Human cytomegalovirus infection in human renal arteries in vitro

Reinhardt, Barbara; Vaida, Bianca; Voisard, Rainer; Keller, Lutz; Breul, Jürgen; Metzger, Harald; Herter, Tina; Baur, Regine; Lüske, Anke; Mertens, Thomas

doi:10.1016/s0166-0934(03)00035-1

Cited by 19 publications

(28 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings conflict with reports that connected HCMV infection with the ability to promote an environment conducive to cell proliferation [10] and the induction of cellular proliferation [11]. In a recently described human ex vivo organ culture model of HCMV-infection [12] reactive cell proliferation after cellfree productive HCMV-infection was delayed but not increased [13].…”

Section: Introductioncontrasting

confidence: 67%

See 1 more Smart Citation

HCMV infection triggers smooth muscle cell proliferation in a 3D human coronary in vitro model

Voisard¹,

Göttling²,

Baur³

et al. 2013

Virol Discov

Self Cite

View full text Add to dashboard Cite

Background: Although a role of the human cytomegalovirus (HCMV) in atherosclerosis and restenosis is probable, clinical studies are not conclusive. The present study investigates in a threedimensional (3D) human coronary transfilter co-culture model the effect of cell-free and cell-associated HCMV-infection. Methods: Human coronary endothelial cells (HCAEC) and HCMSMC were seeded on both sides of a polycarbonate filter membrane. HCMV-infection was carried out by HCMV-infected MO. As controls MO-attack without HCMV-infection, cell free HCMV-infection, and the transfilter co-culture model without MO-attack and without HCMV-infection (Mock) were used. Results: At day 1, day 4, day 7, and day 14 the effects of HCMV-infection on MO adhesion and chemotaxis and on reactive proliferation of HCMSMC was studied. Cell-associated HCMV infection of HCAEC and HCMSMC was less intense and postponed in comparison to cell-free HCMV infection. Endothelial adhesion of MO after cellbased HCMV infection was decreased in comparison to non-infected MO, no clear effect was found on chemotaxis. Both after cell-associated and cell-free HCMV infection proliferation of HCMSMC was significantly increased. Conclusions: In the 3D human coronary transfilter co-culture model both cell-free and cell-associated HCMV-infection significantly increased proliferation of HCMSMC. If we assume that HCMV is involved in the pathogenesis of atherosclereosis and restenosis, the effect of antiviral treatments should be studied in experimental and clinical studies.

show abstract

Section: Introductioncontrasting

confidence: 67%

“…Sinzger, University of Tübingen. Virus stocks were produced in human foreskin fibroblasts [12], infectivity was determined by plaque titration of stock virus [18]. Ten-fold dilutions of virus stocks were carried out in quadruplicate.…”

Section: Hcmv-infectionmentioning

confidence: 99%

HCMV infection triggers smooth muscle cell proliferation in a 3D human coronary in vitro model

Voisard¹,

Göttling²,

Baur³

et al. 2013

Virol Discov

Self Cite

View full text Add to dashboard Cite

show abstract

“…2A). Previous studies of HCMV infection in various tissue explants, including first-trimester floating and anchoring placental villi and arterial and cervical tissues, have revealed evidence for viral replication within 4 to 7 days (15,17,53). Together, these findings demonstrate that the decidual explants remain viable and retain their natural morphology over the time needed to support and monitor HCMV infection and spread.…”

Section: Resultsmentioning

confidence: 56%

Modeling of Human Cytomegalovirus Maternal-Fetal Transmission in a Novel Decidual Organ Culture

Weisblum

Panet

Zakay‐Rones

et al. 2011

J Virol

View full text Add to dashboard Cite

Human cytomegalovirus (HCMV) is the leading cause of congenital infection, associated with severe birth defects and intrauterine growth retardation. The mechanism of HCMV transmission via the maternal-fetal interface is largely unknown, and there are no animal models for HCMV. The initial stages of infection are believed to occur in the maternal decidua. Here we employed a novel decidual organ culture, using both clinically derived and laboratory-derived viral strains, for the ex vivo modeling of HCMV transmission in the maternal-fetal interface. Viral spread in the tissue was demonstrated by the progression of infected-cell foci, with a 1.3-to 2-log increase in HCMV DNA and RNA levels between days 2 and 9 postinfection, the expression of immediate-early and late proteins, the appearance of typical histopathological features of natural infection, and dose-dependent inhibition of infection by ganciclovir and acyclovir. HCMV infected a wide range of cells in the decidua, including invasive cytotrophoblasts, macrophages, and endothelial, decidual, and dendritic cells. Cell-to-cell viral spread was revealed by focal extension of infected-cell clusters, inability to recover infectious extracellular virus, and high relative proportions (88 to 93%) of cell-associated viral DNA. Intriguingly, neutralizing HCMV hyperimmune globulins exhibited inhibitory activity against viral spread in the decidua even when added at 24 h postinfection-providing a mechanistic basis for their clinical use in prenatal prevention. The ex vivo-infected decidual cultures offer unique insight into patterns of viral tropism and spread, defining initial stages of congenital HCMV transmission, and can facilitate evaluation of the effects of new antiviral interventions within the maternal-fetal interface milieu.

show abstract

“…Because it has previously been shown that infection of organ cultures with HCMV exhibits strain-specific differences, we used the highly endotheliotropic clinical strain VR1814 in our experiments (20,26). Mammary arteries, obtained from HCMV-seronegative patients during coronary artery bypass surgery (CABS), were dissected under the microscope into suitable segments, followed by 1-h incubation either in HCMV-containing cell culture supernatant (10 5 plaque forming units/mL) or in virus-free cell culture medium lacking virus.…”

Section: Resultsmentioning

confidence: 99%

“…Because virus-strain specific differences had been reported in previous infection studies using organ cultures (26,40), we used an HCMV clinical isolate with a well documented endotheliotropism for this study (20). Furthermore, all arteries used for infection were from HCMV-seronegative donors, excluding a potential reactivation of latent HCMV within the transplanted vessel.…”

Section: Discussionmentioning

confidence: 99%

Human Cytomegalovirus Infection Leads to Elevated Levels of Transplant Arteriosclerosis in a Humanized Mouse Aortic Xenograft Model

Abele-Ohl

Leis

Wollin

et al. 2012

American Journal of Transplantation

View full text Add to dashboard Cite

show abstract

Human cytomegalovirus infection in human renal arteries in vitro

Cited by 19 publications

References 21 publications

HCMV infection triggers smooth muscle cell proliferation in a 3D human coronary in vitro model

HCMV infection triggers smooth muscle cell proliferation in a 3D human coronary in vitro model

Modeling of Human Cytomegalovirus Maternal-Fetal Transmission in a Novel Decidual Organ Culture

Human Cytomegalovirus Infection Leads to Elevated Levels of Transplant Arteriosclerosis in a Humanized Mouse Aortic Xenograft Model

Contact Info

Product

Resources

About