Human Blood Plasma Proteins

Schaller, Johann; Gerber, Simon; Kmpfer, Urs; Lejon, Sofia; Trachsel, Christian

doi:10.1002/9780470724378

Cited by 94 publications

(99 citation statements)

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“…The larger proteins (Table 1), [22] which have larger amount of binding sites to interact with the materials' surface, have higher potential to be adsorbed on the surface. However, for a complex solution, such as blood plasma, which contains hundreds of different proteins, [23] the mass transfer rate of protein molecules to the surface is directly related to its concentration and inversely to its molecular weight. Therefore, for a multiprotein system, small proteins of higher concentration and higher diffusion rate tend to adsorb onto the surface first, and then are displaced by larger molecules.…”

Section: Adsorption Influenced By Protein Molecular Propertiesmentioning

confidence: 99%

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Firkowska‐Boden

Zhang

Jandt

2017

Adv Healthcare Materials

105

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The initial host response to healthcare materials' surfaces after implantation is the adsorption of proteins from blood and interstitial fluids. This adsorbed protein layer modulates the biological/cellular responses to healthcare materials. This stresses the significance of the surface protein assembly for the biocompatibility and functionality of biomaterials and necessitates a profound fundamental understanding of the capability to control protein–surface interactions. This review, therefore, addresses this by systematically analyzing and discussing strategies to control protein adsorption on polymeric healthcare materials through the introduction of specific surface nanostructures. Relevant proteins, healthcare materials' surface properties, clinical applications of polymer healthcare materials, fabrication methods for nanostructured polymer surfaces, amorphous, semicrystalline and block copolymers are considered with a special emphasis on the topographical control of protein adsorption. The review shows that nanostructured polymer surfaces are powerful tools to control the amount, orientation, and order of adsorbed protein layers. It also shows that the understanding of the biological responses to such ordered protein adsorption is still in its infancy, yet it has immense potential for future healthcare materials. The review, which is—as far as it is known—the first one discussing protein adsorption on nanostructured polymer surfaces, concludes with highlighting important current research questions.

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Section: Adsorption Influenced By Protein Molecular Propertiesmentioning

confidence: 99%

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Firkowska‐Boden

Zhang

Jandt

2017

Adv Healthcare Materials

105

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“…2) has strong spectral features of two proteins: hemoglobin and fibrin, which are the main building blocks of red blood cells and plasma, respectively. 69 The presence of two spectral components dominated by these proteins was also revealed by advanced statistical analysis of Raman spectra recorded from multiple spots of a single dry sample of blood ( Figure 3A). 70,71 Component 1 has characteristic peaks of hemoglobin and its derivatives at 1000, 1368, 1542, and 1620 cm -1 (Fig.…”

Section: -43mentioning

confidence: 80%

“…39,69 The peaks at 967, 1248, and 1342 cm -1 of component 2 were attributed to pure fibrin, one of the components of coagulated blood. 72 The amide III vibrational mode of the protein backbone, which consists of C-N stretching, N-H bending, and C-C stretching, 42,69 is evident at 1247 cm -1 . This assignment was also supported by observation of the coagulation process when blood dries.…”

Section: -43mentioning

confidence: 99%

Multidimensional Raman Spectroscopic Signatures as a Tool for Forensic Identification of Body Fluid Traces: A Review

2011

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The analysis of body fluid traces during forensic investigations is a critical step in determining the key details of a crime. Several confirmatory and presumptive biochemical tests are currently utilized. However, these tests are all destructive, and no single method can be used to analyze all body fluids. This review outlines recent progress in the development of a novel universal approach for the nondestructive, confirmatory identification of body fluid traces using Raman spectroscopy. The method is based on the use of multidimensional spectroscopic signatures of body fluids and accounts for the intrinsic heterogeneity of dry traces and donor variation. The results presented here demonstrate that Raman spectroscopy has potential for identifying traces of semen, blood, saliva, sweat, and vaginal fluid with high confidence.

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“…We observed some cross-reactivity, however, this should have no impact on the study because the circulating half-life of cytokines is usually minutes to hours, although it differs for individual cytokines [24]. In this study, the serum was collected from mice 6 days after the last IV administration of human secretions.…”

Section: Data Analysis Of Bio-plex Datasetsmentioning

confidence: 95%

“…A reduction of the clinical score to baseline at day 12 was observed in the CAIA mice treated with the SVF adipocyte co-culture treatment group. If it is assumed the circulating half-life of the human cytokines administered to the CAIA mice was minutes to hours [24], this reduction of the clinical score occurred 6 days after the human cytokines were cleared from the circulation. One of the mechanisms of action of MSCs is their ability to induce peripheral T cell tolerance by suppressing T cell proliferation and inducing T regulatory cell production [32,34].…”

Section: Svf Adipocyte Co-culture Secretions: a Promising Therapeuticmentioning

confidence: 99%

Treatment of a mouse model of collagen antibody-induced arthritis with human adipose-derived secretions

Blaber¹,

Webster²,

Breen³

et al. 2013

OJRM

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The use of adipose-derived cells as a treatment for a variety of diseases is becoming increasingly common. These therapies include the use of cultured mesenchymal stem cells (MSCs) and freshly isolated stromal vascular fraction (SVF) alone, or in conjunction with other cells such as adipocytes. There is a substantial amount of literature published on the therapeutic properties of MSCs and their secretions as the main driver of their therapeutic effect. However, there is little data available on the therapeutic potential of secretions from SVF, either with or without adipocytes. We investigated the ability of secretions from human adipose SVF alone and the SVF co-cultured with adipocytes as a proxy for cell therapy, to ameliorate an inflammatory disorder. This ethics approved study involved the treatment of collagen antibody-induced arthritis (CAIA) in mice with secretions from SVF, SVF co-cultured with adipocytes, or a vehicle control via both intravenous (IV) and intramuscular (IM) routes. Treatment outcome was assessed by paw volume, ankle size and clinical arthritis score measurements. Serum samples were obtained following euthanasia and analysed for a panel of 32 mouse cytokines and growth factors. The dose and timing regime used for the IM administration of both human secretion mixtures did not significantly ameliorate arthritis in this model. The IV administration of SVF adipocyte co-culture secretions reduced the paw volume, and significantly reduced the ankle size and clinical arthritis score when compared to the IV vehicle control mice. This was a superior therapeutic effect than treatment with SVF secretions. Furthermore, treatment with SVF adipocyte coculture secretions resulted in a significant reduction in serum levels of key cytokines, IL-2 and VEGF, involved in the pathogenesis of rheumatoid arthritis. Therefore, the SVF cocultured with adipocytes is an attractive therapeutic for inflammatory conditions.

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Human Blood Plasma Proteins

Cited by 94 publications

References 0 publications

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Multidimensional Raman Spectroscopic Signatures as a Tool for Forensic Identification of Body Fluid Traces: A Review

Treatment of a mouse model of collagen antibody-induced arthritis with human adipose-derived secretions

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