Human Cytomegalovirus PUL97 Drug-Resistance Mutations in Congenitally Neonates and HIV-infected, No-Drug-Treated Patients

Mirarab, Azam; Mohebbi, Alireza; Javid, Naeme; Moradi, Abdolvahab; Vakili, Mohammad Ali; Tabarraei, Alijan

doi:10.2217/fvl-2016-0089

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…In a parallel work, we investigated pUL97 polymorphism in both groups [20] and found that there are significantly more polymorphisms in pUL97 from congenitally infected neonates than from HIV-positive adults. It seems both immune system pressure and viral interactions may be involved in pUL27 sequence variation.…”

Section: Discussionmentioning

confidence: 99%

Frequent pUL27 Variations in HIV-Infected Patients

Mirarab

Mohebbi²,

Moradi³

et al. 2016

Intervirology

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Objective: Drug-resistant isolates of human cytomegalovirus (HCMV) have led to the development of new anti-HCMV drugs. Maribavir (MBV) is a novel inhibitor of the HCMV viral kinase. Resistance to MBV is mapped to gene UL27, a viral nuclear protein. In this study, we investigated UL27 polymorphisms in MBV-naive HIV-positive and HCMV congenitally infected clinical samples. Methods: DNA was extracted from 20 CMV-positive HIV (9/20) and congenitally infected (11/20) patients and used for UL27 polymerase chain reaction amplification. Sanger sequencing and multiple sequence alignment of products was performed. Results: K90 was the most prevalent polymorphism in both HIV-positive and congenitally infected patients. Polymorphisms Q54, D123, and R107 (10%) were seen in more than one sample. There were significantly more polymorphisms in the HIV-positive samples (p = 0.038). Conclusion: HCMV pUL27 is highly variable in adult immunocompromised HIV-positive patients.

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Section: Discussionmentioning

confidence: 99%

Frequent pUL27 Variations in HIV-Infected Patients

Mirarab

Mohebbi²,

Moradi³

et al. 2016

Intervirology

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“…Amino acid substitutions were analyzed for their potential to confer any anti-HBV drug resistance or functional impact on the structural configuration by Mutation Assessor server as described before [16].…”

Section: Evaluation Of Drug-resistance Conferring Substitutions and Hmentioning

confidence: 99%

Hepatitis B virus reverse transcriptase polymorphisms between treated and treatment-naïve chronically infected patients

et al. 2019

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The aim of this study was investigation of variation(s) in the Hepatitis B virus (HBV) reverse transcriptase domain. 120 patients with chronic HBV infection recruited. 104 patients were received nucleos(t)ide analogs treatments. DNA extractions were done from plasma samples. Direct sequencing and alignment of Polymerase Chain Reaction products were applied for further analysis. HBV genotypes determined by NCBI's Genotyping Tool. Polymorphism(s) were detected by using DnaSP software. Of 120 samples, 98 were sequenced. All of products were HBV genotype D. 13/98 (13.27%) of patients had M539I/V substitutions corresponding to YMDD motif. FLLAQ to FLMAQ was observed among 22/98 (22.98) patients. Two substitutions N459Y and L515M were significantly correlated (R 2 = 0.486 and R 2 = 0.941 respectively) with FLLAQ motif variation. Mutation ratio among treatmentreceived patients to treatment-naïve patients was 0.2-0.6. Drug resistance conferring substitutions (DRCSs) were rtL180M (22/98), rtA194V (11/98), rtM204V (1/98), and rtM204I (11/98). Furthermore, six variants were observed among all patients. Appearance of DRCSs in HBV polymerase is a major obstacle to the virus treatments. In the present study, it was shown that DRCSs are more prevalent among treated patients. Therefore, replacement of current anti-viral regimen with novel anti-HBV drugs is warranted in the future.

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“…Human cytomegalovirus (HCMV), also known as human herpes virus-5, is a ubiquitous organism from the Herpesviridae family [1]. HCMV infection can affect multiple organs and is a main health concern in congenitally infected infants [2,3] and immunocompromised patients [4]. The infection is more prevalent among individuals at risk of human immunodeficiency virus (HIV) infection, drug abusers, and homosexual men infected with HIV [5].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Gastrointestinal Human Cytomegalovirus Infection in Biopsy Samples from Urease Positive Patients

Pourmomen

Mohebbi

Bagheri

et al. 2018

jcbr

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Introduction: Human cytomegalovirus (CMV) is a ubiquitous organism that can infect multiple organs but rarely causes gastrointestinal (GI) symptoms. However, it can be reactivated in the GI tract and lead to gastric cancer. Here, we investigated CMV infection in patients with gastric complaints. Material and methods: In this study, 99 biopsy samples were collected from patients with GI symptoms and CMV genome was detected by polymerase chain reaction. Sequencing was performed for computational genotyping. Results: We observed that 88.99% of the samples were urease positive and 5.99% of the samples were CMV positive. Moreover, genotype of the samples belonged to group 4 (gB4). There was a relatively high incidence of CMV infection in patients with GI problems. Conclusions: Although symptomatic CMVassociated diseases of the GI tract are rare in immunocompetent individuals, they might be considered as potential complications of CMV infection.

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Human Cytomegalovirus PUL97 Drug-Resistance Mutations in Congenitally Neonates and HIV-infected, No-Drug-Treated Patients

Cited by 8 publications

References 26 publications

Frequent pUL27 Variations in HIV-Infected Patients

Frequent pUL27 Variations in HIV-Infected Patients

Hepatitis B virus reverse transcriptase polymorphisms between treated and treatment-naïve chronically infected patients

Characterization of Gastrointestinal Human Cytomegalovirus Infection in Biopsy Samples from Urease Positive Patients

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