Human chorionic gonadotrophin pharmaceutical formulations of urinary origin display high levels of contaminant proteins–A label‐free quantitation proteomics study

Abstract

To determine whether there is a measurable protein background in different formulations of urinary and recombinant human chorionic gonadotropin (hCG). Primary outcome measures: identification of contaminant proteins in urinary‐derived formulations of hCG; secondary outcome measures: quantitative values of contaminant proteins in different batches of urinary –derived hCG formulations. It was found that urinary‐derived batches have high presence of contaminant proteins beside the active substance. The relative amount of contaminant proteins and hCG differs strongly between different batches.

Proteomic Analysis of Porcine-Derived Collagen Membrane and Matrix

“…The membranes and matrices were sonicated for 15 min and centrifuged for 5 min at 4 °C and 10,000 rpm. The extracted proteins were digested as previously described [ 27 ]. Briefly, the protein concentration was determined using the nano spectrophotometer (DS-11 FX, DeNovix, Wilmington, NC, USA), and the proteins were reduced using 5 mM Dithiothreitol (DTT, Sigma-Aldrich, St. Louis, MO, USA) for 30 min at 60 °C and alkylated for 30 min using 15 mM Iodoacetamide (IAA, Sigma-Aldrich, St. Louis, MO, USA) in the dark.…”

“…Highly sensitive mass spectrometers and improved separation methods have enabled analysis of minute sample amounts of proteins from a wider area of biological materials, such as plasma, serum, urine, bone, and saliva, just to name a few [ 25 , 26 , 27 , 28 , 29 ]. Furthermore, the use of proteomics enables the nature of interactions between biomaterials and biological systems to be determined, e.g., for tissue-biomaterials or cell-biomaterials [ 30 ].…”

Mass Spectrometry in Clinical Laboratories

“…Tandem MS capacities enhance selectivity and signal-to-noise ratio and provide essential structural information based on which it is possible to identify the structural conformation of analyzed samples. For these reasons, many laboratories use triple quadrupole (Q ) MS/MS over ion trap (IT) MS instruments in routine practice for detection and analysis of antibiotics and other drug residues [51][52][53][54][55]. This advantage is reflected in its quantitative features regarding IT MS with its MS n capabilities, which are highly beneficial for analysis of analyte's molecular structure and identification.…”