Human Dermal Fibroblast: A Promising Cellular Model to Study Biological Mechanisms of Major Depression and Antidepressant Drug Response

Mesdom, Pierre; Colle, Romain; Lebigot, Élise; Trabado, Séverine; Deflesselle, Éric; Fève, Bruno; Becquemont, Laurent; Verstuyft, Céline

doi:10.2174/1570159x17666191021141057

Cited by 14 publications

(8 citation statements)

References 204 publications

(207 reference statements)

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“…Initially, the cytotoxic effect of Pep1–Pep4 peptides on normal human dermal fibroblasts (HDFs) was investigated. NHDFs are mainly found in the dermis and play an important role in the production of the extracellular matrix (ECM) and wound healing ( Mesdom et al, 2020 ). The cells were treated with Pep1–Pep4 at a concentration of 200 µM for 24 h, and their cytotoxicity was determined using a resazurin reduction assay.…”

Section: Resultsmentioning

confidence: 99%

Characterization of four peptides from milk fermented with kombucha cultures and their metal complexes—in search of new biotherapeutics

Kamińska,

Hecel,

Słowik

et al. 2024

Front. Mol. Biosci.

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The most common skin diseases include eczema, psoriasis, acne, and fungal infections. There is often no effective cure for them. Increasing antimicrobial drug resistance prompts us to search for new, safe, and effective therapeutics. Among such interesting candidates are peptides derived from milk fermented with specific lactic acid bacteria or with kombucha cultures, which are a potential treasure trove of bioactive peptides. Four of them are discussed in this article. Their interactions with zinc and copper ions, which are known to improve the well-being of the skin, were characterized by potentiometry, MS, ITC, and spectroscopic methods, and their cytostatic potential was analyzed. The results suggest that they are safe for human cells and can be used alone or in complexes with copper for further testing as potential therapeutics for skin diseases.

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Section: Resultsmentioning

confidence: 99%

Characterization of four peptides from milk fermented with kombucha cultures and their metal complexes—in search of new biotherapeutics

Kamińska,

Hecel,

Słowik

et al. 2024

Front. Mol. Biosci.

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“…HDFs could be a convenient disease model without genetic engineering to study the disease mechanisms of several human diseases. HDFs have been used to study several neuropsychiatric disorders [ 22 , 23 ]. However, isolation and culture techniques in HDFs are time-consuming and require materials and extensive studies to improve the optimization of the protocols [ 24 ].…”

Section: Sacsin Functions and Cell/animal Models Of Arsacsmentioning

confidence: 99%

“…In bacteria, the HEPN region could impact antibiotic resistance (for example, kanamycin). In eukaryotes, the HEPN domain may have nucleotide-binding activity, but it could also bind anionic compounds in neurons [ 22 , 23 , 32 , 33 ]. HEPN domains could dimerize and form a high-affinity site for GTP binding, and potentially impact the chaperon activity of the sacsin protein.…”

Section: Sacsin Protein Domainsmentioning

confidence: 99%

Genetics of Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay (ARSACS) and Role of Sacsin in Neurodegeneration

Bagaria

Bagyinszky

2022

IJMS

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Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is an early-onset neurodegenerative disease that was originally discovered in the population from the Charlevoix-Saguenay-Lac-Saint-Jean (CSLSJ) region in Quebec. Although the disease progression of ARSACS may start in early childhood, cases with later onset have also been observed. Spasticity and ataxia could be common phenotypes, and retinal optic nerve hypermyelination is detected in the majority of patients. Other symptoms, such as pes cavus, ataxia and limb deformities, are also frequently observed in affected individuals. More than 200 mutations have been discovered in the SACS gene around the world. Besides French Canadians, SACS genetics have been extensively studied in Tunisia or Japan. Recently, emerging studies discovered SACS mutations in several other countries. SACS mutations could be associated with pathogenicity either in the homozygous or compound heterozygous stages. Sacsin has been confirmed to be involved in chaperon activities, controlling the microtubule balance or cell migration. Additionally, sacsin may also play a crucial role in regulating the mitochondrial functions. Through these mechanisms, it may share common mechanisms with other neurodegenerative diseases. Further studies are needed to define the exact functions of sacsin. This review introduces the genetic mutations discovered in the SACS gene and discusses its pathomechanisms and its possible involvement in other neurodegenerative diseases.

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“…As a neurotransmitter, neuropeptides are a group of short-chain amino acids. One of the initial interests in biological psychiatry was the role of modified endocrine function in mood disorders [ 86 ].…”

Section: Gpcrs and Mood Disordersmentioning

confidence: 99%

Insights into the Promising Prospect of G Protein and GPCR-Mediated Signaling in Neuropathophysiology and Its Therapeutic Regulation

Rahman

Islam

Mim

et al. 2022

Oxidative Medicine and Cellular Longevity

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G protein-coupled receptors (GPCRs) are intricately involved in the conversion of extracellular feedback to intracellular responses. These specialized receptors possess a crucial role in neurological and psychiatric disorders. Most nonsensory GPCRs are active in almost 90% of complex brain functions. At the time of receptor phosphorylation, a GPCR pathway is essentially activated through a G protein signaling mechanism via a G protein-coupled receptor kinase (GRK). Dopamine, an important neurotransmitter, is primarily involved in the pathophysiology of several CNS disorders; for instance, bipolar disorder, schizophrenia, Parkinson’s disease, and ADHD. Since dopamine, acetylcholine, and glutamate are potent neuropharmacological targets, dopamine itself has potential therapeutic effects in several CNS disorders. GPCRs essentially regulate brain functions by modulating downstream signaling pathways. GPR6, GPR52, and GPR8 are termed orphan GPCRs because they colocalize with dopamine D1 and D2 receptors in neurons of the basal ganglia, either alone or with both receptors. Among the orphan GPCRs, the GPR52 is recognized for being an effective psychiatric receptor. Various antipsychotics like aripiprazole and quetiapine mainly target GPCRs to exert their actions. One of the most important parts of signal transduction is the regulation of G protein signaling (RGS). These substances inhibit the activation of the G protein that initiates GPCR signaling. Developing a combination of RGS inhibitors with GPCR agonists may prove to have promising therapeutic potential. Indeed, several recent studies have suggested that GPCRs represent potentially valuable therapeutic targets for various psychiatric disorders. Molecular biology and genetically modified animal model studies recommend that these enriched GPCRs may also act as potential therapeutic psychoreceptors. Neurotransmitter and neuropeptide GPCR malfunction in the frontal cortex and limbic-related regions, including the hippocampus, hypothalamus, and brainstem, is likely responsible for the complex clinical picture that includes cognitive, perceptual, emotional, and motor symptoms. G protein and GPCR-mediated signaling play a critical role in developing new treatment options for mental health issues, and this study is aimed at offering a thorough picture of that involvement. For patients who are resistant to current therapies, the development of new drugs that target GPCR signaling cascades remains an interesting possibility. These discoveries might serve as a fresh foundation for the creation of creative methods for pharmacologically useful modulation of GPCR function.

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Human Dermal Fibroblast: A Promising Cellular Model to Study Biological Mechanisms of Major Depression and Antidepressant Drug Response

Cited by 14 publications

References 204 publications

Characterization of four peptides from milk fermented with kombucha cultures and their metal complexes—in search of new biotherapeutics

Characterization of four peptides from milk fermented with kombucha cultures and their metal complexes—in search of new biotherapeutics

Genetics of Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay (ARSACS) and Role of Sacsin in Neurodegeneration

Insights into the Promising Prospect of G Protein and GPCR-Mediated Signaling in Neuropathophysiology and Its Therapeutic Regulation

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