2022
DOI: 10.1038/s41598-022-13071-x
|View full text |Cite
|
Sign up to set email alerts
|

Human cerebral spheroids undergo 4-aminopyridine-induced, activity associated changes in cellular composition and microrna expression

Abstract: Activity-induced neurogenesis has been extensively studied in rodents but the lack of ante mortem accessibility to human brain at the cellular and molecular levels limits studies of the process in humans. Using cerebral spheroids derived from human induced pluripotent stem cells (iPSCs), we investigated the effects of 4-aminopyridine (4AP) on neuronal activity and associated neurogenesis. Our studies demonstrate that 4AP increases neuronal activity in 3-month-old cerebral spheroids while increasing numbers of … Show more

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

0
5
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
3
1
1

Relationship

1
4

Authors

Journals

citations
Cited by 5 publications
(5 citation statements)
references
References 58 publications
0
5
0
Order By: Relevance
“…As neuronal activity was lower in the presence of microglia, we sought to investigate whether neurons were still functional and able to respond to stimulation or whether this capacity was disrupted in presence of microglia. Increased neuronal activity in response to the potassium channel blocker 4-Aminopyridine (4-AP) has previously been characterised in other cell models (Parmentier et al, 2022). Neuronal activity in both cocultures and tricultures from three independent biological repeats was modulated with 4-AP (Fig 5).…”
Section: Resultsmentioning
confidence: 99%
“…As neuronal activity was lower in the presence of microglia, we sought to investigate whether neurons were still functional and able to respond to stimulation or whether this capacity was disrupted in presence of microglia. Increased neuronal activity in response to the potassium channel blocker 4-Aminopyridine (4-AP) has previously been characterised in other cell models (Parmentier et al, 2022). Neuronal activity in both cocultures and tricultures from three independent biological repeats was modulated with 4-AP (Fig 5).…”
Section: Resultsmentioning
confidence: 99%
“…As neuronal activity was lower in the presence of microglia, we sought to investigate whether neurons were still functional and able to respond to stimulation or whether this capacity was disrupted in presence of microglia. Increased neuronal activity in response to the potassium channel blocker 4-AP has previously been characterised in other cell models ( Parmentier et al, 2022 ). Neuronal activity in both cocultures and tricultures from three independent biological repeats was modulated with 4-AP ( Figure 5 ).…”
Section: Resultsmentioning
confidence: 77%
“…Mature neurons are commonly observed after 120 days in culture. Astrocytes appear between 60 and 90 days in culture, and their proportion increases steadily with time in culture (Parmentier et al., 2022; Paşca et al., 2015; Trujillo et al., 2019). A postnatal gene expression signature was noted in cerebral organoids around 250 days (Gordon et al., 2021).…”
Section: Commentarymentioning
confidence: 99%
“…By recapitulating the early, in utero stages of central system development, human cerebral organoids allow researchers to evaluate the neurotoxicity of a compound on a previously inaccessible window of human neurodevelopment. In a recent study, we used this approach to describe the effect of 4‐AP, a potassium channel blocker, on early neurogenesis in human cerebral organoids (Parmentier et al., 2022). Since the first description of cerebral organoids in mouse and humans, several protocols have been published both from embryonic stem cells and iPSCs (Birey et al., 2017; Eiraku et al., 2008; Giandomenico et al., 2019; Kadoshima et al., 2013; Lancaster et al., 2013; Paşca et al., 2015; Velasco et al., 2019; Yoon et al., 2019; Zafeiriou, Guobin, et al., 2020).…”
Section: Commentarymentioning
confidence: 99%
See 1 more Smart Citation