Human embryonic liver cell in culture A preliminary report

Imai, Hiromichi

doi:10.4044/joma1947.89.3-4_237

Cited by 2 publications

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been often observed that mass cultures from livers were rapidly dominated by fibroblast-like cells [6,9]. Various methods to separate epithelial-like cells from fibroblast-like cells have been described.…”

Section: Introductionmentioning

confidence: 99%

Selective growth of epithelial-like clear cells from adult rat liver by short-term exposure to glucocorticoids in primary culture

et al. 1982

View full text Add to dashboard Cite

Summary. Isolated liver cells, which were prepared from adult rats by a trypsin-liver-perfusion technique, were treated with dexamethasone or hydrocortisone at a concentration of 7.7 × 10 -6 M for 8 days in primary culture. The treated cultures displayed homogeneous population consisting of epithelial-like clear cells, while the untreated cultures displayed mixed population consisting of epithelial-like clear cells and fibroblast-like cells. The epithelial-like clear cells, which proliferated in the cultures treated with glucocorticoids for 8 days in primary culture, did not show any morphological changes following cultivation in glucocorticoid-free medium. After continuous glucocorticoid-treatment for more than 1 month, the treated cultures showed relatively low cell densities at confluence. The surface area of individual epithelial-like clear cells in the cultures treated with glucocorticoids for long periods of time was evidently greater than that in the cultures treated for only 8 days. The epithelial-like clear cells had glucose 6-phosphatase and tyrosine aminotransferase activities even though the levels of these enzymeactivities were very low compared with those in rat liver homogenates.

show abstract

Section: Introductionmentioning

confidence: 99%