Human Anulus Fibrosis and Nucleus Pulposus Cells of the Intervertebral Disc

Kluba, Torsten; Niemeyer, Thomas; Gaissmaier, Christoph; Gründer, Tatiana

doi:10.1097/01.brs.0000192204.89160.6d

Cited by 122 publications

(120 citation statements)

References 22 publications

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“…It was previously shown by Kluba et al (2005) that collagen type II expression is reduced during monolayer culture, a finding that we were able to confirm. However, it is known that disc cell culture in appropriate 3D structures as the pellet system may at least partly revert to their original features (Gan et al, 2003).…”

Section: Discussionsupporting

confidence: 77%

“…While aggrecan expression remained unchanged in all culture systems, substantial changes were found for collagen expression. Collagen type II expression of biopsies was downregulated during monolayer expansion, data obtained being similar to that presented by Kluba et al (2005). However, we were able to demonstrate clearly that collagen type II expression in 3D cultures, PU-fibrin structure and pellet was upregulated compared to monolayer cultures.…”

Section: Discussionsupporting

confidence: 64%

“…Various materials have been studied in vitro, and cultured with animal or human cells obtained either from the AF or NP (Yang et al, 2005;Revell et al, 2007;Halloran et al, 2008;Kluba et al, 2005;Gruber et al, 2004). However, most of the materials tested so far fulfill only some of the criteria required but fail in others.…”

Section: Discussionmentioning

confidence: 99%

“…To overcome the disadvantages of a simple cellsolution injection, various studies have been performed using cells embedded in gel-like matrices (poly-L lactic acid, gelatin, collagen-hyaluronan, alginate, chitosan, fibrin) (Brown et al, 2005;Roughley et al, 2006;Halloran et al, 2008;Richardson et al, 2006;Kluba et al, 2005;Yang et al, 2008). Although these studies revealed that the correct matrix formation (including aggrecan, collagen type I and II) can be displayed in the matrix material, the hydrogels do not supply the required initial mechanical stability to the implanted structure.…”

Section: Discussionmentioning

confidence: 99%

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Cell-seeded polyurethane-fibrin structures – A possible system for intervertebral disc regeneration

Mauth

Bono²,

Haas³

et al. 2009

eCM

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Nowadays, intervertebral disc (IVD) degeneration is one of the principal causes of low back pain involving high expense within the health care system. The long-term goal is the development of a medical treatment modality focused on a more biological regeneration of the inner nucleus pulposus (NP). Hence, interest in the endoscopic implantation of an injectable material took center stage in the recent past. We report on the development of a novel polyurethane (PU) scaffold as a mechanically stable carrier system for the reimplantation of expanded autologous IVDderived cells (disc cells) to stimulate regenerative processes and restore the chondrocyte-like tissue within the NP. Primary human disc cells were seeded into newly developed PU spheroids which were subsequently encapsulated in fibrin hydrogel. The study aims to analyze adhesion properties, proliferation capacity and phenotypic characterization of these cells. Polymerase chain reaction was carried out to detect the expression of genes specifically expressed by native IVD cells. Biochemical analyses showed an increased DNA content, and a progressive enhancement of total collagen and glycosaminoglycans (GAG) was observed during cell culture. The results suggest the synthesis of an appropriate extracellular matrix as well as a stable mRNA expression of chondrogenic and/or NP specific markers. In conclusion, the data presented indicate an alternative medical approach to current treatment options of degenerated IVD tissue.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Cell-seeded polyurethane-fibrin structures – A possible system for intervertebral disc regeneration

Mauth

Bono²,

Haas³

et al. 2009

eCM

View full text Add to dashboard Cite

show abstract

“…However, it is unclear if chondrocyte-like NP cells change in the same way during expansion (Kluba et al, 2005). Preserving maximal expression of ECM proteins in NP cells during culture is important to maximise the effectiveness of autologous cell implantation therapy in the NP of degenerate IVDs.…”

