Human colonic microbiota associated with diet, obesity and weight loss

Duncan, Sylvia H.; Lobley, G. E.; Holtrop, Grietje; Ince, Jennifer; Johnstone, Alexandra M.; Louis, Petra; Flint, Harry J.

doi:10.1038/ijo.2008.155

Cited by 1,081 publications

(822 citation statements)

References 21 publications

Supporting

Mentioning

718

Contrasting

Unclassified

Order By: Relevance

“…Mice in the HF group were characterized by increased relative abundance of Rikenellaceae, which is in agreement with other reports based on qPCR and FISH that found no decrease in Bacteroidetes following HF feeding (Cani et al, 2008;Duncan et al, 2008). The presence of Alistipes, a genus within the Rikenellaceae, has also been recently associated with type-2 diabetes in humans (Qin et al, 2012).…”

Section: Discussionsupporting

confidence: 91%

High-fat diet alters gut microbiota physiology in mice

Daniel¹,

Gholami²,

Berry³

et al. 2013

The ISME Journal

588

386

View full text Add to dashboard Cite

The intestinal microbiota is known to regulate host energy homeostasis and can be influenced by highcalorie diets. However, changes affecting the ecosystem at the functional level are still not well characterized. We measured shifts in cecal bacterial communities in mice fed a carbohydrate or high-fat (HF) diet for 12 weeks at the level of the following: (i) diversity and taxa distribution by high-throughput 16S ribosomal RNA gene sequencing; (ii) bulk and single-cell chemical composition by Fourier-transform infrared-(FT-IR) and Raman micro-spectroscopy and (iii) metaproteome and metabolome via highresolution mass spectrometry. High-fat diet caused shifts in the diversity of dominant gut bacteria and altered the proportion of Ruminococcaceae (decrease) and Rikenellaceae (increase). FT-IR spectroscopy revealed that the impact of the diet on cecal chemical fingerprints is greater than the impact of microbiota composition. Diet-driven changes in biochemical fingerprints of members of the Bacteroidales and Lachnospiraceae were also observed at the level of single cells, indicating that there were distinct differences in cellular composition of dominant phylotypes under different diets. Metaproteome and metabolome analyses based on the occurrence of 1760 bacterial proteins and 86 annotated metabolites revealed distinct HF diet-specific profiles. Alteration of hormonal and anti-microbial networks, bile acid and bilirubin metabolism and shifts towards amino acid and simple sugars metabolism were observed. We conclude that a HF diet markedly affects the gut bacterial ecosystem at the functional level.

show abstract

Section: Discussionsupporting

confidence: 91%

High-fat diet alters gut microbiota physiology in mice

Daniel¹,

Gholami²,

Berry³

et al. 2013

The ISME Journal

588

386

View full text Add to dashboard Cite

show abstract

“…In accordance with these data, a high Firmicutes/Bacteroidetes ratio in the gut microbiome has been reported in many other systemic inflammatory conditions [52,57]. Other investigations, however, have either reported a significant decrease in this ratio [45,46,58] or no changes at all [59] in systemic inflammatory conditions. Although the ratio Firmicutes/Bacteroidetes was high in our diseased population, these phyla varied widely in abundance among patients within the same clinical group (Figure S1).…”

