Human brain in tissue culture. IV. Morphological characteristics

Rorke, Lucy B.; Gilden, Donald H.; Wróblewska, Zofia; Santoli, Daniela

doi:10.1002/cne.901610305

Cited by 36 publications

(9 citation statements)

References 12 publications

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“…The loss of a heterogeneous mix of cells at early passages, replaced at later passages by a homogeneous population of cells is one characteristic described by many researchers Rorke et al, 1975;Rutka et al, 1986;Wroblewska et al, 1975). Previous evidence suggests that adult human microglia and astrocytes have a very low basal proliferation rate (Lue et al, 2001;Walker et al, 1995;Williams et al, 1992;Yong et al, 1991).…”

Section: Discussionmentioning

confidence: 96%

“…From the late 1960s mixed dissociated or explant cultures were established from biopsy or short delay post-mortem human tissue and were successfully cultured for successive passages Ponten and Macintyre, 1968;Ponten et al, 1969;Rorke et al, 1975;Wroblewska et al, 1975). The morphology and other characteristics described by these authors (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Cellular composition of human glial cultures from adult biopsy brain tissue

Gibbons

Hughes

Roon-Mom

et al. 2007

Journal of Neuroscience Methods

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Cellular composition of human glial cultures from adult biopsy brain tissue

Gibbons

Hughes

Roon-Mom

et al. 2007

Journal of Neuroscience Methods

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“…A prominent fea-ture of the cultures was the presence of large numbers of bipolar, tripolar and multipolar GFAP positive process bearing astroglia. While other investigators have previously indicated a range of astroglial subtypes present in cultures from adult human brain [9,12,18,25,27,29,49], no consistent system for categorizing these cells or nomenclature for their description has been developed.…”

Section: Discussionmentioning

confidence: 99%

“…Cultures established from adult human cerebral cortex display heterogeneous cell populations with respect to cell shape and size [9,12,18,25,27,29,49]. Newcombe et al [25] showed that the cellular composition of primary cultures evolves with time in culture.…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity of astroglia cultured from adult human temporal lobe

Davies

Niesman

Boop

et al. 2000

Intl J of Devlp Neuroscience

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This study characterized the morphological and electrophysiological diversity of astroglia cultured from adult human cerebral temporal lobe, and explored the influence of the cytokine interleukin-1beta on these cells. The cultures contained astroglia positive for glial fibrillary acidic protein which were flat, bipolar or multipolar in shape and variable in size. A subpopulation of the bipolar and multipolar cells was positive for S100 protein. The most striking feature of these cultures was the presence of glia with long (600 micrometer) processes with few branches or only terminal branches. Patch clamp recordings of the non-stellate process bearing cells revealed prominent inward Na(+) and transient and sustained outward K(+) conductances. Distinct differences in the relative proportion of these conductances were evident among cells but did not appear to be correlated with cell morphology. Treatment of cultures with interleukin-1beta for 96 h did not change total protein content, but increased the content of S100beta protein and decreased the content of glial fibrillary acidic protein. The findings indicate that cultures of adult human cerebrum contain subpopulations of morphologically and electrophysiologically pleomorphic glial fibrillary acidic protein positive astroglia, exhibit increased levels of the neurotrophic factor S100beta when exposed to interleukin-1beta, and may serve as a useful model for investigation of glial involvement in neuropathology.

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“…Oligodendrocytes isolated in bulk from adult normal rat or human brain can also be maintained in culture (Kim, McMorris & Sprinkle, 1984;Kim, 1985). Although glial fibrillary acidic protein (GFAP) antisera has been used to detect astrocytes in subcultures, little immunocytochemistry has been carried out on primary cultures from normal adult human brain (Rorke et al, 1975;Gilden et al, 1976;Barna et al, 1985;Asch et al, 1986;Cheng-Mayer et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Immunocytochemical Characterization of Primary Glial Cell Cultures From Normal Adult Human Brain

Newcombe¹,

Meeson

Cuzner

1988

Neuropathology Appl Neurobio

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Newcombe J., Meeson A. & Cuzner M. L. (1988) Neuropathology and Applied Neurobiology 14, 453–465 Immunocytochemical characterization of primary glial cell cultures from normal adult human brain Primary cultures were established from autopsy or biopsy samples of normal adult human brain and characterized by immunocytochemical techniques. Initially, macrophages were the predominant cell type adhering to the substratum, but as their number fell that of glial cells increased. Oligodendrocytes comprised 30% of the glial population in white matter cultures, and their perikarya and elongated processes were immunostained with antibodies directed against galactocerebroside and four myelin proteins. In white and grey matter cultures, process–bearing astrocytes and small numbers of polygonal astrocytes were stained with antibodies against glial fibrillary acidic protein and glutamine synthetase. Fibroblasts started to appear at 3 weeks and proliferated to form a monolayer beneath glial cells by 5 weeks. Glia began to die in the 6th week. These primary cell cultures of white or grey matter can be used to study the properties of glial cells from normal or pathological adult human brain.

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Human brain in tissue culture. IV. Morphological characteristics

Cited by 36 publications

References 12 publications

Cellular composition of human glial cultures from adult biopsy brain tissue

Cellular composition of human glial cultures from adult biopsy brain tissue

Heterogeneity of astroglia cultured from adult human temporal lobe

Immunocytochemical Characterization of Primary Glial Cell Cultures From Normal Adult Human Brain

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