Human blood platelets contract in perpendicular direction to shear flow

Abstract

We study the spreading and contraction behavior of platelets in microfluidic flow.

The Influence of Substrate Elasticity and Shear Rate on Human Blood Platelet Contraction

“…The difference in the maximal velocity between the simulated and measured velocity is less than 5 %. The image is taken from Hanke et al [37].…”

“…Let us now take a look at the displacement we observe in the substrate. As our cell is modelled as a circular disc of isotropic contraction, in mathematical terms, the problem is axially symmetric and the strain tensor is given in spherical coordinates as 37) with u being the displacement as before. Note that due to the condition of isotropy, the angle does not play a role in this equation.…”

“…The PDMS stripe used to protect the PAA substrate is removed beforehand. The sketch is adapted from Hanke et al [37].…”

“…The high peaks in the graph denote the positions of, from left to right, the inlet, the first gel edge, the second gel edge and the outlet. The linear scaling of the shear rate with the flow rate is illustrated when comparing the values above the substrate for C and D. Note that panel C was adapted from Hanke et al [37]. flow rate utilised during the measurements, the shear rates directly above the gel are shown in Fig.…”

“…The approximate positions of the highest and lowest von Mises stress are included by the dark red and dark blue arcs, respectively. The figure is adapted from Hanke et al [37]. similar experiments were conducted using layers of already attached endothelial cells [47,57,92].…”