Human Embryonic Stem Cell-Derived Oligodendrocytes: Protocols and Perspectives

Alsanie, Walaa F.; Niclis, Jonathan C.; Petratos, Steven

doi:10.1089/scd.2012.0520

Cited by 45 publications

(40 citation statements)

References 118 publications

(304 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differentiating hPSCs into OPCs or neural progenitor cells (NPCs) with a high purity has been demonstrated, but their function and maturation are still under investigation [12][13][14] . Extracellular matrix (ECM) plays an important role in neural differentiation of hPSCs and the maturation of the derived neural cells [15] .…”

Section: Introductionmentioning

confidence: 99%

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Liu

Yan

et al. 2014

WJSC

View full text Add to dashboard Cite

Neural cells differentiated from pluripotent stem cells (PSCs), including both embryonic stem cells and induced pluripotent stem cells, provide a powerful tool for drug screening, disease modeling and regenerative medicine. High-purity oligodendrocyte progenitor cells (OPCs) and neural progenitor cells (NPCs) have been derived from PSCs recently due to the advancements in understanding the developmental signaling pathways. Extracellular matrices (ECM) have been shown to play important roles in regulating the survival, proliferation, and differentiation of neural cells. To improve the function and maturation of the derived neural cells from PSCs, understanding the effects of ECM over the course of neural differentiation of PSCs is critical. During neural differentiation of PSCs, the cells are sensitive to the properties of natural or synthetic ECMs, including biochemical composition, biomechanical properties, and structural/topographical features. This review summarizes recent advances in neural differentiation of human PSCs into OPCs and NPCs, focusing on the role of ECM in modulating the composition and function of the differentiated cells. Especially, the importance of using three-dimensional ECM scaffolds to simulate the in vivo microenvironment for neural differentiation of PSCs is highlighted. Future perspectives including the immediate applications of PSC-derived neural cells in drug screening and disease modeling are also discussed.© 2014 Baishideng Publishing Group Co., Limited. All rights reserved.Key words: Pluripotent stem cells; Neural differentiation; Extracellular matrix; Three-dimensional; Drug screening Core tip: Neural cells derived from human pluripotent stem cells (hPSCs), including oligodendrocyte progenitor cells and neural progenitor cells, emerge as an unlimited and physiologically relevant cell source for drug screening, disease modeling, and regenerative medicine. Natural and synthetic extracellular matrices play an important role in regulating neural differentiation, cell migration, and the derived neural cell maturation. Recent advances in neural differentiation of hPSCs on extracellular matrices in 2-D and 3-D systems are reviewed in this paper. The immediate applications of the derived neural cells in drug screening and disease modeling are also discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Liu

Yan

et al. 2014

WJSC

View full text Add to dashboard Cite

show abstract

“…Human embryonic stem cells (hESCs) have proved a promising source for the generation and replacement of mature oligodendrocytes (95). Accordingly, hESC-derived oligodendrocytes can play a supportive role in the repair of CNS injuries (96). Intracerebroventricular transplanted hESC-derived oligodendroglial progenitor (hESC-OPs) cells ameliorated the clinical symptoms and promoted recovery from EA E paralysis.…”

Section: Embryonic Stem Cellsmentioning

confidence: 99%

Stem Cell Therapy: A Promising Therapeutic Approach for Multiple Sclerosis

Pourabdolhossein

Hamidabadi

Bojnordi

et al. 2017

Multiple Sclerosis: Perspectives in Treatment and Pathogenesis

View full text Add to dashboard Cite

Multiple sclerosis (MS) is an inflammatory disease of the central nervous system which is accompanied by demyelination of the nerves, axonal loss, and disability. Currently, no definitive treatment is recognized for MS. Stem-cell therapy for MS has shown promising results and has attracted attention as an alternative therapeutic option. Various stem cell sources such as mesenchymal, embryonic, and neural have been identified. This chapter gives an overview of the advances made in our understanding of these stem cells under two broad categories: exogenous and endogenous. Stem-cell therapy in MS and the substantial literature regarding their

show abstract

“…Various differentiation protocols have been developed to obtain oligodendrocytes and their progenitors from human embryonic stem cells (hESC) [95]. hESC-derived oligodendrocytes are susceptible to cell-culture derived JCPyV [96].…”

Section: Experimental Model Systems To Study Pyv Infectionmentioning

confidence: 99%

In Vitro and In Vivo Models for the Study of Human Polyomavirus Infection

Barth

Solis

Kack-Kack

et al. 2016

Viruses

View full text Add to dashboard Cite

Developments of genome amplification techniques have rapidly expanded the family of human polyomaviruses (PyV). Following infection early in life, PyV persist in their hosts and are generally of no clinical consequence. High-level replication of PyV can occur in patients under immunosuppressive or immunomodulatory therapy and causes severe clinical entities, such as progressive multifocal leukoencephalopathy, polyomavirus-associated nephropathy or Merkel cell carcinoma. The characterization of known and newly-discovered human PyV, their relationship to human health, and the mechanisms underlying pathogenesis remain to be elucidated. Here, we summarize the most widely-used in vitro and in vivo models to study the PyV-host interaction, pathogenesis and anti-viral drug screening. We discuss the strengths and limitations of the different models and the lessons learned.

show abstract

Human Embryonic Stem Cell-Derived Oligodendrocytes: Protocols and Perspectives

Cited by 45 publications

References 118 publications

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Stem Cell Therapy: A Promising Therapeutic Approach for Multiple Sclerosis

In Vitro and In Vivo Models for the Study of Human Polyomavirus Infection

Contact Info

Product

Resources

About