2017
DOI: 10.3389/fmicb.2017.00019
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Human-Driven Microbiological Contamination of Benthic and Hyporheic Sediments of an Intermittent Peri-Urban River Assessed from MST and 16S rRNA Genetic Structure Analyses

Abstract: Rivers are often challenged by fecal contaminations. The barrier effect of sediments against fecal bacteria was investigated through the use of a microbial source tracking (MST) toolbox, and by Next Generation Sequencing (NGS) of V5-V6 16S rRNA gene (rrs) sequences. Non-metric multi-dimensional scaling analysis of V5-V6 16S rRNA gene sequences differentiated bacteriomes according to their compartment of origin i.e., surface water against benthic and hyporheic sediments. Classification of these reads showed the… Show more

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Cited by 27 publications
(20 citation statements)
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“…Therefore, WWO events represent huge delivery of faecal bacteria and pathogens in the river system. Other sources of faecal bacteria are also present in the catchment as indicated by Marti et al (2017), such as those coming from wild animals and livestocks (birds, cattle, horses, and goats), and organic soil amendments including composted manure in the rural areas.…”
Section: Methodsmentioning
confidence: 96%
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“…Therefore, WWO events represent huge delivery of faecal bacteria and pathogens in the river system. Other sources of faecal bacteria are also present in the catchment as indicated by Marti et al (2017), such as those coming from wild animals and livestocks (birds, cattle, horses, and goats), and organic soil amendments including composted manure in the rural areas.…”
Section: Methodsmentioning
confidence: 96%
“…The relative impact of WWO on the ecological state of small streams has been explored and shown to significantly modify river sediment microbiome organizations inferred from 16S rRNA polymerase chain reaction (PCR) gene products DNA sequences (Marti et al, 2017). These modifications were suggested to be linked to the nutrient inputs (organic‐C and N) discharged by CSOs.…”
Section: Introductionmentioning
confidence: 99%
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“…Communal wastewaters contribute to increased phosphorus levels in surface waters [8]. Phosphorus is rarely found Cyclic variations in TTC numbers in water are attributed to water influx due to rainfalls [9][10][11]. However, according to the results obtained during fall and winter, it is clear that lower temperatures do not significantly impact decrease in TTC numbers.…”
Section: Methodsmentioning
confidence: 98%