Human cardiac tissue induces transdifferentiation of adult stem cells towards cardiomyocytes

Perán, Macarena; Marchal, Juan A.; López, Elena; Jiménez-Navarro, Manuel F.; Boulaiz, Houría; Rodríguez-Serrano, Fernando; Carrillo, Esmeralda; Sánchez-Espín, Gemma; Teresa, Eduardo de; Tosh, David; Aránega, Antonia

doi:10.3109/14653240903548202

Cited by 46 publications

(25 citation statements)

References 18 publications

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“…The inhibition in cell proliferation induced by 1,25-D3 is accompanied by an increase in cell differentiation evidenced by an increase in cardiomyotubes cell area and the expression of ctroponin, a main cardiac differentiation marker (Perán et al 2010).…”

Section: Discussionmentioning

confidence: 99%

1,25-vitamin D3 promotes cardiac differentiation through modulation of the WNT signaling pathway

Hlaing

Garcia

Contreras

et al. 2015

EJEA

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Cardiovascular disease (CV) remains the leading cause of death worldwide. Low levels of vitamin D are associated with high risk of myocardial infarction, even after controlling for factors associated with coronary artery disease. A growing body of evidence suggests that vitamin D plays an important role in CV related signaling pathways. However, little is known about the molecular mechanism by which vitamin D modulates cardiac development. The Wnt signaling pathway plays a pivotal role in tissue development by controlling stem cell renewal, lineage selection and even more importantly heart development. In this study, we examined the role of 1,25-D 3 (active form of vitamin D) on cardiomyocyte proliferation, apoptosis, cell phenotype, cell cycle progression and differentiation into cardiomyotubes. We determined that the addition of 1,25-D 3 to cardiomyocytes cells: 1) Inhibits cell proliferation without promoting apoptosis; 2) Decreases expression of genes related to the regulation of the cell cycle; 3) Promotes cardiomyotubes formation; 4) Induces the expression of Casein kinase-1-α1, a negative regulator of the canonical Wnt signaling pathway; and 5) Increases the expression of the noncanonical Wnt11, which it has been demonstrated to induce cardiac differentiation during embryonic development in adult cells. In conclusion, we postulate that vitamin D promotes cardiac differentiation through a negative modulation of the canonical Wnt signaling pathway and by upregulating the expression of Wnt11. These results suggest that vitamin D repletion to prevent and/or improve cardiovascular disorders that are linked to abnormal cardiac differentiation, such as post infarction cardiac remodeling, deserve further study.

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Section: Discussionmentioning

confidence: 99%

1,25-vitamin D3 promotes cardiac differentiation through modulation of the WNT signaling pathway

Hlaing

Garcia

Contreras

et al. 2015

EJEA

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“…Interestingly, TBX5 [71,72] and MEF2C [73] early cardiac markers and definitive cardiac markers such as ACTN1, cTNT, and GJAI were expressed [74]. TBX5 is a member of T-box gene family [75] critical for the development of the heart.…”

Section: Discussionmentioning

confidence: 99%

Development of Bioactive Patch for Maintenance of Implanted Cells at the Myocardial Infarcted Site

Castells-Sala

Vallés-Lluch

Soler‐Botija

et al. 2015

Journal of Nanomaterials

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Ischemia produced as a result of myocardial infarction might cause moderate or severe tissue death. Studies under development propose grafting stem cells into the affected area and we hypothesize that this mechanism could be enhanced by the application of a "bioactive implant. " The implant herein proposed consists of a thin porous elastomeric membrane, filled with self-assembling nanofibers and human subcutaneous adipose tissue derived progenitor cells. We describe the development and characterization of two elastomeric membranes: poly(ethyl acrylate) (PEA) and poly(caprolactone 2-(methacryloyloxy)ethyl ester) (PCLMA). Both are a good material support to deliver cells within a soft self-assembling peptide and are elastic enough to withstand the stresses arising from the heartbeat. Both developed composites (PEA and PCLMA, combined with self-assembling peptide) equally facilitate the propagation of electrical pulses and maintain their genetic profile of the seeded cells. Preliminary studies with small animal models suggest that, at short times, the bioimplant shows good adhesion with the myocardium. After three days cells loaded in the patch remain alive at the implanted site. We propose that the bioactive patch (elastomeric membranes with self-assembling peptide and cells) could increase the efficacy of future cardiac cell therapy by improving cell immobilization and survival at the affected site.

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“…After 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate striated cells and branching fibbers), immunofluorescence detection of cardiacspecific markers (connexin-43, sarcomeric -actinin, cardiac troponin I and T and desmin) and the presence of cardiomyocyte-related genes (cardiac myosin light chain 1, -cardiac actin, cardiac troponin T and cardiac -myosin). The relevance of these findings lies in the potential use of autologous extracts to induce stem cell reprogramming (Perán et al, 2010).…”

Section: Transdifferentiation Methods Using Adult Stem Cells 311 Usmentioning

confidence: 99%

Therapeutic Approaches in Regenerative Medicine of Cardiovascular Diseases: From Bench to Bedside

Aránega¹,

Bustamante²,

Marchal³

et al. 2011

Advances in Regenerative Medicine

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Human cardiac tissue induces transdifferentiation of adult stem cells towards cardiomyocytes

Cited by 46 publications

References 18 publications

1,25-vitamin D3 promotes cardiac differentiation through modulation of the WNT signaling pathway

1,25-vitamin D3 promotes cardiac differentiation through modulation of the WNT signaling pathway

Development of Bioactive Patch for Maintenance of Implanted Cells at the Myocardial Infarcted Site

Therapeutic Approaches in Regenerative Medicine of Cardiovascular Diseases: From Bench to Bedside

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