Human blastocyst morphological quality is significantly improved in embryos classified as fast on day 3 (≥10 cells), bringing into question current embryological dogma

Luna, M.; Copperman, A.B.; Duke, M.; Ezcurra, D.; Sandler, B.; Barritt, J.

doi:10.1016/j.fertnstert.2007.03.030

Cited by 58 publications

(37 citation statements)

References 23 publications

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“…However, there was not significant difference in embryo development to blastocyst when day three embryos had 8-10 or more cells (Table 2), which reaffirms that fast cleaving embryos are as good as embryos with 8 cells to accomplish the blastocyst stage. This result is similar to that reported by Luna (Luna et al, 2008), and reaffirm that fast cleavage at day three is not a poor prognostic factor for the embryo. On the other hand, as was also suggested by Shapiro (Shapiro et al, 2000), the probability of reaching a good quality blastocyst from an embryo with reach day five being an expanded blastocyst.…”

Section: Resultssupporting

confidence: 91%

“…Many parameters have been used to predict the outcome of cleaving embryos, such as the speed of cleavage (Luna et al, 2008), number of blastomeres (Shapiro et al, 2000;Langley et al, 2001;Luna et al, 2008) and embryo morphology at day 3 (Rijnders & Jansen 1998;Stone et al, 2005;nomura et al, 2007), all of which, provide relevant elements to evaluate the capacity of cleaving embryos to reach blastocyst. This study combines all parameters, allowing a comparison between them, and also provides novel information about the characteristics of developing embryos when blastocysts have 100% implantation.…”

Section: Discussionmentioning

confidence: 99%

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Embryo early cleavage, number of blastomeres and morphology at day three as factors to predict blastocyst development

Mackenna¹,

Crosby²,

Zegers‐Hochschild³

2013

JBRA Assisted Reproduction

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Copyright -Todos os direitos reservados a SBRA -Sociedade Brasileira de Reprodução AssistidaEmbryo early cleavage, number of blastomeres and morphology at day three as factors to predict blastocyst development Clivagem precoce do embrião, número de blastocistos e morfologia embrionária no dia 3 como fatores preditivos para o desenvolvimento até blastocisto ABSTRACTObjective: the objective of this study is to evaluate markers of embryo quality, applied on day three of in vitro culture, in their capacity to predict whether embryos will reach blastocyst stage. Methods: a retrospective analysis of embryology database coming from 2772 zygotes obtained in 564 oocyte retrievals was performed; embryos were observed from day one to five: 23 to 26 hours after insemination all zygotes were evaluated to assess early cleavage, the number of blastomeres and embryo morphology were registered on day three and blastocyst formation was evaluated on day five; early first cleavage, number of blastomeres and embryo morphology at day three were correlated with embryo development until day five. Results: blastocyst formation rate was 36% in embryos with early cleavage and 19% in embryos without early the probability of a good quality embryo (grade 1 and 2a) at day three to reach the blastocyst stage was 48%, which was significantly higher than the chances of lower quality embryos. Conclusion: factors reported in this study -early cleavage, number of blastomeres and embryo morphology -are key words: blastocyst, expanded blastocyst, early cleavage, blastomere number, embryo morphology. RESUMENObjetivo: el objetivo del presente trabajo es evaluar los marcadores de calidad embrionaria, observados el tercer día de cultivo, para predecir el desarrollo del embrión hasta blastocisto. Métodos: se realizó un análisis retrospectivo de la base de datos del Laboratorio de Embriología, que proviene de 2772 cigotos obtenidos de 564 aspiraciones foliculares; los embriones fueron evaluados desde el día uno al quinto de desarrollo, registrando lo siguiente, 23 a 26 horas después de la inseminación, todos los cigotos fueron evaluados para determinar el clivaje temprano; el día 3 de desarrollo se evaluó el número de blastómeros y morfología embrionaria; el día quinto se evaluó la formación de un blastocisto, se correlacionó el clivaje temprano, el número de blastomeros y la morfología embrionariaa con el desarrollo embrionario hasta el día quinto. Resultados: la tasa de blastulación fue 36% en embriones con clivaje temprano y 19% en los embriones sin -día 3, la probabilidad de los embriones de buena calidad (grado 1 y 2a) en día 3 de desarrollo de alcanzar el estadío de blastocisto fue de 48%, que fue significativamente mejor que la probabilidad de los embriones de peor morfología. Conclusión: los factores reportados en este estudioclivaje temprano, número de blastomeros y morfología embrionaria -son útiles para decidir el cultivo prolongado in vitro hasta día quinto de desarrollo embrionario.

