Human Colon Sialidase: Characterization and
Activity Levels in Normal Mucosa and Colonic
Adenocarcinoma

Vs, Martínez-Zorzano; Feijoo, C.; M, Páez de la Cadena; M, Butrón; Fernández-Briera, Almudena; Fj, Rodríguez-Berrocal

doi:10.1159/000475001

Cited by 4 publications

(3 citation statements)

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“…A low Neu1 level in undifferentiated cells, therefore possibly causing hypersialylation of the carbohydrate portion of glycoproteins, is in line with the observation that malignant cells are often hypersialylated (6,36). We previously observed decreased Neu1 activity in transformed rat 3Y1 fibroblasts (37) and suppression of malignant phenotype of B16 melanoma cells by Neu1 overexpression (22), although one controversial report (38) of increased sialidase activity in human colon tumor probably reflected change in the same Neu1 enzyme, because of the use of 4-methylumbellyferyl-neuraminic acid as substrate. Decreased Neu3 in differentiated cells, on the other hand, would lead to reduction in ganglioside degradation, consistent with previous reports presenting evidence for an increase of GM3 synthase during NaBT treatment (23).…”

Section: Discussionsupporting

confidence: 65%

Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression

Kakugawa

Wada

Yamaguchi

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

222

171

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Human plasma membrane-associated sialidase (Neu3) is unique in specifically hydrolyzing gangliosides, thought to participate in cell differentiation and transmembrane signaling, thereby playing crucial roles in the regulation of cell surface functions. We have discovered levels of mRNA for this sialidase to be increased in restricted cases of human colon cancer by 3-to 100-fold compared with adjacent nontumor mucosa (n ‫؍‬ 32), associated with significant elevation in sialidase activity in tumors (n ‫؍‬ 50). In situ hybridization showed the sialidase expression in epithelial elements of adenocarcinomas. In cultured human colon cancer cells, the sialidase level was downregulated in the process of differentiation and apoptosis induced by sodium butyrate, whereas lysosomal sialidase (Neu1) was upregulated. Transfection of the sialidase gene into colon cancer cells inhibited apoptosis and was accompanied by increased Bcl-2 and decreased caspase expression. Colon cancer exhibited a marked accumulation of lactosylceramide, a possible sialidase product, and addition of the glycolipid to the culture reduced apoptotic cells during sodium butyrate treatment. These results indicate that high expression of the sialidase in cancer cells leads to protection against programmed cell death, probably modulation of gangliosides. This finding provides a possible sialidase target for diagnosis and therapy of colon cancer.

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Section: Discussionsupporting

confidence: 65%

Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression

Kakugawa

Wada

Yamaguchi

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

222

171

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“…Experimental data obtained in our laboratory showed an increased sialidase activity in colorectal carcinoma compared with healthy tissue [24]. This elevated sialidase activity could also be involved in the alterations in the expresion of ·(2,3)-and ·(2,6)-linked sialic acid residues that we observed in tumors from colorectal cancer patients because the ·(2,6)-linkage seems to be much more resistant to mammalian sialidases [25].…”

Section: Discussionsupporting

confidence: 55%

Immunohistochemical Analysis of Sialic Acid and Fucose Composition in Human Colorectal Adenocarcinoma

Fernández-Rodrı́guez¹,

Feijoo-Carnero

Merino-Trigo

et al. 2000

Tumor Biol

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The expression of different sialoglycoconjugates and fucoglycoconjugates in normal mucosa and adenocarcinoma samples from 43 colorectal cancer patients was investigated by using specific lectins and applying a semiquantitative analysis. A pronounced decrease in the intracellular binding of the Maackia amurensis lectin, which recognizes α(2,3)-linked sialic acid residues, was found in the tumoral tissue. In contrast, a significant increase in the staining with the Sambucus nigra lectin (SNA I), which binds to α(2,6)-linked sialic acid residues, was detected in the epithelial cells as well as in the mucins from tumors. No significant differences in the reactivity with the Aleuria aurantia lectin, which recognizes the sequence Fuc(α1,6)GlcNAc, between normal and malignant colorectal tissues were detected. Furthermore, the correlation between lectin-binding profiles and the prognosis of colorectal cancer patients was examined. After an average postoperative follow-up period of 31 months, patients with tumors showing a strong SNA I staining presented a greater probability of disease recurrence. This result suggests that the intensity of staining with SNA I could be a valid parameter for predicting recurrence in colorectal cancer.

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“…There are also several reports on the alterations of endogenous sialidase activity in cancers. Martinez-Zorzano et al (11) have reported that sialidase activity toward synthetic substrate 4-methylumbelliferyl-N-acetylneuraminic acid was increased in human colon cancer as compared to normal mucosa. Moreover, increased sialidase activity was observed in breast cancer (12).…”

Section: Introductionmentioning

confidence: 99%

Sialylation of intracellular proteins of thyroid lesions

Krześlak

Gaj²,

Pomorski³

et al. 2007

Oncol Rep

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Enhanced sialylation represents one of the most frequently occurring alterations of the sugar chain structure in various cancers. However, up to now, sialylation of intracellular proteins of thyroid carcinomas has never been investigated. The aim of this study was comparative analysis of cytoplasmic and nuclear sialoglycoproteins isolated from thyroid benign and malignant tumors as well as non-neoplastic lesions. The sialylation level and types of sialic acid linkages were analysed by lectin blotting and enzyme linked lectino-solidphase assay (ELLSA) using Sambucus nigra (SNA) and Maakia amurensis (MAA) agglutinins. The presence of •2,6 and •2,3 linked sialic acid residues was detected in all types of thyroid lesion specimens but there were some differences in the banding and intensity patterns. Analysis of SNA and MAA binding by ELLSA method showed that in the majority of cancer samples the level of sialic acid residues was lower than in non-neoplastic lesions and adenomas. Our present results suggest that decrease in sialylation rather than increase is a characteristic feature of malignant transformation in the thyroid.

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Human Colon Sialidase: Characterization and Activity Levels in Normal Mucosa and Colonic Adenocarcinoma

Cited by 4 publications

References 0 publications

Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression

Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression

Immunohistochemical Analysis of Sialic Acid and Fucose Composition in Human Colorectal Adenocarcinoma

Sialylation of intracellular proteins of thyroid lesions

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