Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+CD27+CD43+CD70−

Griffin, Daniel O.; Holodick, Nichol E.; Rothstein, Thomas L.

doi:10.1084/jem.20101499

Cited by 533 publications

(461 citation statements)

References 78 publications

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“…In mouse models, it was shown that IgM secreted by B1 B cells was responsible for their atheroprotective effect 28. In contrast to their role in mouse studies,28, 33 CD43 + B cells, suggested to resemble B1 cells in mice,15 did not associate with protection against secondary events in our study. Contrasting results have been reported for the association of serum IgM levels with primary cardiovascular events 51, 52, 53.…”

Section: Discussioncontrasting

confidence: 93%

“…Analysis of the flow cytometry data was performed using Kaluza 1.3 software. We selected viable CD19 + CD3 − lymphocytes, excluded plasmablasts (CD24 − CD38 + ; Figure 1), and gated CD43 + CD27 + cells, which are suggested to resemble B1 B cells 15. Next, we selected unswitched memory cells (CD27 + CD43 − IgD + ) and switched memory cells (CD27 + CD43 − IgD − ).…”

Section: Methodsmentioning

confidence: 99%

“…The total peripheral B‐cell pool in humans mainly consists of naïve B cells, CD43 + B1‐like cells,15, 16, 17 unswitched and switched memory cells (mainly expressing immunoglobulin [Ig] M,18 or IgG and IgA antibodies, respectively18), regulatory B cells, and plasma cells. As such, B cells are important in immune homeostasis, but have also been shown to be detrimental in autoimmune diseases like common variable immunodeficiency and systemic lupus erythematosus 19, 20.…”

Section: Introductionmentioning

confidence: 99%

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High Levels of (Un)Switched Memory B Cells Are Associated With Better Outcome in Patients With Advanced Atherosclerotic Disease

Meeuwsen

Duijvenvoorde

Gohar

et al. 2017

JAHA

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BackgroundAtherosclerosis is an inflammatory lipid disorder and the main underlying pathology of acute ischemic events. Despite a vast amount of data from murine atherosclerosis models, evidence of B‐cell involvement in human atherosclerotic disease is limited. We therefore investigated the association of circulating B‐cell subtypes with the occurrence of secondary cardiovascular events in advanced atherosclerotic disease.Methods and ResultsThis cohort study consists of 168 patients who were included in the Athero‐Express biobank between 2009 and 2011. Before surgery, peripheral blood mononuclear cells were isolated and stored in liquid nitrogen. After gentle thawing of the peripheral blood mononuclear cells, different B‐cell subtypes including naïve, (un)switched memory, and CD27+ CD43+ B1‐like B cells, were analyzed by flow cytometry. Univariable and multivariable Cox proportional hazard models were used to analyze associations between B‐cell subtypes, circulating antibodies and secondary cardiovascular manifestations during the 3‐year follow‐up period. Mean age was 70.1±9.6 years, males represented 62.8% of the population, and 54 patients had secondary manifestations during follow‐up. High numbers of unswitched memory cells were protective against secondary outcome (hazard ratio, 0.30 [95% CI, 0.13–0.69]; P<0.01). Similar results were obtained for the switched memory cells that also showed to be protective against secondary outcome (hazard ratio, 0.33 [95% CI, 0.14–0.77]; P=0.01).ConclusionsA high number of (un)switched memory B cells is associated with better outcome following carotid artery endarterectomy. These findings suggest a potential role for B‐cell subsets in prediction and prevention of secondary cardiovascular events in patients with atherosclerosis.

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Section: Discussioncontrasting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High Levels of (Un)Switched Memory B Cells Are Associated With Better Outcome in Patients With Advanced Atherosclerotic Disease

Meeuwsen

Duijvenvoorde

Gohar

et al. 2017

JAHA

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“…Recently, Griffin et al (5) claimed the discovery of a small subset of human CD20 ϩ B cells expressing CD27 and CD43 that recap the functional characteristics of murine B-1 cells. However, controversy has arisen about the nature of these cells, and we recently showed that CD27 ϩ CD43 ϩ B cells are in fact activated cells on their way to plasma cell differentiation (6)(7)(8)(9).…”

mentioning

confidence: 99%

Anti-Pneumococcal Capsular Polysaccharide Antibody Response and CD5 B Lymphocyte Subsets

et al. 2015

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“…Each experimental replicate was performed independently with primary human CD34 ϩ cells from different donors. CD34 ϩ cells were isolated with magnetic beads from cord blood samples obtained from the Long Island Blood Service or obtained preisolated from AllCells (11,12). CD34 ϩ cells were put into culture in serum-free VIVO-X 20 medium.…”

mentioning

confidence: 99%

HIV-1 Is Restricted prior to Integration of Viral DNA in Primary Cord-Derived Human CD34 ⁺ Cells

