2020
DOI: 10.1101/2020.09.07.285114
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Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers

Abstract: We used thermostable group II intron reverse transcriptase sequencing (TGIRT-seq), which gives end-to-end sequence reads of highly structured RNAs, to identify >8,000 short (≤300 nt) full-length excised intron (FLEXI) RNAs in human cells. Most FLEXI RNAs are predicted to have stable secondary structures, making them difficult to detect by other RNA-seq methods. Some FLEXI RNAs correspond to annotated mirtron pre-miRNAs (introns that are processed into functional miRNAs) or agotrons (introns that bind AGO2 a… Show more

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Cited by 2 publications
(1 citation statement)
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“…The circular RNAs described here are rare (Fig 1) and in most cases likely harmless, but an active ILS might also catalyze events we have yet to detect in vivo . Unusual full length linear forms of introns have been described (Morgan et al 2019; Yao et al 2022), and in yeast have been implicated in growth control (Parenteau et al 2019; Morgan et al 2019), but whether these are formed within the ILS is not yet clear. In addition, circle formation does not conform to the established tail length rule for the much more abundant linear introns described previously (Morgan et al 2019).…”
Section: Discussionmentioning
confidence: 99%
“…The circular RNAs described here are rare (Fig 1) and in most cases likely harmless, but an active ILS might also catalyze events we have yet to detect in vivo . Unusual full length linear forms of introns have been described (Morgan et al 2019; Yao et al 2022), and in yeast have been implicated in growth control (Parenteau et al 2019; Morgan et al 2019), but whether these are formed within the ILS is not yet clear. In addition, circle formation does not conform to the established tail length rule for the much more abundant linear introns described previously (Morgan et al 2019).…”
Section: Discussionmentioning
confidence: 99%