Human anti-HLA monoclonal antibodies: Production, characterization, and application

Pistillo, Maria Pia; Mazzoleni, Osvaldo; Tanigaki, Nobuyuki; Hämmerling, Ulrich; Longo, Anna; Frumento, Guido; Ferrara, G. B.

doi:10.1016/0198-8859(88)90035-3

Cited by 20 publications

(8 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Multiparous female animals often develop alloreactive antibody responses to non-self-epitopes that distinguish individual MHC allomorphs rather than framework epitopes present on all MHC molecules [10]. To stimulate production of Mamu-A1*001-binding antibodies, we alloimmunized a Mamu-A1*001-negative macaque mother of multiple Mamu-A1*001-positive offspring with a lymphocyte immunotherapy regimen previously employed to treat humans for recurrent spontaneous abortions (Fig 1) [31].…”

Section: Resultsmentioning

confidence: 99%

“…However, this approach is difficult when isolating monoclonal antibodies specific for variants of primate polymorphic proteins since mice preferentially target xenogeneic framework determinants [10]. We overcame this hurdle by alloimmunizing a rhesus macaque with PBMC from an MHC disparate macaque to induce MHC allomorph-specific antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…Advances in immortalizing human B cells and the development of phage-display technologies allowed for the isolation of anti-HLA monocolonal antibodies from allosensitized individuals [10, 11]. The anti-HLA antibodies have proven to be valuable reagents for investigating MHC molecules themselves and their role in disease processes.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation of a monoclonal antibody from a phage display library binding the rhesus macaque MHC class I allomorph Mamu-A1*001

et al. 2017

View full text Add to dashboard Cite

Monoclonal antibodies that bind to human leukocyte antigen (HLA) are useful tools for HLA-typing, tracking donor-recipient chimerisms after bone marrow transplants, and characterizing specific major histocompatibility complexes (MHC) on cell surfaces. Unfortunately, equivalent reagents are not available for rhesus macaques, which are commonly used animal as models in organ transplant and infectious disease research. To address this deficiency, we isolated an antibody that recognizes the common Indian rhesus macaque MHC class I molecule, Mamu-A1*001. We induced Mamu-A1*001-binding antibodies by alloimmunizing a female Mamu-A1*001-negative rhesus macaque with peripheral blood mononuclear cells (PBMC) from a male Mamu-A1*001-positive donor. A Fab phage display library was constructed with PBMC from the alloimmunized macaque and panned to isolate an antibody that binds to Mamu-A1*001 but not to other common rhesus macaque MHC class I molecules. The isolated antibody distinguishes PBMC from Mamu-A1*001-positive and -negative macaques. Additionally, the Mamu-A1*001-specific antibody binds the cynomolgus macaque MHC class I ortholog Mafa-A1*001:01 but not variants Mafa-A1*001:02/03, indicating a high degree of binding specificity. The Mamu-A1*001-specific antibody will be useful for identifying Mamu-A1*001-positive rhesus macaques, for detecting Mamu-A1*001-positive cells in populations of Mamu-A1*001-negative cells, and for examining disease processes that alter expression of Mamu-A1*001 on cell surfaces. Moreover, the alloimmunization process we describe will be useful for isolating additional MHC allomorph-specific monoclonal antibodies or antibodies against other polymorphic host proteins which are difficult to isolate with traditional technologies.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of a monoclonal antibody from a phage display library binding the rhesus macaque MHC class I allomorph Mamu-A1*001

et al. 2017

View full text Add to dashboard Cite

show abstract

“…These include antibodies recognizing subpopulations of T cells [1], new major histocompatibility complex (MHC) antigens [17], and well defined MHC products such as HLA-B15 [26], HLA Dr W52 and W53 [33,36], and DQ Wl, W2 and W3 [25,32,46]. The production, characterization, application, and advantages of such antibodies have recently been reviewed [45].…”

Section: Human Monoclonals and Transplantationmentioning

confidence: 99%

Human Monoclonal Antibody Technology—Are its Achievements, Challenges, and Potential Appreciated?

James

1989

Scand J Immunol

View full text Add to dashboard Cite

“…several DR-and DQ-specific monoclonal antibodies (Pistillo et al 1986(Pistillo et al , 1987(Pistillo et al , 1988. A stable monoclonal cell line MP8 that secretes a supertypic DR-specific antibody was generated by EBV transformation, cluster picking, and cloning from peripheral B lymphocytes of healthy individual SG (DR5,w8,w52; DQw3; DPB4.1), who had been repeatedly immunized with peripheral blood lymphocytes from a single donor MG (DR3,w6;DQwl,w2;DPB4.2,DPBIO).…”

mentioning

confidence: 99%

Human monoclonal antibody MP8 detects a supertypic determinant encoded byDPB allelesDPB2.1, DPB3, DPB4.2, DPB8, DPB9, DPB10, andDPB14

et al. 1989

Self Cite

View full text Add to dashboard Cite

Human anti-HLA monoclonal antibodies: Production, characterization, and application

Cited by 20 publications

References 48 publications

Isolation of a monoclonal antibody from a phage display library binding the rhesus macaque MHC class I allomorph Mamu-A1*001

Isolation of a monoclonal antibody from a phage display library binding the rhesus macaque MHC class I allomorph Mamu-A1*001

Human Monoclonal Antibody Technology—Are its Achievements, Challenges, and Potential Appreciated?

Human monoclonal antibody MP8 detects a supertypic determinant encoded byDPB allelesDPB2.1, DPB3, DPB4.2, DPB8, DPB9, DPB10, andDPB14

Contact Info

Product

Resources

About