Human C-reactive protein: expression, structure, and function

Volanakis, John E.

doi:10.1016/s0161-5890(01)00042-6

Cited by 809 publications

(661 citation statements)

References 92 publications

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“…The decrease in intimal hyperplasia with increased CRP though contrary to expectations may not be surprising in retrospect. While blood CRP is increased in response to inflammatory stimuli, CRP per se has anti-inflammatory properties (for review [4,14]). CRP binds and promotes the clearance of apoptotic cells, sustaining an anti-inflammatory response [36].…”

Section: Discussionmentioning

confidence: 99%

“…CRP levels are associated with future cardiovascular events in seemingly healthy subjects and with worse prognosis in acute coronary patients [1]. CRP promotes thrombosis [2,3] and complement activation [4], and might impact lipoprotein metabolism [5]. The confluence of clinical and basic evidence suggests that CRP should predict outcome after vascular intervention.…”

Section: Introductionmentioning

confidence: 99%

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Neointimal formation is reduced after arterial injury in human crp transgenic mice

et al. 2008

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Objectives/Methods-Elevated CRP levels predict increased incidence of cardiovascular events and poor outcomes following interventions. There is the suggestion that CRP is also a mediator of vascular injury. Transgenic mice carrying the human CRP gene (CRPtg) are predisposed to arterial thrombosis post-injury. We examined whether CRP similarly modulates the proliferative and hyperplastic phases of vascular repair in CRPtg when thrombosis is controlled with daily aspirin and heparin at the time of trans-femoral arterial wire-injury.Results-Complete thrombotic arterial occlusion at 28 days was comparable for wild-type and CRPtg mice (14 and 19%, respectively). Neointimal area at 28d was 2.5 fold lower in CRPtg (4190 ± 3134 μm 2 , n = 12) compared to wild-types (10,157 ± 8890 μm 2 , n = 11, p < 0.05). Likewise, neointimal/media area ratio was 1.10 ± 0.87 in wild-types and 0.45 ± 0.24 in CRPtg (p < 0.05). Seven days post-injury, cellular proliferation and apoptotic cell number in the intima were both less pronounced in CRPtg than wild-type. No differences were seen in leukocyte infiltration or endothelial coverage. CRPtg mice had significantly reduced p38 MAPK signaling pathway activation following injury.Conclusions-The pro-thrombotic phenotype of CRPtg mice was suppressed by aspirin/ heparin, revealing CRP's influence on neointimal growth after trans-femoral arterial wire-injury. Signaling pathway activation, cellular proliferation, and neointimal formation were all reduced in CRPtg following vascular injury. Increasingly we are aware of CRP multipotent effects. Once considered only a risk factor, and recently a harmful agent, CRP is a far more complex regulator of vascular biology.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Neointimal formation is reduced after arterial injury in human crp transgenic mice

et al. 2008

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“…C-reactive protein (CRP), which is a component of the acute phase response in humans, participates in innate immunity against pneumococcal infection (Agrawal et al, 2008;Kushner, 1982;Simons et al, 2014;Volanakis, 2001). In humans, the concentration of CRP in circulation increases rapidly within hours of an inflammatory stimulus due to its increased hepatic synthesis under transcriptional and post-transcriptional control (Bode et al, 2012;Kim et al, 2015;Singh et al, 2007;Voleti and Agrawal, 2005;Voleti et al, 2012;Zhang et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Purification of recombinant C-reactive protein mutants

Thirumalai

Singh

Hammond

et al. 2017

Journal of Immunological Methods

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“…C-reactive protein (CRP), the classical marker of acute phase response in humans and most animals, is an indicator of a variety of pathologic processes including infections, tissue damage, and chronic inflammatory diseases [1]. Serum CRP concentrations may rise within 24-48 h after the acute event and may increase up to a 1000-fold.…”

Section: Introductionmentioning

confidence: 99%

A new high-sensitive nephelometric method for assaying serum C-reactive protein based on phosphocholine interaction

Drieghe

Alsaadi

Tugirimana

et al. 2014

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background: The measurement of C-reactive protein (CRP) concentrations has been of interest as a classical marker of acute phase response; in addition, it has been of particular interest in cardiovascular risk stratification where high-sensitive measurements are necessary. Since CRP is able to bind phospholipids (mainly phosphocholine) in the presence of calcium ions, we explored the possibilities of developing a high-sensitive affordable nephelometric CRP assay based on diluted soy oil emulsions. Methods: Serum (or heparinized plasma) was mixed with Intralipid 20% in Tris-calcium buffer (pH 7.5). After 12 min of incubation at 37°C, the CRP-phospholipid complexes were measured by nephelometry (840 nm) using a BN II nephelometer (Siemens). Results (n = 97) were compared with those obtained using a typical immunoturbidimetric method (Roche). Results: Imprecision of the functional nephelometric assay was evaluated using three human serum pools. Within-run coefficients of variation (CVs) for level 1, 2 and 3 were 6.1%, 4.7% and 4.5%, respectively, and between-run CVs were 17.6%, 18.8% and 11.3%, respectively. Good agreement was obtained between the functional nephelometric and the immunoturbidimetric CRP assay in a concentration range from 0.1 mg/L to 50 mg/L (r = 0.884). A logit-log calibration curve was made between 0.056 mg/L and 1.785 mg/L. The limit of detection was 0.5 mg/L. Conclusions: The functional nephelometric CRP assay allowed high-sensitive CRP determinations in serum and plasma. Since the assay is species independent, the described functional CRP assay could be used for veterinary purposes as well.

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Human C-reactive protein: expression, structure, and function

Cited by 809 publications

References 92 publications

Neointimal formation is reduced after arterial injury in human crp transgenic mice

Neointimal formation is reduced after arterial injury in human crp transgenic mice

Purification of recombinant C-reactive protein mutants

A new high-sensitive nephelometric method for assaying serum C-reactive protein based on phosphocholine interaction

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