Human γδ T‐cell recognition of Yersinia enterocolitica

Abstract

SUMMARYWe have studied the human cd T-cell response to Yersinia enterocolitica, a facultative intracellular bacterium which causes gastroenteritis and, particularly in human leucocyte antigen (HLA)-B27+ individuals, reactive arthritis (ReA). A marked proliferation of that cytotoxic cd T cells is seen when Yersinia-infected lymphoblastoid cell lines or fixed intact Yersinia are added to cultures of mononuclear cells derived from the synovial fluid of ReA patients or from the peripheral blood of healthy donors. In contrast, heat-inactivated Yersinia fail to stimulate the cd T-cell response. The cd T-cell lines generated killed both autologous and allogeneic infected cell lines. Interestingly, a T-cell line generated from synovial fluid mononuclear cells (SFMC ) killed infected autologous cell lines and a cell line matched for HLA-B27 less well than infected allogeneic target cells. cd T-cell clones isolated from this line were found to express Vc9Vd2 T-cell receptor ( TCR) and also killed infected mismatched cells more eÃciently than autologous targets. Moreover, from experiments using major histocompatability complex (MHC )-deficient cell lines, it was apparent that target cell recognition was MHC independent. Our results suggest that cd T cells can be involved in immunity to Yersinia enterocolitica and should be taken into account when considering immunopathological mechanisms leading to reactive arthritis.

Role of Monocyte Chemotactic Protein-1/CC Chemokine Ligand 2 on γδ T Lymphocyte Trafficking during Inflammation Induced by Lipopolysaccharide orMycobacterium bovisBacille Calmette-Guérin

“…They increase in numbers during the course of infection with a wild array of pathogens, including bacteria, virus, and parasites (52)(53)(54). The reactivity of ␥␦ T lymphocytes to mycobacteria has attracted considerable attention to the involvement of this T subset in the pathophysiology of tuberculosis.…”

The Recognition of HLA-B27 by Human CD4+ T Lymphocytes

“…In addition, 220 transfected with HLA-B8 was used. EBV LCLs were produced from PBMC as previously described (17).…”

Antigen Recognition by Human γδ T Lymphocytes

“…Early in vitro experiments indicated that the dominant subset of peripheral blood γδ T cells, i.e. Vγ9/Vδ2-expressing cells, strongly reacted in a non-MHC-restricted fashion towards inactivated Mycobacterium tuberculosis [17, 18]and a variety of other microorganisms, including Plasmodium falciparum [19], Toxoplasma gondii [20], Yersinia enterocolitica [21] , Francisella tularensis [22]and others. Further investigations revealed that the γδ T cell-stimulating moiety of microbial extracts was not protein, but rather consisted of nonproteinaceous, phosphatase-sensitive, low-molecular-weight compounds [23].…”