Human dilated ascending aorta: Mechanical characterization via uniaxial tensile tests

Ferrara, Antonella; Morganti, Simone; Totaro, Pasquale; Mazzola, Alessandro; Auricchio, Ferdinando

doi:10.1016/j.jmbbm.2015.08.021

Cited by 74 publications

(45 citation statements)

References 54 publications

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“…However, these results are still in the range of other blood vessels found in the human body, such as the great saphenous vein (ca. 7 MPa) (Han et al , ) or the aorta (between 18.34 ± 9.00 MPa for the anterior zone and 30.03 ± 13.85 MPa for the posterior zone in the circumferential direction) (Ferrara et al , ). Increase in modulus lead to an increase in UTS, which has a positive impact on the mechanical strength of the construct.…”

Section: Discussionmentioning

confidence: 99%

Microstructured human fibroblast-derived extracellular matrix scaffold for vascular media fabrication

Bourget

Laterreur

Gauvin

et al. 2016

J Tissue Eng Regen Med

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In the clinical and pharmacological fields, there is a need for the production of tissue-engineered small-diameter blood vessels. We have demonstrated previously that the extracellular matrix (ECM) produced by fibroblasts can be used as a scaffold to support three-dimensional (3D) growth of another cell type. Thus, a resistant tissue-engineered vascular media can be produced when such scaffolds are used to culture smooth muscle cells (SMCs). The present study was designed to develop an anisotropic fibroblastic ECM sheet that could replicate the physiological architecture of blood vessels after being assembled into a small diameter vascular conduit. Anisotropic ECM scaffolds were produced using human dermal fibroblasts, grown on a microfabricated substrate with a specific topography, which led to cell alignment and unidirectional ECM assembly. Following their devitalization, the scaffolds were seeded with SMCs. These cells elongated and migrated in a single direction, following a specific angle relative to the direction of the aligned fibroblastic ECM. Their resultant ECM stained for collagen I and III and elastin, and the cells expressed SMC differentiation markers. Seven days after SMCs seeding, the sheets were rolled around a mandrel to form a tissue-engineered vascular media. The resulting anisotropic ECM and cell alignment induced an increase in the mechanical strength and vascular reactivity in the circumferential direction as compared to unaligned constructs. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Microstructured human fibroblast-derived extracellular matrix scaffold for vascular media fabrication

Bourget

Laterreur

Gauvin

et al. 2016

J Tissue Eng Regen Med

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“…One of the major challenges in stress‐based risk evaluation is lack of tissue properties on a patient‐specific basis. There is a large body of literature pertaining to the in vitro mechanical properties of ATAA (see, eg, previous works. Together, these contributions shed significant light on understanding the behavior of this diseased tissue.…”

Section: Introductionmentioning

confidence: 94%

“…It was commonly reported that ATAAs exhibit a significant level of intersubject and intrasubject heterogeneities in their mechanical properties. The properties can vary regionally, and directionally . They can be affected by disease conditions such as Marfan syndrome and aortic valve phenotype .…”

Section: Introductionmentioning

confidence: 99%

Machine learning–aided exploration of relationship between strength and elastic properties in ascending thoracic aneurysm

Luo

Fan

Baek

et al. 2018

Numer Methods Biomed Eng

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Machine learning was applied to classify tension-strain curves harvested from inflation tests on ascending thoracic aneurysm samples. The curves were classified into rupture and nonrupture groups using prerupture response features. Two groups of features were used as the basis for classification. The first was the constitutive parameters fitted from the tension-strain data, and the second was geometric parameters extracted from the tension-strain curve. Based on the importance scores provided by the machine learning, implications of some features were interrogated. It was found that (1) the value of a constitutive parameter is nearly the same for all members in the rupture group and (2) the strength correlates strongly with a tension in the early phase of response as well as with the end stiffness. The study suggests that the strength, which is not available without rupturing the tissue, may be indirectly inferred from prerupture response features.

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“…Regarding the non-invasive ultrasound-based technology, the acquired data must be transferred into the readable mechanical data, and a reliable algorithm has to be established and verified, in this regard, it is not so convenient. In any case, the uniaxial tensile test as a simple and effective method, although it has some disadvantages, it is still widely employed to study the relevant mechanical issues including the anisotropy of arteries [7–9]. …”

Section: Introductionmentioning

confidence: 99%

Re-examination of the mechanical anisotropy of porcine thoracic aorta by uniaxial tensile tests

Chen

Wang

2016

BioMed Eng OnLine

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ObjectiveConsidering past studies on the orthotropic anisotropy of arteries in the circumferential and axial directions, this work aims to experimentally study the anisotropic behaviour of arteries by tensioning multi-directional strips of porcine thoracic aorta.MethodsHistology is first analyzed by staining arterial sections of three orthotropic (axial, circumferential, and radial) planes. 168 stripped samples from 21 aortas are categorized into three loading-rate groups to investigate the influence of loading rates on the Young’s modulus and ultimate stress. Basing on the Young’s modulus and ultimate stress, the degree of anisotropy is calculated. Moreover, 24 stripped samples from 3 aortas are tested to study the relaxation anisotropy of arteries by fitting the experimental data with a five-parameter Maxwell reduced relaxation function.ResultsHistological analysis shows the parallel orientation of crimpled collagen and elastin fibres. The Young’s modulus and ultimate stress reach the greatest in the circumferential direction, and the smallest in the axial direction, respectively, and the values in the other directions are in-between; moreover, the two parameters monotonously increase as the samples orientate from the axial to circumferential directions. The Young’s modulus is more sensitive to the loading rate than the ultimate stress. The degree of anisotropy calculated by the Young's modulus is similar to that by the ultimate stress, and it is independent of loading rates. Stress-relaxation also exhibits anisotropy, whose variation is consistent with those of the two parameters.ConclusionsDue to the stress-growth rule, fibre preferably orientates in the circumferential direction, and the preferable orientation results in great mechanical parameters, anisotropy, and small relaxation behaviour of arteries. The work extends the studies on the arterial anisotropy instead of only the circumferential and axial directions, and could be useful to comprehensively understand the anisotropy of arteries.

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Human dilated ascending aorta: Mechanical characterization via uniaxial tensile tests

Cited by 74 publications

References 54 publications

Microstructured human fibroblast-derived extracellular matrix scaffold for vascular media fabrication

Microstructured human fibroblast-derived extracellular matrix scaffold for vascular media fabrication

Machine learning–aided exploration of relationship between strength and elastic properties in ascending thoracic aneurysm

Re-examination of the mechanical anisotropy of porcine thoracic aorta by uniaxial tensile tests

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