Human Corneal Epithelial Cell Shedding and Fluorescein Staining in Response to Silicone Hydrogel Lenses and Contact Lens Disinfecting Solutions

Gorbet, Maud; Peterson, Rachael C.; McCanna, David J.; Woods, Craig A.; Jones, Lyndon; Fonn, Desmond

doi:10.3109/02713683.2013.841255

Cited by 23 publications

(14 citation statements)

References 39 publications

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“…This suggested that the increased staining was unrelated to increased shedding due to impaired epithelial health. However, Gorbet et al (2014), using the same technique, reported increased epithelial cell shedding during acute exposure to MPS release from an SiHy lens.…”

Section: Discussionmentioning

confidence: 87%

Clinical staining of the ocular surface: Mechanisms and interpretations

Bron

Argüeso

İrkeç

et al. 2015

Progress in Retinal and Eye Research

140

109

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Section: Discussionmentioning

confidence: 87%

Clinical staining of the ocular surface: Mechanisms and interpretations

Bron

Argüeso

İrkeç

et al. 2015

Progress in Retinal and Eye Research

140

109

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“…Interestingly, the tear turnover rate has been published to fall with less concentrated fluorescein attributable to a contribution from persistent corneal fluorescence (King-Smith et al, 2013). In fluorophotometry experiments (de Kruijf et al, 1987; McNamara et al, 1997), fluorescence persists for 15–20 min despite tear washout (Gorbet et al, 2014; Joshi et al, 1996). The degree to which the fluorescence emanates from the epithelium as opposed to the stroma is not established.…”

Section: Resultsmentioning

confidence: 99%

“…However, some details are inconsistent with this hypothesis. Caspase positive cells do not admit fluorescein (Gorbet et al, 2014) and many hyperfluorescent cells do not show apoptosis (Mokhtarzadeh et al, 2011). Therefore, an active transport exchange of monocarboxylate species that includes fluorescein has been proposed in stressed cells (Yeh et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Although faint background fluores-cence is generally ignored at the slit lamp, virtually every corneal epithelial cell fluoresces shortly after application with fluorescein when viewed under a fluorescent microscope (Feenstra and Tseng, 1992; Gorbet et al, 2014; Mokhtarzadeh et al, 2011; Thinda et al, 2010; Wilson et al, 1995). Penetration of fluorescein at various levels of the cornea has been described (McNamara et al, 1997; Webber and Jones, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Fluorescence lifetime imaging microscopy reveals quenching of fluorescein within corneal epithelium

Glasgow

2016

Experimental Eye Research

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Topical application of fluorescein results in background fluorescence of normal corneal epithelial cells. The fluorescence appears relatively weak and is often ignored clinically. The concentrations of fluorescein applied clinically exceed the threshold for self quenching. The possibility that exuberant topical concentrations of fluorescein result in quenching of fluorescence in tears and normal corneal epithelium is explored. Fluorescence lifetime measurements are sensitive to quenching and are less vulnerable to inner filter effect than steady state measurements. The types of fluorescence lifetime quenching often report informative molecular interactions. Therefore, fluorescence lifetime confocal imaging was performed in solutions, tears and corneal epithelium removed by membrane cytology following applied fluorescein. Amplitude averaged fluorescence lifetimes (τamp) were measured with time resolved single photon counting using a pulsed diode laser for excitation of fluorescein. Lifetime decays were fit to multi-exponential models with least squares analysis. Stern-Volmer plots for both intensity (I) and (τamp) were determined. Stern-Volmer plots demonstrated both dynamic and static quenching components (R2 = 0.98 exponential fit, I0/I). Plots of τamp versus concentration of fluorescein revealed a linear relationship. Immediately after fluorescein application, quenching was evident in tears (τamp < 1 ns) versus tears sampled after 5 min (τamp = 3.7 ns). Corneal epithelium showed quenching (τamp ≥ 2 ns) from 1 to 16 min post fluorescein instillation. Clinical concentrations of fluorescein show self-quenching but rapidly dilute as tears turnover. Intracellular quenching occurs in normal corneal epithelium. Lifetime decay curves suggest complex mechanisms are involved. Quenching is a plausible explanation for the low fluorescence background observed clinically.

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“…[1][2][3][4][5][6][7][8] By studying the occurrence of solution-related staining, some authors focused the attention on different combinations of (1) type of lens and (2) maintenance solution and found clear evidence of combined clinical effects. 6,7,9,10 The advent of silicone hydrogel (SH) CLs raised even more the attention on ocular surface reactions because of the physical and chemical characteristics of the materials. 5-8,10-13 Papas et al 8 pointed out that lens care products developed for conventional hydrogel CLs may not be entirely compatible with SH CLs.…”

mentioning

confidence: 99%