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Section: Resultssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Embryo early cleavage, number of blastomeres and morphology at day three as factors to predict blastocyst development

Mackenna¹,

Crosby²,

Zegers‐Hochschild³

2013

JBRA Assisted Reproduction

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“…It was also found that there was no significant difference in the rates of formation of blastocysts and good-morphology blastocysts from high-quality embryos of 6-8 cells and !9 cells. These findings are consistent with an earlier study, comparing embryos of 6 cells, 7-9 cells, and !10 cells, which observed that the rates of formation of blastocysts and goodquality blastocysts were significantly higher in rapid-cleavage embryos than in slow-cleavage embryos [Luna et al 2008]. Those results suggested that the rate of blastocyst formation was closely related to day-3-embryo morphology and cell number.…”

Section: Discussionmentioning

confidence: 84%

The effects of fertilization mode, embryo morphology at day 3, and female age on blastocyst formation and the clinical outcomes

Yin

Jiang

et al. 2014

Systems Biology in Reproductive Medicine

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(2015) The effects of fertilization mode, embryo morphology at day 3, and female age on blastocyst formation and the clinical outcomes, Systems Biology in Reproductive Medicine, 61:1, 50-56, DOI: 10.3109/19396368.2014 AbstractThe aim of this study was to evaluate the influence of in vitro fertilization (IVF) versus intracytoplasmic sperm injection (ICSI), fertilization mode embryonic morphology at day 3, and female age on blastocyst development, on the clinical outcomes of pregnancy after blastocyst transfer. A total of 471 cycles were retrospectively investigated. The rates of blastocyst formation and of good blastocyst morphology were higher in IVF than in ICSI cycles but there were no significant differences in the clinical pregnancies or in the miscarriage rates. The rates of formation of blastocyst and of blastocysts with good morphology were significantly higher from good-morphology embryos than from poor-morphology embryos. Nevertheless, 16.9% of the poor-morphology embryos reached the blastocyst stage. The total rates of blastocyst formation, and rates of clinical pregnancy and implantation were statistically similar in the age 535, 35-39, and 439 year groups, although tending to decrease with increasing age. When equal numbers of embryos were transferred on day 3, the rates of clinical pregnancy and implantation after blastocyst transfer were significantly higher in the 535 year age group than in the 35-39 and 439 year age groups, which were not significantly different. The miscarriage rates after embryo or blastocyst transfers were not statistically different in groups of similar age. Therefore, extended embryo culture up to the blastocyst stage could be implemented for women aged younger than 35 years to increase the pregnancy rate. For older women, transfer and vitrification of available embryos at day 3 and extended culture of morphologically poor embryos to the blastocyst stage for cryopreservation may improve the clinical outcome.

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“…[29][30][31] Others reported that blastocysts with greater numbers of TE cells develop a more robust placenta in utero, improving sustenance of the fetus. 26,27 It is possible that the increased number of hatching/hatched blastocysts in the present study was a result of a significantly higher number of TE nuclei in the single medium, leading to a higher rate of blastocyst expansion. In contrast to single medium, the early stage medium of the sequential culture does not contain amino acids, glucose, and EDTA (Table 1).…”

Section: Discussionmentioning

confidence: 84%

Improved Murine Blastocyst Quality and Development in a Single Culture Medium Compared to Sequential Culture Media

Hennings¹,

Zimmer²,

Nabli³

et al. 2016

Reprod. Sci.

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Objective: Validate single versus sequential culture media for murine embryo development. Design: Prospective laboratory experiment. Setting: Assisted Reproduction Laboratory. Animals: Murine embryos. Interventions: Thawed murine zygotes cultured for 3 or 5 days (d3 or d5) in single or sequential embryo culture media developed for human in vitro fertilization. Main Outcome Measures: On d3, zygotes developing to the 8 cell (8C) stage or greater were quantified using 4',6-diamidino-2-phenylindole (DAPI), and quality was assessed by morphological analysis. On d5, the number of embryos reaching the blastocyst stage was counted. DAPI was used to quantify total nuclei and inner cell mass nuclei. Localization of ubiquitin C-terminal hydrolase L1 (UCHL1) and ubiquitin C-terminal hydrolase L3 (UCHL3) was reference points for evaluating cell quality. Results: Comparing outcomes in single versus to sequential media, the odds of embryos developing to the 8C stage on d3 were 2.34 time greater (P ¼ .06). On d5, more embryos reached the blastocyst stage (P ¼ <.0001), hatched, and had significantly more trophoblast cells (P ¼ .005) contributing to the increased total cell number. Also at d5, localization of distinct cytoplasmic UCHL1 and nuclear UCHL3 was found in high-quality hatching blastocysts. Localization of UCHL1 and UCHL3 was diffuse and inappropriately dispersed throughout the cytoplasm in low-quality nonhatching blastocysts. Conclusions: Single medium yields greater cell numbers, an increased growth rate, and more hatching of murine embryos. Cytoplasmic UCHL1 and nuclear UHCL3 localization patterns were indicative of embryo quality. Our conclusions are limited to murine embryos but one might speculate that single medium may also be more beneficial for human embryo culture. Human embryo studies are needed.

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Human blastocyst morphological quality is significantly improved in embryos classified as fast on day 3 (≥10 cells), bringing into question current embryological dogma

Cited by 58 publications

References 23 publications

Embryo early cleavage, number of blastomeres and morphology at day three as factors to predict blastocyst development

Embryo early cleavage, number of blastomeres and morphology at day three as factors to predict blastocyst development

The effects of fertilization mode, embryo morphology at day 3, and female age on blastocyst formation and the clinical outcomes

Improved Murine Blastocyst Quality and Development in a Single Culture Medium Compared to Sequential Culture Media

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