Section: Continuous Expansion Culture Of Np Cellsmentioning

confidence: 99%

Comparative analysis in continuous expansion of bovine and human primary nucleus pulposus cells for tissue repair applications

Rosenzweig¹,

Gravel²,

Bisson³

et al. 2017

eCM

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Autologous NP cell implantation is a potential therapeutic avenue for intervertebral disc (IVD) degeneration. However, monolayer expansion of cells isolated from surgical samples may negatively impact matrix production by way of dedifferentiation. Previously, we have used a continuous expansion culture system to successfully preserve a chondrocyte phenotype. In this work, we hypothesised that continuous expansion culture could also preserve nucleus pulposus (NP) phenotype. We confirmed that serial passaging drove NP dedifferentiation by significantly decreasing collagen type II, aggrecan and chondroadherin (CHAD) gene expression, compared to freshly isolated cells. Proliferation, gene expression profile and matrix production in both culture conditions were compared using primary bovine NP cells. Both standard culture and continuous culture produced clinically relevant cell populations. However, continuous culture cells maintained significantly higher collagen type II, aggrecan and CHAD transcript expression levels. Also, continuous expansion cells generated greater amounts of proteoglycan, collagen type II and aggrecan protein deposition in pellet cultures. To our surprise, continuous expansion of human intervertebral disc cells -isolated from acute herniation tissue -produced less collagen type II, aggrecan and CHAD genes and proteins, compared to standard culture. Also, continuous culture of cells isolated from young non-degenerate tissue did not preserve gene and protein expression, compared to standard culture. These data indicated that primary bovine and human NP cells responded differently to continuous culture, where the positive effects observed for bovine cells did not translate to human cells. Therefore, caution must be exercised when choosing animal models and cell sources for pre-clinical studies.

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Enhancing annulus fibrosus tissue formation in porous silk scaffolds

Chang

Kim

Vunjak-Novakovic

et al. 2009

J Biomedical Materials Res

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There is presently no optimal treatment for patients with chronic back pain as a result of degenerative disc disease. Tissue engineering, an annulus fibrosus (AF) construct suitable to repair the damaged AF, is one novel approach to the treatment of this disease. We have previously demonstrated that porous silk scaffolds can support AF cell attachment and extracellular matrix accumulation; however, tissue infiltration and matrix accumulation was not optimal. The purpose of this study was to determine whether the dynamic culture of AF cells seeded into larger average pore size silk scaffolds would improve tissue formation. AF cells were isolated from bovine caudal discs and seeded into porous silk scaffolds and grown in either dynamic or static flow conditions. The cell-seeded scaffolds were grown for up to 4 weeks and evaluated for cell attachment, gene expression, histological appearance, and matrix accumulation. Dynamic culture improved AF tissue formation as the tissue was more cellular and contained significantly more matrix than that formed in static culture. Spatial distribution of tissue was comparable for static and dynamic culture. Varying scaffold pore sizes (200-, 600-, and 1000-microm pore size) demonstrated that an average pore size of 600 microm resulted in the most uniform tissue distribution with the greatest amount of type I collagen. Our study suggests that dynamic flow conditions and scaffold pore size can affect the formation of engineered AF tissue.

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Human Anulus Fibrosis and Nucleus Pulposus Cells of the Intervertebral Disc

Abstract: These results provide a better understanding of how the phenotype of human healthy and degenerative disc cells is influenced by in vitro expansion. This may be useful for future tissue engineering purposes.

Cited by 122 publications

References 22 publications

Cell-seeded polyurethane-fibrin structures – A possible system for intervertebral disc regeneration

Cell-seeded polyurethane-fibrin structures – A possible system for intervertebral disc regeneration

Comparative analysis in continuous expansion of bovine and human primary nucleus pulposus cells for tissue repair applications

Enhancing annulus fibrosus tissue formation in porous silk scaffolds

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