Human cytomegalovirus (HCMV)-specific CD4+ T lymphocyte response in AIDS patients with no past or current HCMV disease following HAART

Tamarit, Alicia; Alberola, Juan; Mir, Amparo; Benet, Isabel; Mira, Josep Vicent; Muñoz, Carlos; Galindo, Marı́a José; Navarro, David

doi:10.1016/j.jcv.2003.07.001

Cited by 2 publications

(3 citation statements)

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“…The inability of most AIDS patients with extremely low pre-HAART nadir CD4 þ T cell counts to reconstitute the LPR to HCMV despite responding optimally to HAART has been described [Tamarit et al, 2004]. Taken together, these data point to an inverse relationship between the likelihood of reconstituting the LPR to HCMV in HIV-1 infected patients under HAART and the degree of immune deterioration, as inferred by the number of peripheral CD4 þ T cells, before initiation of HAART.…”

Section: Discussionmentioning

confidence: 94%

“…Qualitative detection of HCMV DNA by PCR in polymorphonuclear leukocytes (PMNLs) and urine was performed following a previously published nested PCR protocol which amplifies a HCMV gB sequence [Meyer-König et al, 1998;Tamarit et al, 2004]. The detection limit of this PCR was 20 DNA copies.…”

Section: Hiv Viral Load and Pcr Detection Of Hcmv Dnamentioning

confidence: 99%

“…To date, most studies addressing the effect of HAART on HCMV-specific immunity have been conducted in HIV patients in late stage disease either at high risk of developing HCMV or already presenting the latter. In these patients, control of HCMV replication, which rapidly follows response to HAART [Deayton et al, 1999], has been linked to recovery of lymphoproliferative response (LPR) to HCMV [Li et al, 1998;Gerna et al, 2001;Hsieh et al, 2001;Jacobson et al, 2001]; however, a number of studies have provided data showing that a lack of progression to HCMV disease is not always associated with the presence of robust LPRs to HCMV [Villacres et al, 2001;Weinberg et al, 2001;Berenguer et al, 2002;Tamarit et al, 2004]-thus seriously questioning the value of the LPRs to HCMV as a surrogate marker for protection against HCMV disease. Likewise, when evaluation of CD4 þ T cell immunity against HCMV in these patients has been assessed by flow cytometric detection of intracellular cytokines following short-term antigenic stimulation of PBMCs, an invariable association between the presence of HCMV disease and negative cytokine responses has not been proven [Jacobson et al, 2001].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of human cytomegalovirus specific T cell immunity in human immunodeficiency virus infected patients in different disease stages following HAART and in long‐term non‐progressors

Tamarit

Alberola

Mira

et al. 2004

Journal of Medical Virology

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T cell immunity to human cytomegalovirus (HCMV) was assessed in HAART-treated HIV-1 infected patients (9 asymptomatic, CDC group A; and 22 symptomatic, CDC group B), and in eight HIV-1 long term non-progressors. Patients were either prospectively or cross-sectionally examined for CD4(+) T cell counts, HIV RNA load, HCMV leukoDNAemia, HCMV DNA in urine, lymphoproliferative response (LPR) to HCMV and phytohemagglutinin (PHA), and cytokine secretion (IFN-gamma and IL-4) by HCMV-stimulated peripheral blood mononuclear cell (PBMC) cultures. No patient either progressed to clinical AIDS or developed HCMV active infection during the study period. Twenty-nine patients responded to HAART, though 12 patients failed to recover the LPR to HCMV over the study period (three from CDC group A and nine from CDC group B). In contrast to healthy control individuals, most patients displaying positive LPRs LPRs to HCMV had unstable responses. Sustained LPRs to HCMV were significantly associated with high pre-HAART nadir CD4(+) T cell counts. Long-term suppression of HIV viremia correlated with recovery of LPR to HCMV. Sequential PBMC cultures from most patients secreted IFN-gamma (but not IL-4) at normal levels upon HCMV stimulation, irrespective of the pre-HAART nadir CD4(+) T cell counts and CDC group to which patients belonged. Failure to reconstitute IFN-gamma response was associated with very low pre-HAART nadir CD4(+) T cell counts. Control of HCMV infection in the cohort was associated with either recovery or maintenance of IFN-gamma response rather than with reconstitution of LPR to HCMV. A LPR to HCMV was absent in three out of eight long term non-progressors; contrarily, all patients showed preserved IFN-gamma responses.

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Section: Discussionmentioning

confidence: 94%

Section: Hiv Viral Load and Pcr Detection Of Hcmv Dnamentioning

confidence: 99%