Human cartilage-bone-synovium microphysiological system to study ptoa pathogenesis and treatment on earth and in space

Abstract

apparent integrity of the cartilage structure. Showed the most evident changes in the extremes of the condyle. However, at 20 DAI, the changes were significant, showed clearly clusters in the superficial and middle zone of the cartilage, and the evident loss of PGs contained. In addition, the expression of collagen I (Col-I), and MMP-3 and MMP-13 were increased, mainly around to the clusters. In contrast, although S samples showed a little increased in the expression of Col I, and reduction of PGs contained at 5 DAI. That changes were not significant in comparing with normal samples. Conclusions:The new OA mouse model results less invasive, due to the mice can walk normally, and its lesion permits follows a kinetic from the early to the late stages of OA. In addition, all the changes identified, are congruent with others OA models, such as some presents in rat, and even can to be scored using the OARSI histopathology recommendations.

Modeling early changes associated with cartilage trauma using human-cell-laden hydrogel cartilage models

“…Mechanical loads have been to date difficult to apply to synovial joint MPS, including traumatic loads, the causative event in the development of PTOA and responsible A review of studies on chondrocyte construct loading reveals the use of dynamic cyclic compression with physiological loading frequencies with rest periods replicating daily activity and continued exercise over multiple days. In the existing synovial joint MPS [29][30][31][32][33][34][35][36], the mechanical stimulation used has a strain of 0-35% at a frequency of 0.2-1 Hz [51]. These conditions are good for establishing joint tissue homeostasis and possibly modeling chronic overload as a cause of OA under the right (to be determined) conditions.…”

Tissue catabolism and donor-specific dexamethasone response in a human osteochondral model of post-traumatic osteoarthritis

“…However, novel methods of drug delivery are currently under investigation to replicate low-dose Dex delivery over a longer durations targeted to cartilage [55][56][57]. The addition of synovial tissue to this system would broaden applicability to knee and other joints, but would introduce uncontrolled and inconsistent levels of inflammatory cytokines compared to controlled addition of exogenous cytokines [58]. Our proteomic analysis used MS identification based on trypsin digestion of media and tissue proteins, which removes the ability to identify some endogenously produced peptides.…”