Human chromostatin inhibits endothelin-1-induced contractures in human blood vessels

Aardal, S.; Galindo, Estelle; Aunis, Dominique; Helle, K.B.

doi:10.1016/0167-0115(93)90269-e

Cited by 15 publications

(11 citation statements)

References 24 publications

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“…We have been interested by CGA, because it was demonstrated that this prohormone was able to exert not only a feedback regulation on catecholamine release but also a direct effect on human blood vessels by inhibiting vasoconstriction produced by endothelin-1 (10). Moreover, the N-terminal peptide (1-40) stimulates the secretion of calcitonin gene-related peptide, the vasodilatative functions of which are well known.…”

Section: Discussionmentioning

confidence: 99%

“…Pancreastatin, a 49-residue peptide, has been described in porcine pancreas as a potent inhibitor of insulin secretion from endocrine pancreatic islets (6). CGA-derived peptides have been found to exert a feedback control on chromaffin cell secretory activity (7)(8)(9), and chromostatin, a 20-residue peptide, inhibits endothelin-1-induced vasoconstriction in human blood vessels (10).…”

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confidence: 99%

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Circulating Chromogranin A and Catecholamines in Human Fetuses at Uneventful Birth

et al. 1995

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Chromogranin A (CGA), a large acidic 48-kD protein, costored and coreleased by exocytosis with catecholamines, has been shown to be a precursor of peptides that exert feedback regulatory control on catecholamine secretion. In plasma, CGA levels increase in response to a large-amplitude physical stimulation in adult subjects and may be related to catecholamine levels. Any akin information is not yet available when the sympathoadrenal system is highly actived during birth. This activation is strongly related to parturition circumstances such as the mode of delivery. The aim of our study was to determine CGA plasma levels in infants delivered vaginally or by elective cesarean section and to investigate the possible correlation between CGA and catecholamine concentrations. Plasma levels of catecholamines (norepinephrine and epinephrine) and CGA were assessed by HPLC with electrochemical detection and immunoenzymology, respectively. CGA and norepinephrine concentrations were significantly higher ( p < 0.0002 and p < 0.02) in infants vaginally born than in the group delivered by elective cesarean section. A significant relationship (p < 0.04) was found between CGA and norepinephrine levels. However, for epinephrine, no significant difference was found between both groups. These results demonstrate the fetus' ability to corelease CGA and norepinephrine massively in response to stress of birth. (Pediatr Res 37: [101][102][103][104][105] 1995)

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Circulating Chromogranin A and Catecholamines in Human Fetuses at Uneventful Birth

et al. 1995

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“…The second group containing bands c, d and g at 45, 37 and 27 kDa, respectively, corresponds to shorter N-terminal CGA-derived fragments. The third group is composed of weakly labeled bands (e, f, h, i, j and k labels) which correspond to 32,30,24,23,18 and 17 kDa apparent molecular mass, respectively. Interestingly, the 24-kDa and 23-kDa bands (h and i, respectively) probably correspond to vasostatin II [rat CGA(1-128)] previously reported to migrate with an apparent molecular mass of 21-23 kDa [34].…”

Section: Vasostatin-containing Peptides In Rat Heartmentioning

confidence: 99%

“…affect microbial growth and nociception [5, [20][21][22][23]. Vasostatin-I [human CGA(1-76)] and its N-terminal domain [CGA(1-40)] have also been reported to affect the dilatation of vessels [24][25][26][27][28] probably via an endothelium-independent vasoinhibitory action [26]. In addition, circulating CGA concentrations are increased in cardiac pathology [29].…”

