Human cord blood long-term engrafting cells are CD34+ CD38−

Abstract

There have been controversies about CD34 and CD38 expression by human cord blood (CB) stem cells. Using the newborn NOD/SCID/b2-microglobulin-null mouse assay that we recently developed, we examined the in vivo engrafting capability of human CB cells. Almost all of the 4-5 months engrafting cells were found in CD34 + population. The capability of secondary reconstitution was found only in the CD34 + cells. When the CD34 + CB cells were separated into CD38 À and CD38 + subpopulations and tested for engraftment, the majority of the engrafting cells were detected in the CD38 À subpopulation. These findings are consistent with the results from studies of murine stem cells and strongly indicate that the phenotype of human CB stem cells is CD34 + CD38 À . Leukemia (2003) 17, 960-964.

Factors Contributing to HIV-1 Induced Pathogenesis in the Human Thymus

“…More than 80% of these humanized mice, either referred to as "human adaptive immune system Rag2 -/- c -/-mice" (huAIS-RG) (Traggiai et al, 2004) or "human immune system BALB-Rag2 -/- c -/-" (HIS BALB-Rag/) (Gimeno et al, 2004), exhibited at least 10% or more human CD45 + cells in peripheral blood and other lymphoid organs. Similar improvements were observed in newborn NOD/SCID/ c -/-(NOG) mice (Ishikawa et al, 2003;Ishikawa et al, 2005;Ito et al, 2002;Shultz et al, 2005) and to a lesser extent in newborn NOD/SCID/2m -/-mice when used as recipient of HSC (Kollet et al, 2000). Part of the human CD4 + T cells in the spleen and peripheral blood of NOG mice express the co-receptors CXCR4 and CCR5 (Watanabe et al, 2007).…”

Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples

“…Pos as the part enriched for cells capable of providing short-term and intermediate stage hematopoiesis in humans (24). It is worth noting that our data not only demonstrated the highest percentage of CD34 Pos CD45…”

Hematopoietic developmental pathways: on cellular basis

“…À (Terstappen et al, 1991;Ishikawa et al, 2003) Sca-1 c-Kit þ GMLPs might be the precursor for ELPs and CLPs. pTa þ CLP-2 might be derived from the CLP.…”