Section: Discussionsupporting

confidence: 77%

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study

Lourenςo

Spencer

Alm

et al. 2018

Journal of Oral Microbiology

122

109

View full text Add to dashboard Cite

Background: This exploratory study aimed to characterize the gut microbiome of individuals with different periodontal conditions, and correlate it with periodontal inflammation and tissue destruction.Methods: Stool samples were obtained from individuals presenting periodontal health (PH = 7), gingivitis (G = 14) and chronic periodontitis (CP = 23). The intestinal microbiome composition was determined by Illumina MiSeq sequencing.Results: A lower alpha-diversity in the gut microbiome of individuals with CP was observed, although no significant difference among groups was found (p > 0.01). Firmicutes, Proteobacteria, Verrucomicrobia and Euryarchaeota were increased, whereas Bacteroidetes were decreased in abundance in patients with periodontitis compared to PH. Prevotella (genus), Comamonadaceae (family) and Lactobacillales (order) were detected in higher numbers in G, while Bacteroidales (order) was predominant in PH (p < 0.01). Significant correlations (rho = 0.337–0.468, p < 0.01) were found between OTUs representative of periodontal pathogens and attachment loss. Mogibacteriaceae, Ruminococcaceae and Prevotella were able to discriminate individuals with periodontal diseases from PH (overall accuracy = 84%). Oral taxa were detected in high numbers in all stool samples.Conclusions: Individuals with periodontal diseases present a less diverse gut microbiome consistent with other systemic inflammatory diseases. High numbers of oral taxa related to periodontal destruction and inflammation were detected in the gut microbiome of individuals regardless of periodontal status.

show abstract

“…in the alimentary limb and the common channel of the body-weight matched rats (data not shown). Although changes in gut microbiota seen after RYGB resembled those obtained after weight loss in obese rodents [5], our data indicate that RYGB may have specific effects on intestinal microbiota.…”

Section: Resultsmentioning

confidence: 57%

“…Obesity was associated with higher Firmicutes and lower Bifidobacterium spp, Bacteroides-related bacteria and Lactobacillus spp in comparison with the lean counterparts [1][2][3][4]. Interestingly, weight loss achieved by dieting was able to reverse those changes [5]. Furthermore, nutrients with prebiotic properties induced qualitative changes in the composition of the gastrointestinal microbiota and peptide release (e.g., glucagon-like peptide-1 (GLP-1)) similar to those seen after dieting.…”

Section: Introductionmentioning

confidence: 98%

Roux-en-Y gastric bypass surgery in rats alters gut microbiota profile along the intestine

Osto

Abegg

Bueter

et al. 2013

Physiology & Behavior

View full text Add to dashboard Cite

Roux-en-Y gastric bypass (RYGB) surgery might modify the gut microbiota composition differently in the three distinct anatomical sections of the small intestine compared to sham surgery. We showed that RYGB induced changes in the microbiota of the alimentary limb and the common channel resembling those seen after prebiotic treatment or weight loss by dieting. These changes may be associated with altered production of intestinal hormones known to control energy balance. Postsurgical modulation of gut microbiota may significantly contribute to the beneficial metabolic effects of RYGB surgery. Abstract: Roux-en-Y gastric bypass (RYGB) surgery might modify the gut microbiota composition differently in the three distinct anatomical sections of small intestine compared to sham surgery. We showed that RYGB induced changes in the microbiota of the alimentary limb and the common channel resembling those seen after prebiotics treatment or weight loss by dieting. These changes may be associated with altered production of intestinal hormones known to control energy balance. Postsurgical modulation of gut microbiota may significantly contribute to the beneficial metabolic effects of RYGB surgery. 1 Title pageTitle: Roux-en-y gastric bypass surgery in rats alters gut microbiota profile along the intestine. Running head:Bariatric surgery alters gut microbiota. Authors:MELANIA AbstractRoux-en-Y gastric bypass (RYGB) surgery might modify the gut microbiota composition differently in the three distinct anatomical sections of small intestine compared to sham surgery. We showed that RYGB induced changes in the microbiota of the alimentary limb and the common channel resembling those seen after prebiotics treatment or weight loss by dieting. These changes may be associated with altered production of intestinal hormones known to control energy balance. Postsurgical modulation of gut microbiota may significantly contribute to the beneficial metabolic effects of RYGB surgery.

show abstract

Human colonic microbiota associated with diet, obesity and weight loss

Cited by 1,081 publications

References 21 publications

High-fat diet alters gut microbiota physiology in mice

High-fat diet alters gut microbiota physiology in mice

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study

Roux-en-Y gastric bypass surgery in rats alters gut microbiota profile along the intestine

Contact Info

Product

Resources

About