Human Corneal Donor Material

Hoefle, Frank B.; Maurice, David M.; Sibley, Riley C.

doi:10.1001/archopht.1970.00990040743008

Cited by 40 publications

(4 citation statements)

References 2 publications

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“…AND PENETRATING GRAFTS Hoefle et al (1970) were the first to draw attention to the usefulness of the specular microscope for the examination of the endothelium of intact human donor eyes. Bigar et al (1976) did a large scale study on 278 donor eyes.…”

Section: Evaluation Of the Corneal Endothelium Of Donor Eyesmentioning

confidence: 99%

“…The specular microscope according to Maurice (1968) was used by Hoefle et al (1970) for the examination of intact human donor eyes prior to keratoplasty, but mostly for in vitro experiments for the study of morphology as well as for the registration of corneal thickness changes to determine the deturgescens capacity of the endothelium by measuring the temperature reversal effect (Dikstein & Maurice, 1972;Sherrard, 1973). For this purpose a dial gauge was added to the instrument to measure corneal thickness.…”

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confidence: 99%

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The clinical specular microscope

Binkhorst¹,

Loones²,

Nygaard³

1977

Doc Ophthalmol

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Section: Evaluation Of the Corneal Endothelium Of Donor Eyesmentioning

confidence: 99%

mentioning

confidence: 99%

The clinical specular microscope

Binkhorst¹,

Loones²,

Nygaard³

1977

Doc Ophthalmol

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“…Spiegelmikroskop von Maurice (1) wurde schon frUh zur Untersuchung des Endothels beim intakten Spenderauge angegeben (2,3,4 The specular microscopic examination of the endothehum of intact donor eyes can be rendered difficult or even impossible due to light scattering of stromal edema. We have been able to visualize large areas of the corneal endothelium of a donor eye with advanced stromal edemastored for over 32 h. in a moist chamberwith a confocal slit-scanning video microscope.…”

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confidence: 99%

Untersuchung der Hornhaut intakter Spenderaugen mit konfokalem Spalt-Scanning Video-Mikroskop

Bigar

Thaer²

1994

Klin Monatsbl Augenheilkd

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The specular microscopic examination of the endothelium of intact donor eyes can be rendered difficult or even impossible due to light scattering of stromal edema. We have been able to visualize large areas of the corneal endothelium of a donor eye with advanced stromal edema--stored for over 32 h. in a moist chamber--with a confocal slit-scanning video microscope. Furthermore, this optical arrangement enables the examination of very thin tissue sections with high resolution and a remarkably enhanced contrast of the corneal micromorphology (epithelial cells, Bowman's membrane, nerve fibers, keratocytes, endothelial cells).

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“…(3) Direct visual examination of the corneal endothelium through the rest of the cornea of intact donor eyes with high power specular-reflection microscopy (Hoefle, Maurice, and Sibley, 1970) is the most simple and least traumatic method, but it does not necessarily follow that an endothelium which appears 'normal' morphologically is functionally competent, and the converse is true. This paper reports the results of animal experiments on another method of evaluating donor corneal endothelium, but now from the point of view of its net functional efficiency in maintaining stromal deturgescence, a major requirement of most corneal grafts.…”

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Method of evaluating donor corneae for transplantation.

Sherrard¹

1973

British Journal of Ophthalmology

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A favourable prognosis for a penetrating corneal graft is largely dependent upon the integrity of the endothelium of the donor cornea, so that any method which will allow the condition of this delicate cell layer to be evaluated in potential transplant material will assist in promoting success.At present three such methods are in limited use: (i) The histochemical method of Robbins, Capella, and Kaufman (I965) and Kaufman, Capella, and Robbins (I966) employs staining with para nitro blue tetrazolium to demonstrate the viability of corneal cells. The procedure kills all cells, and it is therefore necessary to assume that the condition of the corneal endothelium is the same in both eyes of a donor pair at any given time. The endothelium of one cornea is evaluated, and if satisfactory, the graft material is taken from the other.(2) The permeability test (Stocker, King, Lucas, and Georgiade, 1966, I970) uses the permeability of the endothelial cells to the vital dye trypan blue as an index of cell viability. The method evaluates the endothelium of the cornea actually to be used as the graft, but subjects it to much risk of damage during the manipulation involved.(3) Direct visual examination of the corneal endothelium through the rest of the cornea of intact donor eyes with high power specular-reflection microscopy (Hoefle, Maurice, and Sibley, 1970) is the most simple and least traumatic method, but it does not necessarily follow that an endothelium which appears 'normal' morphologically is functionally competent, and the converse is true. This paper reports the results of animal experiments on another method of evaluating donor corneal endothelium, but now from the point of view of its net functional efficiency in maintaining stromal deturgescence, a major requirement of most corneal grafts. The endothelium of the actual transplant material is evaluated, in the intact eye, with no risk of damage to the cells. Concurrently the thickness of swollen corneae is reduced closer to normal, and the morphology of the endothelium can be examined by the technique of Hoefle and others (I970).The method depends upon the temperature reversal phenomenon of the cornea (Davson, I 955;Harris and Nordquist, I955). When a fresh normal eye is refrigerated to below io'C. in a moist environment, as in an orthodox eye bank, its cornea swells, and when rewarmed to physiological temperature the cornea thins down again to normal values. This reversal in corneal thickness in relation to temperature is due to great retardation of the corneal metabolic rate in the cold, resulting in the cessation of the outward going active fluid transport system which is located in the endothelium (Mishima and Kudo, I967; Maurice,

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Human Corneal Donor Material

Cited by 40 publications

References 2 publications

The clinical specular microscope

The clinical specular microscope

Untersuchung der Hornhaut intakter Spenderaugen mit konfokalem Spalt-Scanning Video-Mikroskop

Method of evaluating donor corneae for transplantation.

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