Human Chorionic Gonadotrophin and Alpha‐fetoprotein Levels in Matched Samples of Amniotic Fluid, Extraembryonic Coelomic Fluid, and Maternal Serum in the First Trimester of Pregnancy

Wathen, N. C.; Cass, P. L.; Kitau, M. J.; Chard, Tim

doi:10.1002/pd.1970110303

Cited by 45 publications

(35 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Coelomic fluid can be obtained by ultrasound-guided coelo centesis and several studies have documented its biochemical composition [1,2], In a pre liminary report we demonstrated that tradi tional cytogenetic analysis of coelomic cells is unsuccessful [3]. However, cells within coe lomic fluid have been successfully examined by fluorescence in situ hybridisation (FISH).…”

Section: Introductionmentioning

confidence: 99%

Rapid Determination of Fetal Sex in Coelomic and Amniotic Fluid by Fluorescence in situ Hybridisation

Pandya

Cardy²,

Jauniaux³

et al. 1995

Fetal Diagn Ther

View full text Add to dashboard Cite

Coelomic fluid (n = 32), amniotic fluid (n = 26) and placental tissue were obtained from 32 women undergoing termination of pregnancy at 7–11 weeks of gestation. Fluorescence in situ hybridisation (FISH) was performed to determine fetal sex using a heterochromatic Y probe and an alpha satellite repeat probe for chromosome X. In each case there was concordance in the fetal sex from the three compartments. However, no result could be obtained from 4 of the 32 coelomic fluids and 4 of the 26 amniotic fluid samples. The hybridisation efficiency was significantly lower in coelomic and amniotic fluid compared with placental tissue.

show abstract

Section: Introductionmentioning

confidence: 99%

Rapid Determination of Fetal Sex in Coelomic and Amniotic Fluid by Fluorescence in situ Hybridisation

Pandya

Cardy²,

Jauniaux³

et al. 1995

Fetal Diagn Ther

View full text Add to dashboard Cite

show abstract

“…Matched samples (n ϭ 7) of extra-embryonic coelomic and amniotic fluids were collected by ultrasound guided needle aspiration from women between 8-11 weeks gestation, prior to the termination of pregnancy by general anaesthesia, as described in detail previously (Wathen et al, 1991). Fetal viability and gestational age were confirmed by ultrasound.…”

Section: Collection Of Samplesmentioning

confidence: 99%

Secretion of matrix metalloproteinase-2, matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases into the intrauterine compartments during early pregnancy

Riley

Leask²,

Chard³

et al. 1999

Molecular Human Reproduction

View full text Add to dashboard Cite

Matrix metalloproteinases (MMPs) are important enzymes in tissue remodelling, a key event for the development of the fetal membranes and placenta and establishing the feto-maternal interface during early pregnancy. This study has examined the secretion of the gelatinases, MMP-2 (72 kDa) and MMP-9 (92 kDa), and the endogenous tissue inhibitors of metalloproteinases (TIMPs) into extra-embryonic coelomic and amniotic fluids, the two principal intra-uterine compartments of the first trimester, and compared them to amniotic fluid collected later in gestation. In extra-embryonic coelomic fluid, gelatin zymography demonstrated that MMP-2 (72 kDa) was the predominant gelatinase, with some MMP-9 present. A broad range of TIMPs corresponding to TIMP-1 and TIMP-2, glycosylated and unglycosylated TIMP-3 and TIMP-4 was detected in this compartment by reverse zymography and immunoblot analyses. There was little gelatinase or TIMP activity in amniotic fluid in the first trimester. In amniotic fluid from the second trimester after fusion of the membranes obliterating the extra-embryonic coelom, and at term elective caesarean section, MMP-2 is the predominant gelatinase present, with a broad spectrum of TIMPs. These findings demonstrate that predominantly MMP-2 and also MMP-9, regulated by a range of TIMPs, are involved in intra-uterine tissue remodelling during the establishment of pregnancy.

show abstract

“…ical composition of the celomic fluid [14][15][16][17][18][19][20][21][22][23][24] , (2) diagnosis of a variety of genetic disorders [11,[25][26][27][28][29][30] , and (3) in sheep, for in-utero somatic stem cell therapy [6] . However, as mentioned earlier, development of the extra-embryonic celome and functions of the celomic fluid and placenta in sheep and primates are different.…”

Section: Discussionmentioning

confidence: 99%

A Study to Determine if Human Umbilical Cord Hematopoietic Stem Cells Can Survive in Baboon Extra-Embryonic Celomic Fluid: A Prerequisite for Determining the Feasibility of in-utero Stem Cell Xeno-Transplantation via Celocentesis

et al. 2006

View full text Add to dashboard Cite

Objectives: To determine if: (1) human umbilical cord stem cells could survive for 20 h in extra-embryonic celomic fluid obtained at 40 days of development from baboon pregnancies by ultrasound-guided celocentesis and, (2) human hematopoietic stem cell survival could be enhanced by adding increasing concentrations of hematopoietic stem cell culture medium to the celomic fluid. Methods: CD34+ cells were isolated from umbilical cord blood using a magnetic activated cell sorter and flow cytometry. Cells were then cultured in five platforms containing different combinations of baboon extra-embryonic celomic fluid and hematopoietic stem cell culture medium to determine cell survival kinetics over a 20-hour period in each of the conditions. Results: Human umbilical cord stem cells can survive for at least 20 h in baboon’s celomic fluid. Chi-square for linear trends demonstrated that the number of viable cells was significantly enhanced by adding the increasing concentrations of culture medium to the celomic fluid (29% cell survival in pure celomic fluid, 48% at 1:8, 50% at 1:2, 54.5% at 1:1, 61% in pure culture medium; p < 0.001). Conclusions: Human umbilical cord stem cells survive in baboon celomic fluid and cell survival improves when the celomic fluid is mixed with greater concentrations of hematopoietic stem cell culture medium. Based on these findings, future in-vivo experiments in the pregnant baboon animal model can be directed at determining whether in-utero injection of human hematopoietic stem cells prior to 10 weeks of pregnancy can lead to permanent chimeras.

show abstract

Human Chorionic Gonadotrophin and Alpha‐fetoprotein Levels in Matched Samples of Amniotic Fluid, Extraembryonic Coelomic Fluid, and Maternal Serum in the First Trimester of Pregnancy

Cited by 45 publications

References 10 publications

Rapid Determination of Fetal Sex in Coelomic and Amniotic Fluid by Fluorescence in situ Hybridisation

Rapid Determination of Fetal Sex in Coelomic and Amniotic Fluid by Fluorescence in situ Hybridisation

Secretion of matrix metalloproteinase-2, matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases into the intrauterine compartments during early pregnancy

A Study to Determine if Human Umbilical Cord Hematopoietic Stem Cells Can Survive in Baboon Extra-Embryonic Celomic Fluid: A Prerequisite for Determining the Feasibility of in-utero Stem Cell Xeno-Transplantation via Celocentesis

Contact Info

Product

Resources

About