Human Corneal Donor Material

Hoefle, Frank B.

doi:10.1001/archopht.1969.00990020363013

Cited by 18 publications

(4 citation statements)

References 8 publications

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“…The incision was completed with curved corneal scissors and the ciliary body attachments to the scleral spur were separated. Care was taken to maintain the AC.7 Because the eyes of the very young donors (1,2,3 in Table I) were too small to fit into currently available commercial eye stands, they were wrapped in gauze and hand held during corneal excision. Some difficulty was encountered in these cases during separation of the ciliary body from the scleral spur.…”

Section: Methodsmentioning

confidence: 99%

Manipulative damage to the endothelium of infant and adult donor corneas.

Nartey

Sherrard

Steele

1990

British Journal of Ophthalmology

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Six human donor corneas were studied with the scanning electron microscope to quantify the hazards to the endothelium during the excision of corneoscleral buttons. Although the number studied was small, it was found that: (1) striae were more numerous in the flaccid, very young, donor corneas (under 1 year old) than in the more rigid adult corneas;(2) iridocorneal endothelial touch can result in loss of the posterior membrane and death of the touched endothelial cells. This corroborates the findings of other investigators. Both findings are important, because these conditions reduce the quality of the donor cornea for transplantation.

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Section: Methodsmentioning

confidence: 99%

Manipulative damage to the endothelium of infant and adult donor corneas.

Nartey

Sherrard

Steele

1990

British Journal of Ophthalmology

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“…Previous work has demonstrated that human corneal endothelial permeability to sucrose and urea increased as a function of both time and age of the donor during storage at 4°C: in a moist chamber (Hoefle 1969). It has been demonstrated that the endothelial permeability of older donors increased at a more rapid rate than that of younger donors, and that the permeability of a 4-month-old donor was unchanged after 80 h of storage (Hoefle 1969). T h e unchanged permeability in healthy rabbit corneas used in this investigation may be compared to the unchanged permeability in the 4-month-old human cornea, It is currently not known if human corneas stored in MK medium undergo a change in endothelial permeability as do older eyes stored in moist chambers.…”

Section: Commentsmentioning

confidence: 99%

Section: Commentsmentioning

confidence: 99%

Corneal Endothelial Permeability Following Storage in Moist Chamber and Mk Medium

Hull

Green

Bowman

1979

Acta Ophthalmologica

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“…1964;Hoeffle, 1969;Sherrard, 1973;McCarcy andKaufman, 1974: McCarey, 1976;Friedland and Forster, 1976;Bin der, 1980] and excised cornea in McCareyKaufman (M-K) medium [McCarey and Kaufman, 1974;McCarey, 1976;McCarey et al, , 1976Stark et al, 1975;Van Horn et al, 1975;Breslin et al, 1976;Friedland and Forster, 1976;Aquavella et al, 1976;Binder and Wickham, 1978;Binder, 1980;Sayegh, 1982;Harbour and Stern, 1983;Neubauer et al, 1984] at 4°C have suggested that the maximum storage time for each technique is 24-48 h and 96 h respec tively. Although clinical experience within these limits has been successful with each of these storage methods, there remains little and conflicting information on the meta bolic status of the cornea and on the dura tion of corneal viability.…”

Section: Introductionmentioning

confidence: 99%

Effects of Moist-Chamber and McCarey-Kaufman Medium Storage on the Metabolic Status of the Cornea: A 31P-Magnetic Resonance Analysis

Greiner

Sk³

et al. 1988

Ophthalmic Res

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The rate of change in concentration of corneal phosphatic metabolites of cat corneas stored in moist chamber and McCarey-Kaufman (M-K) medium was determined in order to provide a basis for prediction of the corneal metabolic status at a given storage time. Perchloric acid corneal extracts were examined by phosphorus-31 magnetic resonance after storage at 4 °C of whole globes under moist-chamber conditions up to 48 h and of excised corneas in M-K medium up to 168 h. A significant decline in the corneal concentrations of ATP and a significant increase in inorganic phosphate occurred for both storage methods; however, depending on the metabolite, the rate of decline or increase was significantly greater for the moist-chamber-stored corneas. The phosphorylated sugars significantly increased and the glycerophosphodiesters significantly decreased in the moist-chamber-stored corneas, whereas both metabolites remained unchanged in the M-K-medium-stored corneas. There was no significant change in the dinucleotides and nucleoside diphospho-sugars during the time course fot both storage methods. A threefold greater rate of decline was noted in the tissue energy modulus for the moist-chamber-stored corneas than for the M-K-medium-stored corneas (-0.0465 vs. -0.0121 modulus values/h). M-K medium was significantly more effective in the maintenance of high-energy phosphatic metabolites. The mathematical model for these rate determinations provides a basis for prediction of the corneal metabolic status at a given time in moist-chamber or M-K medium storage.

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Human Corneal Donor Material

Cited by 18 publications

References 8 publications

Manipulative damage to the endothelium of infant and adult donor corneas.

Manipulative damage to the endothelium of infant and adult donor corneas.

Corneal Endothelial Permeability Following Storage in Moist Chamber and Mk Medium

Effects of Moist-Chamber and McCarey-Kaufman Medium Storage on the Metabolic Status of the Cornea: A 31P-Magnetic Resonance Analysis

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