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confidence: 99%

Characterization of natural vasostatin‐containing peptides in rat heart

Glattard

Angelone

Strub³

et al. 2006

The FEBS Journal

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Chromogranin A (CGA) is a protein that is stored and released together with neurotransmitters and hormones in the nervous, endocrine and diffuse neuroendocrine systems. As human vasostatins I and II [CGA(1–76) and CGA(1–113), respectively] have been reported to affect vessel motility and exert concentration‐dependent cardiosuppressive effects on isolated whole heart preparations of eel, frog and rat (i.e. negative inotropism and antiadrenergic activity), we investigated the presence of vasostatin‐containing peptides in rat heart. Rat heart extracts were purified by RP‐HPLC, and the resulting fractions analyzed for the presence of CGA N‐terminal fragments using dot‐blot analysis. CGA‐immunoreactive fractions were submitted to western blot and MS analysis using the TOF/TOF technique. Four endogenous N‐terminal CGA‐derived peptides [CGA(4–113), CGA(1–124), CGA(1–135) and CGA(1–199)] containing the vasostatin sequence were characterized. The following post‐translational modifications of these fragments were identified: phosphorylation at Ser96, O‐glycosylation (trisaccharide, NAcGal‐Gal‐NeuAc) at Thr126, and oxidation at three methionine residues. This first identification of CGA‐derived peptides containing the vasostatin motif in rat heart supports their role in cardiac physiology by an autocrine/paracrine mechanism.

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“…2 They act as helper proteins in the packaging of peptides, hormones, or neuropeptides, 3,4 and they also modulate the processing of the secretory granule components. 1 Extracellularly, they are postulated to serve as prohormones to generate biologically active peptides, which may influence hormonal release 5 and have vascular 6 and chemoattractant functions. 7 Recently, Strub and colleagues 8 demonstrated that several peptides displaying antimicrobial properties (at micromolar range) were naturally generated from CGB and CGA in bovine chromaffin granules.…”

Section: Introductionmentioning

confidence: 99%

Presence of chromogranin-derived antimicrobial peptides in plasma during coronary artery bypass surgery and evidence of an immune origin of these peptides

Tasiemski

Hammad

Vandenbulcke

et al. 2002

Blood

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Chromogranin A (CGA) and chromogranin B (CGB) are acidic proteins stored in secretory organelles of endocrine cells and neurons. In addition to their roles as helper proteins in the packaging of pep-tides, they may serve as prohormones to generate biologically active peptides such as vasostatin-1 and secretolytin. These molecules derived from CGA and CGB, respectively, possess antimicrobial properties. The present study demonstrates that plasmatic levels of both vasostatin-1 and secretolytin increase during surgery in patients undergoing cardiopulmonary bypass (CPB). Vasostatin-1 and secretoly-tin, initially present in plasma at low levels , are released just after skin incision. Consequently, they can be added to enk-elytin, an antibacterial peptide derived from proenkephalin A, for the panoply of components acting as a first protective barrier against hypothetical invasion of pathogens, which may occur during surgery. CGA and CGB, more commonly viewed as markers for endocrine and neuronal cells, were also found to have an immune origin. RNA messengers coding for CGB were amplified by reverse transcription-polymerase chain reaction in human monocytes, and immunocyto-chemical analysis by confocal micros-copy revealed the presence of CGA or CGB or both in monocytes and neutro-phils. A combination of techniques including confocal microscopic analysis, mass spectrometry measurement, and antibac-terial tests allowed for the identification of the positive role of interleukin 6 (IL-6) in the secretolytin release from mono-cytes in vitro. Because IL-6 release is known to be strongly enhanced during CPB, we suggest a possible relationship between IL-6 and the increased level of secretolytin in patients undergoing CPB. (Blood. 2002;100:553-559)

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Human chromostatin inhibits endothelin-1-induced contractures in human blood vessels

Cited by 15 publications

References 24 publications

Circulating Chromogranin A and Catecholamines in Human Fetuses at Uneventful Birth

Circulating Chromogranin A and Catecholamines in Human Fetuses at Uneventful Birth

Characterization of natural vasostatin‐containing peptides in rat heart

Presence of chromogranin-derived antimicrobial peptides in plasma during coronary artery bypass surgery and evidence of an immune origin of these peptides

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