Human CC10, the homologue of rabbit uteroglobin: genomic cloning, chromosomal localization and expression in endometrial cell lines

Wolf, Markus; Klug, Jörg; Hackenberg, R.; Gessler, Manfred; Grzeschik, Karl‐Heinz; Beato, Miguel; Suske, Guntram

doi:10.1093/hmg/1.6.371

Cited by 69 publications

(51 citation statements)

References 23 publications

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“…The use of the 5' region of human ccI OkD gene has been suggested in gene transfer experiments (4). This might elucidate the significance of cis-acting elements for the cell-specific expression of the human gene and might lead to the possible use of portions of its 5' region in gene therapy for obtaining organ-specific expression of a wild-type gene in the respiratory epithelia.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Tissue-specific expression of the gene coding for human Clara cell 10-kD protein, a phospholipase A2-inhibitory protein.

Peri

Cordella‐Miele²,

Miele³

et al. 1993

J. Clin. Invest.

119

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Section: Discussionmentioning

confidence: 99%

“…The name ccI OkD derives from the apparent molecular mass ofthis protein in nonreducing SDS-PAGE (1). The cDNA (3) as well as the gene (4) for this protein have been cloned and characterized. The exact physiological role of cc10kD is yet to be clarified.…”

Section: Introductionmentioning

confidence: 99%

Tissue-specific expression of the gene coding for human Clara cell 10-kD protein, a phospholipase A2-inhibitory protein.

Peri

Cordella‐Miele²,

Miele³

et al. 1993

J. Clin. Invest.

119

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“…Region I encompasses the noncanonical TATA-box region (TACA), which is recognized by the tissuespecific factor TCF and the ubiquitous factor TPF (27), which was identified in this study as YY1. (B) Comparison of the rabbit uteroglobin (UG) TACA box (56), the cognate human (63), mouse (54), and rat (18) TATA sequences, the TATA box of the Ad2 major late promoter (6), and the TACA box of the Ad2 EIIaL promoter (21) with the TATA consensus sequence, using the weight matrix description of Bucher (6). A negative weight matrix score indicates the extent of deviation from the consensus sequence (score ϭ 0).…”

Section: Methodsmentioning

confidence: 99%

Binding of YY1 to a Site Overlapping a Weak TATA Box Is Essential for Transcription from the Uteroglobin Promoter in Endometrial Cells

Klug

Beato

1996

Molecular and Cellular Biology

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The gene for rabbit uteroglobin codes for a small calcium-, steroid-, and biphenyl metabolite-binding homodimeric protein which is expressed in a variety of epithelial cell types such as Clara cells (lung) and the glandular and luminal cells of the endometrium. One important region mediating its efficient transcription in a human endometrium-derived cell line, Ishikawa, is centered around a noncanonical TATA box. Two factors, TATA core factor (TCF), expressed in cell lines derived from uteroglobin-expressing tissues, and the ubiquitously expressed TATA palindrome factor, bind to the DNA major groove at two adjacent sites within this region. Here, we report the identification of the TATA palindrome factor as the transcription/initiation factor YY1 by microsequencing of the biochemically purified factor from HeLa cells. The binding site for YY1 within the uteroglobin gene is unique in its sequence and its location overlapping a weak TATA box (TACA). Binding of YY1 was required for efficient transcription in TCF-positive Ishikawa cells, which responded only weakly to a change of TACA to TATA, although in vitro binding affinity for the TATA-box-binding protein increased by 1 order of magnitude. In contrast, in CV-1 cells, lacking TCF, binding of YY1 was not required for transcription in the context of a wild-type TACA box, whereas a change from TACA to TATA led to significantly increased reporter gene expression. DNA binding data exclude a role of YY1 in stabilizing the interaction of the TATA-box-binding protein with the uteroglobin promoter. We conclude that cell lines derived from uteroglobin-expressing tissues overcome the weak TATA box with the help of auxiliary factors, one of them being YY1.

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“…1]). Alignment of the rabbit uteroglobin TATA-box sequence with the corresponding sequences of the human and rat genes (whose gene products were formerly called Clara cell 10-kDa secretory proteins [CC10] [60]) shows that the human and rat genes possess a canonical TATA box, whereas the rabbit promoter has a TACA box with a cytosine instead of a thymine in the third position of the consensus sequence. Though TBP/TFIID has been reported to bind to noncanonical TATA box sequences (47,59), all available data on TACA boxes point to the fact that it is a weak recognition site for TBP (7,24,39).…”

mentioning

confidence: 99%

Two distinct factors bind to the rabbit uteroglobin TATA-box region and are required for efficient transcription.

Klug¹,

Knapp²,

Castro³

et al. 1994

Mol. Cell. Biol.

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The rabbit uteroglobin gene is expressed in a variety of epithelial cell types like the lung Clara cells and the glandular and luminal epithelial cells of the endometrium. Expression in Clara cells is on a high constitutive level, whereas expression in the rabbit endometrium is under tight hormonal control. One important element of the rabbit uteroglobin gene mediating its efficient transcription in two epithelial cell lines from human endometrium (Ishikawa) and lung (NCI-H441) is its noncanonical TATA box (TACA). Here, we show that two factors (TATA core factor [TCF] and TATA palindrome factor [TPF]) different from the TATA-box binding protein bind to the DNA major groove at two adjacent sites within the uteroglobin TATA-box region and that one of them (TCF) is specifically expressed in cell lines derived from uteroglobin-expressing tissues. The binding sites for TCF and TPF, respectively, are both required for efficient transcription in Ishikawa and NCI-H441 cells. Mutation of the TACA box, which we show is a poor TATA box in functional terms, to a canonical TATA motif does not affect TCF and TPF binding. Therefore, we suggest that the function of the unusual cytosine could be to reduce rabbit uteroglobin expression in cells lacking TCF and that the interaction of TATA-box binding protein with the weak TACA site is facilitated in TCF-and TPF-positive cells.

show abstract

Human CC10, the homologue of rabbit uteroglobin: genomic cloning, chromosomal localization and expression in endometrial cell lines

Cited by 69 publications

References 23 publications

Tissue-specific expression of the gene coding for human Clara cell 10-kD protein, a phospholipase A2-inhibitory protein.

Tissue-specific expression of the gene coding for human Clara cell 10-kD protein, a phospholipase A2-inhibitory protein.

Binding of YY1 to a Site Overlapping a Weak TATA Box Is Essential for Transcription from the Uteroglobin Promoter in Endometrial Cells

Two distinct factors bind to the rabbit uteroglobin TATA-box region and are required for efficient transcription.

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