Human calgizzarin; one colorectal cancer-related gene selected by a large scale random cDNA sequencing and Northern blot analysis

Tanaka, Masaki; Adzuma, K.; Iwami, Miki; Yoshimoto, Katsuhiko; Monden, Yasumasa; Itakura, Mitsuo

doi:10.1016/0304-3835(94)03687-e

Cited by 52 publications

(35 citation statements)

References 9 publications

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“…These genes play a role in the differentiation, signal transduction, cell cycle and cytoskeletal membrane functions (Donato, 2001;Mischke, 1998) by regulating calcium levels during squamous differentiation. The elevation of calgizzarin in colon carcinoma parallels our findings and indicates a role in squamous tumorigenesis (Tanaka et al, 1995). Another gene that was elevated in our study was the small nuclear RNA gene SM D1 which is important in the modification of ribosomal RNA and plays an important function in HNSC cell development Weinstein and Steitz, 1999).…”

Section: Discussionsupporting

confidence: 87%

Differential expression profiling of head and neck squamous carcinoma: significance in their phenotypic and biological classification

et al. 2002

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The genetic events associated with the development and progression of head and neck squamous carcinoma (HNSC) are largely unknown. We analysed 12 matched pairs of histologically normal squamous mucosa and tumor specimens from six conventional and six phenotypic variants HNSC to define the differentially expressed genes in these tumors. Parallel expression analysis of 8055 unique genes was performed, and the level of the hybridization signal for each gene was measured after normalization. Hierarchical cluster analysis of the expressed genes showed distinct interand intra-tumoral patterns in and between conventional squamous carcinoma and squamous carcinoma variants. We also identified 26 (0.32%) differentially expressed genes that were consistently different between matched pairs of normal and tumor specimens; a selected set of the overexpressed genes was validated using real-time quantitative RT -PCR. The majority of the genes were associated with differentiation and proliferation. Our study defines a set of genes that could form the basis for the construction of limited HNSC targeted expression array and indepth studies and further highlights gene profile differences that may be useful in pathobiologic classification of HNSC.

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Section: Discussionsupporting

confidence: 87%

Differential expression profiling of head and neck squamous carcinoma: significance in their phenotypic and biological classification

et al. 2002

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“…The sequence (Fig. 4) matches the previously published sequence of a S100A11 cDNA clone isolated from human colon carcinoma cells (43), indicating that the S100A11 protein detected in skin and in cultured keratinocytes (54,55), and the colon carcinoma protein are identical. Our sequential proteolytic digestion results suggest that S100A11 is crosslinked in specific locations.…”

Section: Labeled "Minor" (Eg C9 C9-minor)supporting

confidence: 79%

“…S100A11 (S100C, calgizzarin) has been identified in gizzard, lung, heart (74,75), skin (54), and cultured keratinocytes (55). Our cDNA sequence shows that epidermal S100A11 is identical to S100A11 isolated from human colon carcinoma cells (43). As mentioned above, the S100 proteins are thought to affect Ca 2ϩ signaling pathways by binding to and regulating the activity of target proteins.…”

Section: Discussionmentioning

confidence: 58%

“…Polymerase Chain Reaction Cloning of Calgizzarin (S100A11)-Poly(A) ϩ RNA, isolated from cultured human foreskin keratinocytes (53), was used as the template to clone a cDNA encoding the human S100A11 protein coding region by reverse transcription-polymerase chain reaction (RT-PCR) using 5Ј-CAT ATG GCA AAA ATC TCC AGC CC as the forward primer and 5Ј-GGA TCC TGA GGT GGT TAG TGT GCT CA (43) as the reverse primer. Poly(A) ϩ RNA (1 g) was reversetranscribed in a standard reaction using a RT-PCR kit (Boehringer Mannheim, catalog no.…”

Section: Methodsmentioning

confidence: 99%

“…P13, with the amino acid sequence GPAPCPAPAP, did not find a perfect match in the non-redundant protein data base. The closest matches were bovine ␤-crystallin B1 ( 34 GPAPAPA-PAP 43 ) and myosin light chain 1 (-PAPAPAPAP-) from several species. Since the HPLC profile obtained from fraction 8 ( Fig.…”

