Human “barcode”: Link between phosphate intensity changes in human enamel and light microscopy record of accentuated lines

Vacková, Soňa; Králík, Miroslav; Marečková, Klára; Ráčková, Lucie; Quade, Leslie; Sedlačková, Lenka; Fojtík, Pavel; Kučera, Lukáš

doi:10.1016/j.microc.2021.106370

Cited by 8 publications

(4 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results from histological examination of archaeozoological specimens are published in Boriová et al (2020); Sazelová et al (2020aSazelová et al ( , 2020b, and are not part of this study. Histological examination results of chosen anthropological specimens are available in Vacková et al (2021) and in unpublished thesis by Pelikán (2018).…”

Section: Sample Compositionmentioning

confidence: 99%

“…200x or 400x for examination of smaller bone microstructures such as lacunae and measurement of daily increments in dental enamel. Application of the specific microscopic analysis of Late Pleistocene specimens was published inSazelová et al (2020a,b), with part of the anthropological samples microscopic analysis availableVacková et al (2021) and in the thesis byPelikán (2018).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Preparation of thin histological sections from archaeological bone and tooth samples

Vacková¹,

Boriová²,

Sázelová³

et al. 2022

Self Cite

View full text Add to dashboard Cite

Histological analysis of osteological remains from archaeological excavations provides data and information that can be difficult or impossible to obtain from macroscopic description and examination. Furthermore, the microscopic perspective provides important evidence for taxonomically indeterminate samples lacking morphologically diagnostic marks, e.g. determination of human/non-human bone origin, and can provide further information about analysed individual, e.g. studying of developmental stress in dental enamel. Microscopy of bone and tooth samples requires preparation of good quality thin histological sections for transmitted and polarized light microscopy examination and analysis. This paper presents detailed methodology description in several main steps which were modified to fit our laboratory. Finally, we suggest an easily repeatable protocol and know-how fitting in similar or identical laboratory conditions, including equipment, consumables, and other items

show abstract

Section: Sample Compositionmentioning

confidence: 99%

mentioning

confidence: 99%

Preparation of thin histological sections from archaeological bone and tooth samples

Vacková¹,

Boriová²,

Sázelová³

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ablative techniques such as LA-ICP-MS or SIMS primarily sample phosphate (Cerling and Sharp, 1996;Smith et al, 2018)-the dominant O-bearing species in bioapatite-and we routinely observe that developing (hypomineralized) enamel produces lower δ 18 O values due to its higher organic content. Thus, δ 18 O may be expected to decrease in accentuated lines, such as the neonatal line, due to reduced Ca-phosphate in these hypomineralized areas (Austin et al, 2016;Dean et al, 2019;Vacková et al, 2021). Once neonates begin to respire and lose water transcutaneously, initial δ 18 O values in postnatal enamel should increase due to the preferential loss of the light isotope ( 16 O) from body water (Bryant et al, 1996;Kohn and Cerling, 2002).…”

Section: Oxygen Isotopic Changes At Birthmentioning

confidence: 99%

Permanent Signatures of Birth and Nursing Initiation are Chemically Recorded in Teeth

et al. 2021

View full text Add to dashboard Cite

“…Moreover, the identification of stress markers, such as Accentuated Lines (ALs, more pronounced striae of Retzius) and the Neonatal Line (NNL, an AL that forms at birth), allows us to identify respectively the occurrence of physiological stressors during dental development and the birth event [9,[37][38][39][40][41][42][43][44][45]. These latter lines act as permanent records of physiological stress events above a certain thresholdincluding birth, illnesses, and nutritional deficiencieswhich are permanently etched within the enamel's microstructure during its formation [11,[46][47][48][49].…”

Section: Introductionmentioning

confidence: 99%

Enamel histomorphometry, growth patterns and developmental trajectories of the first deciduous molar in an Italian early medieval skeletal series

Magri,

Higgins,

Lugli

et al. 2024

Preprint

View full text Add to dashboard Cite

Understanding the growth patterns and developmental trajectories of teeth during early life stages provides valuable insights into the ontogeny of individuals, particularly in archaeological populations where such information is scarce. This study focuses on first deciduous molars, specifically investigating crown formation times and daily secretion rates, through histological analysis. A total of 34 teeth from the Early Medieval necropolises of Casalmoro and Guidizzolo (Mantua, Lombardy, northern Italy) were analysed assessing growth parameters and identifying possible differences between sites and between sexes, which are determined through proteomic analysis. Furthermore, a robust linear regression model relating prism length and secretion time was built to estimate growth rates also in teeth in which the finest incremental markings are not visible. The daily secretion rates (DSR) in inner enamel showed a high homogeneity between dental arches, sexes and the two sites. Values fall within the known range reported in the literature for the same tooth class in archaeological populations. However, a difference in DSR was observed when compared with modern sample published values. Crown formation times and age at crown completion is different between dental arches, with maxillary first molars initiating their matrix apposition earlier than mandibular molars as formerly reported. However, no significant differences were highlighted in association with sex. This study expands our understanding of the growth and development of first deciduous molars in a medieval population, providing valuable insights into growth trajectories specific to the dental arch. These findings highlight the need for extensive investigations using similar methodologies to attain more accurate and comprehensive information about the developmental patterns of first deciduous molars and underscore the potential use of proteomic analysis to explore deciduous teeth microstructure even in archaeological populations.

show abstract

Human “barcode”: Link between phosphate intensity changes in human enamel and light microscopy record of accentuated lines

Cited by 8 publications

References 47 publications

Preparation of thin histological sections from archaeological bone and tooth samples

Preparation of thin histological sections from archaeological bone and tooth samples

Permanent Signatures of Birth and Nursing Initiation are Chemically Recorded in Teeth

Enamel histomorphometry, growth patterns and developmental trajectories of the first deciduous molar in an Italian early medieval skeletal series

Contact Info

Product

Resources

About