Griffin

Goff

2015

J Virol

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There is currently significant controversy regarding whether human hematopoietic stem cells (HSCs) can become infected with HIV-1; some but not all investigators have detected HIV-1 in human HSCs of infected patients (1, 2). Lentiviruses do successfully infect nondividing cells, but HIV-1 infection of human HSCs in culture is inefficient compared to that in activated T cells (3)(4)(5). A significant body of work has identified postintegration silencing as a major mechanism employed by murine stem cells (MSCs) to restrict retroviruses, but human HSCs may be different. The point in the retroviral replication cycle at which retroviruses are blocked in human HSCs remains unknown, and whether this involves a preintegration block or is due to transcriptional silencing of integrated proviruses has not been studied in detail (6-10).This investigation evaluated infection of primary cord-derived human CD34 ϩ cells, a mixed population composed of true human HSCs (Lin Ϫ 34 ϩ 38 Ϫ 90 ϩ 45RA dim ) as well as several hematopoietic progenitor cell (HPC) types. We identified a block occurring prior to viral DNA integration as a major factor in the nonpermissiveness of cord-derived primary CD34 ϩ cells for HIV-1 infection. Transduction of primary cord-derived CD34؉ cells by lentiviral vectors is inefficient at low MOIs. Primary human cordderived CD34 ϩ cells were used to study the efficiency of lentiviral transduction under various conditions. Each experimental replicate was performed independently with primary human CD34 ϩ cells from different donors. CD34 ϩ cells were isolated with magnetic beads from cord blood samples obtained from the Long Island Blood Service or obtained preisolated from AllCells (11,12). CD34 ϩ cells were put into culture in serum-free VIVO-X 20 medium. These cells were either cultured in the absence of cytokines or prestimulated for 24 h with various cytokines, singly or in combination, prior to exposure to preparations of pseudotyped lentiviral vectors. Vesicular stomatitis virus G (VSV-G)-pseudotyped virus with a modified pNL4.3 HIV-1-based core was prepared by cotransfecting 293T cells with a pNL4.3.mCherry.RϪ .E Ϫ HIV-1-based plasmid lacking env and vpr genes and having an mCherry open reading frame (ORF) instead of the nef gene, together with a VSV-G expression plasmid (13). We used a VSV-G envelope to bypass cell entry limitations and to look specifically at postentry events. Multiplicities of infection (MOIs) were calculated based on viral titers of mCherry transduction units, determined by infection of the permissive 293T cell line with serial dilutions of the virus preparation. The cells were preincubated for 24 h with no cytokines, Flt3 ligand (FLT3L) at 100 ng/ml, stem cell factor (SCF) at 100 ng/ml, thrombopoietin (TPO) at 100 ng/ml, or all three cytokines at 100 ng/ml. These cells were then exposed to pseudotyped virus by spinoculation at 37°C and 2,000 rpm for 60 min. Cells were then left in culture for 72 h at 37°C and evaluated using flow cytometry and PCR. All experiments were performed i...

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Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+CD27+CD43+CD70−

Abstract: Human B1 cells consist of CD20+CD27+CD43+CD70− cells bearing a skewed B cell receptor repertoire, and are present in umbilical cord and adult peripheral blood.

Cited by 533 publications

References 78 publications

High Levels of (Un)Switched Memory B Cells Are Associated With Better Outcome in Patients With Advanced Atherosclerotic Disease

High Levels of (Un)Switched Memory B Cells Are Associated With Better Outcome in Patients With Advanced Atherosclerotic Disease

Anti-Pneumococcal Capsular Polysaccharide Antibody Response and CD5 B Lymphocyte Subsets

HIV-1 Is Restricted prior to Integration of Viral DNA in Primary Cord-Derived Human CD34 ⁺ Cells

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Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+CD27+CD43+CD70−

Abstract: Human B1 cells consist of CD20+CD27+CD43+CD70− cells bearing a skewed B cell receptor repertoire, and are present in umbilical cord and adult peripheral blood.

Cited by 533 publications

References 78 publications

High Levels of (Un)Switched Memory B Cells Are Associated With Better Outcome in Patients With Advanced Atherosclerotic Disease

High Levels of (Un)Switched Memory B Cells Are Associated With Better Outcome in Patients With Advanced Atherosclerotic Disease

Anti-Pneumococcal Capsular Polysaccharide Antibody Response and CD5 B Lymphocyte Subsets

HIV-1 Is Restricted prior to Integration of Viral DNA in Primary Cord-Derived Human CD34 + Cells

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HIV-1 Is Restricted prior to Integration of Viral DNA in Primary Cord-Derived Human CD34 ⁺ Cells