Human Colon and Liver Cancer Cell Proliferation Inhibition by Peptide Hydrolysates Derived from Heat-Stabilized Defatted Rice Bran

Abstract

Rice bran, an economical, underutilized coproduct of rough rice milling, was used to produce peptide hydrolysates, which were investigated for anticancer activity. Protein hydrolysates prepared by Alcalase hydrolysis under optimized conditions were treated further to obtain gastrointestinal (GI)-resistant peptide hydrolysates. They were fractionated into >50, 10-50, 5-10, and <5 kDa sizes and evaluated for inhibitory activity on proliferation of human colon (Caco-2) and liver (HepG2) cancer cell lines by Trypan blue dye exclusion assay. GI-resistant <5 and 5-10 kDa sized peptide fractions inhibited growth of Caco-2 cells by 80%, and the <5 kDa fraction inhibited growth of HepG2 cells by approximately 50% compared to controls and nonresistant fractions. An MTS cell titer assay confirmed antiproliferative effects of the peptide fractions. The results demonstrated that 5-10 and <5 kDa sized GI-resistant fractions promoted significant (p < 0.05) inhibitory activities on both cancer cell lines compared to controls. More investigations are needed to show such value-added effects on the technofunctional and sensorial properties of the food protein and peptide matrices.

Peptides Derived From Rice Bran Protect Cells From Obesity and Alzheimer’s Disease

“…The hydrolysates were treated with simulated gastrointestinal resistant solutions to generate GIE resistant peptides, which were then fractionated into <5, 5-10, 10-50, and >50 kDa fractions as previously done 11 . 2.2.2 Anti-obesity effect: Evaluation of degree of differentiation of adipocytes for accumulation of lipids: Undifferentiated human preadipocytes (Lonza, USA) were allowed to differentiate upon treatment with a known differentiating agent [0.25µmol/L DEX (IS-IBMX-DEX) mixture] (Sigma USA).…”

Stepwise encapsulation and controlled two-stage release system for cis-Diamminediiodoplatinum

“…However, recurrence of liver cancer is very common. 25,26 We therefore believe that cis-DIDP-loaded SLNs can be used as an implantable drug delivery system to prevent the recurrence of liver cancer after surgery.…”

Soybean peptide fractions inhibit human blood, breast and prostate cancer cell proliferation

“…Advanced cancer treatment methods employ a holistic approach by incorporating dietary or nutritional, spiritual and physical therapies along with drugs and radiation. As part of nutritional supplementation, bioactive peptides have a prominent role in alternative medicine which involves controlling diseases using food derived macro molecules (Zhang et al 1998;Korhonen and Pihlanto 2003;Kannan et al 2008). Certain other bioactive molecules are from natural sources like plants (Ng et al 2003;Ohba et al 2004;Silva-Sánchez et al 2008).…”

“…Certain other bioactive molecules are from natural sources like plants (Ng et al 2003;Ohba et al 2004;Silva-Sánchez et al 2008). One of the major approaches to produce bioactive peptides was enzymatic hydrolysis or fermentation, especially from sources like soybean, wheat, corn, rice, sunflower, milk, eggs, and meat (Gibbs et al 2004;Wang and de Mejia 2005;de Mejia and de Lumen 2006;Kannan et al 2008Kannan et al , 2009Kannan et al , 2010Hettiarachchy 2013).…”