Human bone cell cultures: A new model for studying the mechanism of action of calcitonin

Abstract

An investigation on cell cultures obtained from temporal human bone fragments showed that they provide a suitable model for studying the mechanism involved in calcitonin action on bone cells. Furthermore they demonstrated: a transitory increase in 45Ca uptake that returned to control values ten minutes after the hormone was added; a relation between 45Ca uptake and increased cAMP concentrations when these were measured at the same time intervals; a reproduction of the salmon calcitonin (sCT) effect after incubation of the cultures with either db-cAMP or db-cGMP and inhibition of 45Ca uptake and parallel decrease in cAMP levels with propanol. These results suggest that in human bone cell cultures, sCT acts as a temporary promoter of 45Ca uptake, probably by activating an adenylate-cyclase system through a beta-receptor.

Characterization of the Cytoskeleton in Human Normal and Otosclerotic Osteoblast-Like Cells

“…The present study was carried out in normal and otosclerotic osteoblast-like cells characterized biochemically and morphologically in previous investigations [1][2][3]. Light-, electron-and scanning-microscopic studies of this cell sys tem in primary cultures revealed fourcell populations [4], The first was made up of osteoblast-like cells with welldeveloped cell bodies and numerous cytoplasmic pro longations.…”