Human bocavirus (HBoV) in children with respiratory tract infection by enzyme linked immunosorbent assay (ELISA) and qualitative polymerase chain reaction (PCR)

Abstract

BackgroundHuman bocavirus (HBoV) is a recently discovered parvovirus associated with mild to severe lower respiratory tract infections in children, the aim of the work was determination of human bocavirus in nasopharyngeal aspirate (NPA) of infants by qualitative PCR and determination of acute human bocavirus infection by estimation of immunoglobulin M (IgM) antibodies in serum by enzyme linked immunosorbent assay.ResultsTwenty two (22%) out of the 100 NPA specimens of the patients with respiratory manifestations were positive for HBoV by qualitative PCR, while ELISA revealed positive HBoV IgM antibodies in 18 (18%) patients who were also positive by PCR. Non of the controls were positive by both techniques. The correlation study between ELISA and PCR revealed high significant association, (p < 0.001, X2 = 36 and agreement = 96%). Also PCR detected 4 (18.1%) NPA samples as HBoV positive cases among the patients that were not identified by ELISA. This could be due to high sensitivity and efficacy of PCR. ELISA being less sensitive than RT-PCR, sensitivity was (81.8% vs 100%), the efficacy was 97.7% in ELISA versus 99.7% for RT-PCR.ConclusionHBoV infections could be diagnosed in NPA of children by conventional PCR as a rapid and sensitive technique. While ELISA was a reliable serologic analysis for diagnosis of acute HBoV infection by estimation IgM antibodies in serum.

Detection of Human Bocavirus in Egyptian Children Suffering from Acute Lower Respiratory Tract Infection

“…We estimated HBoV IgM as another diagnostic tool for diagnosis of HBoV causing ALRI and we found that there were13/ 123 (16%) positive cases by ELISA, these results were in agreement with previous results of IgM detection in children with HBoV in the studies by Zaghloul (2011) andSöderlund-Venermo et al, (2009) that were 18%, 19% respectively.…”

“…This study result was in agreement with that of Zaghloul (2011). However, our results were higher than that of Lindner et al, (2008) There was significant association between high viral load and positive serology that probably indicate active HBoV infection; there were 13IgM-positive serum samples of patients who had high viral load among 16 IgM-positive serums.…”

“…The three negative serological results had low viral load, this result was in agreement with Allander et al, (2007) who reported that seropositive cases mainly showed high viral load in nasopharyngeal aspirate. As regards to sensitivity, specificity, positive predictive value and negative predictive value of ELISA were 84.2%, 100%,100% and 97.2%,our results are near to values recorded previously by Lindner et al, (2008) and Zaghloul (2011). In conclusion, human bocavirus is an important cause of ALRI in young children less than five years; its incidence is highest in winter months.…”

“…However, our results were lower than the results of two previous studies in Egypt by Abdel-Moneim et al, (2016), Zaghloul (2011who reported that HBoV was found in 56.8% and 24.5% respectively in the examined children in their studies, also our result was lower than that reported by Denge et al, (2012) that was 22% and higher than those reported by Weissbrich et al, (2006) and Neske et al, (2007) who had found HBoV DNA by PCR techniques in NPA samples were 10.3%, 12% respectively. This variation may be due to different age groups of patients, different methods of detection of the virus.…”

Simple and Rapid Detection of Potato leafroll virus by Reverse Transcription Loop-mediated Isothermal Amplification

“…To increase sensitivity of virus detection by ELISA, sprouts from tubers have been used (Mumford et al, 2000). Conventional polymerase chain reaction (PCR) or reverse transcription-PCR (RT-PCR) which have high sensitivity and specificity over ELISA-based methods and have been used to detect DNA viruses or RNA viruses, respectively (Bostan and Peker, 2009;Stark et al, 2008;Zaghloul, 2011). RT-PCR and immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) have been employed for detection of PLRV in plant or in aphid vectors (Ahouee et al, 2010;Hadidi et al, 1993;Peiman and Xie, 2006;Singh et al, 1995;1997).…”