spanning values between 1 and 40 % of infected cells, similar to the range established for other viruses (Drayman and Oppenheim, 2011).…”

Section: Short Communicationmentioning

confidence: 99%

“…For this purpose, ANDV was pre-incubated with different sera derived from ANDV infected patients. These sera were characterized previously concerning their activity to neutralize ANDV and simian immunodeficiency virus particles pseudotyped with ANDV glycoproteins (Tischler et al, 2005;Cifuentes-Muñoz et al, 2010). Each of the tested patient serum diminished virus infection over 75 % while viral infectivity was not reduced by the negative control serum (Fig.…”

Section: Short Communicationmentioning

confidence: 99%

“…and focus assay using ANDV N for immunodetection. The focus assay was performed as previously described (Tischler et al, 2005). B) Neutralization of ANDV infectivity by sera of ANDV infected patients.…”

Section: Short Communicationmentioning

confidence: 99%

See 2 more Smart Citations

A rapid method for infectivity titration of Andes hantavirus using flow cytometry

Barriga¹,

Martínez-Valdebenito²,

Galeno³

et al. 2013

Journal of Virological Methods

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Prediction of B Cell Epitopes Among Hantavirus Strains Causing Hemorragic Fever With Renal Syndrome

Sagadevan

Sankar

Ramamurthy

et al. 2016

J of Cellular Biochemistry

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Hantavirus infections are now recognized to be a global problem. The hantaviruses include several genotypic variants of the virus with different distributions in varying geographical regions. The virus genotypes seem to segregate in association with certain manifestations specific for each syndrome. They primarily include HFRS, HCPS, febrile illness with or without mild involvement of renal diseases. In the course of our study on hantavirus etiology of febrile illnesses, we recovered a hantavirus strain identified by nPCR. This has been sequenced to be Hantaan-like virus (partial S segment). The current manuscript is focused on understanding the N protein coded by S segment in terms of variation of amino acid sequences of the virus genotypes associated with HFRS. The diagnosis of this infection is achieved by PCR testing of serum/plasma or demonstration of IgM/IgG in serum. The limitations of PCR are temporal often not positive after 7 days of onset of infection. IgM detection is possible around this period and up to 21 days. IgG detection is less definitive in acute infections. Here, we report characterization of the sequence diversity of HFRS strains, 3D structure of Hantaan N protein, and B-cell epitopes on this molecule. We predicted a 20 amino acid sequence length peptide by using BepiPred online server in IEDB analysis resource program. We suggest this peptide may be used for development of geographic region-specific immunoassays like EIAs for antibody detection, monoclonal antibody development, and immunoblots (line immunoassay). J. Cell. Biochem. 118: 1182-1188, 2017. © 2016 Wiley Periodicals, Inc.

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Human and rodent humoral immune responses to Andes virus structural proteins

Cited by 33 publications

References 37 publications

Human pathogenic hantaviruses and prevention of infection

Human pathogenic hantaviruses and prevention of infection

A rapid method for infectivity titration of Andes hantavirus using flow cytometry

Prediction of B Cell Epitopes Among Hantavirus Strains Causing Hemorragic Fever With Renal Syndrome

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