Section: Labeled "Minor" (Eg C9 C9-minor)mentioning

confidence: 99%

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S100A11, S100A10, Annexin I, Desmosomal Proteins, Small Proline-rich Proteins, Plasminogen Activator Inhibitor-2, and Involucrin Are Components of the Cornified Envelope of Cultured Human Epidermal Keratinocytes

Robinson

Lapic

Welter

et al. 1997

Journal of Biological Chemistry

205

197

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The cornified envelope (CE) is an insoluble sheath of ⑀-(␥-glutamyl)lysine cross-linked protein, which is deposited beneath the plasma membrane during keratinocyte terminal differentiation. We have probed the structure of the CE by proteolytic cleavage of purified CE fragments isolated from CEs formed spontaneously in cell culture. CNBr digestion, followed by trypsin and then proteinase K treatment released 25%, 42%, and 18%, respectively, of the CE protein. Purification and sequencing of released peptides has identified two novel CE precursors, S100A11 (S100C, calgizzarin) and S100A10 (calpactin light chain). We also sequenced peptides derived from annexin I and plasminogen activator inhibitor 2, two putative envelope precursors, as well as portions of the well established CE precursor proteins SPR1A, SPR1B, and involucrin. Many desmosomal components were identified (desmoglein 3, desmocolin A/B, desmoplakin I, plakoglobin, and plakophilin), indicating that desmosomes become cross-linked into the CE. Fragments derived from envoplakin, the recently sequenced 210-kDa membranous CE precursor protein, which also appears to be a desmosomal component, were also identified. Analysis of the pattern of peptide release following the sequential digestion indicates that S100A11 is anchored to the envelope via Gln 102 and/or Lys 103 at the carboxyl terminus and at Lys 3 , Lys 23 , and/or Gln 22 in the amino terminus. A similar type of analysis indicates that small proline-rich proteins 1A and 1B (SPR1A and SPR1B) become cross-linked at the amino terminus (residues 1-23) and the carboxyl terminus (residues 86 -89). No loricrin, cystatin A, or elafin peptides were detected.The cornified cell envelope (CE) 1 is a 15-nm-thick crosslinked sheath of protein that forms beneath the plasma membrane during the final stages of epidermal keratinocyte differentiation (1-3). Transglutaminase enzymes catalyze the assembly of this structure via formation of ⑀-(␥-glutamyl)lysine bonds between envelope precursors (4 -7). The proteins that have been identified as constituents of the CE include loricrin (8 -10), involucrin (11-14), the small proline-rich (SPR) family of proteins (15-18), cystatin A (19, 20), elafin (21-23), filaggrin (24 -26), keratin (27, 28), desmosomal components (27), annexin I (29 -31), plasminogen activator inhibitor-2 (PAI-2) (32), and the 195-kDa and 210-kDa proteins (33, 34). Cross-links have been identified within loricrin, elafin, filaggrin, keratin, SPR1, SPR2, and desmoplakin (35). Loricrin is present as a partner in most of these cross-links. It has been proposed that envelope formation is initiated with formation of an envelope scaffolding that consists of soluble precursors, and that other precursors (both soluble and insoluble) are later deposited (3,11,35). The scaffolding has been proposed to consist of involucrin, cystatin A, and possibly other proteins (25). In this model, other proteins, including loricrin, elafin, and SPRs, are deposited onto this scaffolding.In the present report, we study the compositi...

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Developmental stage- and germ cell-regulated expression of a calcium-binding protein mRNA in mouse Sertoli cells

et al. 1999

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Human calgizzarin; one colorectal cancer-related gene selected by a large scale random cDNA sequencing and Northern blot analysis

Cited by 52 publications

References 9 publications

Differential expression profiling of head and neck squamous carcinoma: significance in their phenotypic and biological classification

Differential expression profiling of head and neck squamous carcinoma: significance in their phenotypic and biological classification

S100A11, S100A10, Annexin I, Desmosomal Proteins, Small Proline-rich Proteins, Plasminogen Activator Inhibitor-2, and Involucrin Are Components of the Cornified Envelope of Cultured Human Epidermal Keratinocytes

Developmental stage- and germ cell-regulated expression of a calcium-binding protein mRNA in mouse Sertoli cells

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