Human endothelial cell growth and coagulant function varies with respect to interfacial properties of polymeric substrates

Kottke‐Marchant, Kandice; Veenstra, Alexander A.; Marchant, Roger

doi:10.1002/(sici)1097-4636(199602)30:2<209::aid-jbm11>3.0.co;2-h

Cited by 52 publications

(26 citation statements)

References 48 publications

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“…We did not investigate other mediums minus fibronectin, because our cell culture protocol was developed using the FBS medium. During the design of the experiment we reviewed the literature and found that Kottke-Marchant et al, 17 in their study on fibronectin-coated polymers of different hydrophilicities, used FBS medium, which contained fibronectin.…”

Section: Discussionmentioning

confidence: 99%

“…14 The application of fibronectin, the cell adhesive protein, onto the surfaces of nonglycolide polymers has been utilized successfully in a number of studies on cell/substrate interactions. 15,16,17 Thus, we applied this technique to our scaffold samples. This attachment of cells to fibronectin-coated surfaces appears to be mediated via transmembrane ␤-1 integrin receptors present on the cell surfaces.…”

Section: Introductionmentioning

confidence: 99%

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The growth of chondrocytes into a fibronectin-coated biodegradable scaffold

Bhati

Mukherjee

McCarthy

et al. 2001

J. Biomed. Mater. Res.

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Porous scaffolds made from a biodegradable copolymer of trimethylene carbonate and glycolide were evaluated for tissue-engineered medical products. We examined the scaffold coated with cell adhesion protein and fibronectin and cultured under a dynamic mixing condition to enhance the growth of chondrocytes. Our hypothesis was that the combination of coating and dynamic mixing would be beneficial to the viability of the chondrocytic cells. Fibronectin was selected as the model protein because of its availability and routine assaying methods. Sterile samples of scaffolds of about 1 mm in thickness were coated with fibronectin at 37 degrees C for 1.5 h. Four groups of scaffolds were used: uncoated static or dynamic, and coated static or dynamic. Scaffold samples were placed in either a Petri dish or a spinner flask (static vs. dynamic groups) after inoculation with rat chondrocytes of an initial cell density of 1.29 x 10(5) cell/mL. After 7, 14, 21, and 28 days, each sample was fixed, embedded, and sectioned at 5 micro thickness. The sections were double-label immunostained using antibodies against cellular fibronectin synthesized by adherent cells as a measure of cell viability. A Hoechst 33258 nuclear stain was used to measure the number of cells attached to the scaffold at each time interval. The slides were examined using a fluorescence microscope to determine the cell ingrowth. At least 25 fields/treatment group (except the 7 day group) were measured. The data showed that cell in-growths into the porous scaffolds were higher at all time periods for the coated dynamic group than those for the other three groups.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The growth of chondrocytes into a fibronectin-coated biodegradable scaffold

Bhati

Mukherjee

McCarthy

et al. 2001

J. Biomed. Mater. Res.

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“…This seems to be rather a cell specific phenomenon, since other cell types were reported to spread on PDMS and other hydrophobic surfaces (Salloum et al 2005;Faid et al 2005;Kapur and Rudolph 1998;Toworfe et al 2004;Kottke-Marchant et al 1996). However, many observations suggest a general tendency of cells to prefer hydrophilic over hydrophobic surfaces by showing more rounded morphologies, corresponding to a lower degree of spreading, and lower seeding densities with increasing surface hydrophobicity (Schneider et al 2005;Altankov et al 1996;Kooten et al 1992;Kapur and Rudolph 1998;Kottke-Marchant et al 1996;Lampin et al 1997 and ref.…”

Section: Attachment Of Anchorage Dependent Cells To a Surface Ismentioning

confidence: 98%

Micropatterned surfaces of PDMS as growth templates for HEK 293 cells

Johann

Baiotto²,

Renaud

2007

Biomed Microdevices

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In this paper the easy and reliable preparation of precise micropatterns on PDMS surfaces is described and the growth of HEK 293 cells on those patterns during culture over several days is examined. The first patterning approach described is based on soft-lithography and polyelectrolyte multilayer deposition. Two different soft-lithographic techniques are employed for creating surface patterns of PAH, PSS, untreated and oxidized PDMS. The growth behavior of HEK 293 cells is investigated on all the dual combinations of the four surfaces, and decreasing preference of the cells for the surfaces in the order PAH (-NH2)>ox-PDMS (-OH)>>PSS (-SO3-)>PDMS (-CH3) is revealed. As the second patterning approach a method is introduced, which allows the deposition of gel droplets in a microarray format utilizing differences in the surface wettability. This concept is new and expected to be very useful for various applications. Finally, a speculative explanation for the different cell spreading behavior is provided considering the interplay between individual cell-surface interactions and a permanent cell tractional force.

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“…EC function has also been shown to depend on cell density and level of confluence [140]. A number of studies have noted significant differences in initial EC function dependent on the underlying substrate, which become insignificant when confluence is reached [140,144,164,165]. For example, bovine and porcine EC cultured sparsely on TCPS, coated with different concentrations of poly(HEMA) to vary adhesiveness, showed variations in F-actin and cell shape which correlated with levels of prostacyclin [166].…”

Section: Molecular Expression Studiesmentioning

confidence: 99%

The influence of biomaterials on endothelial cell thrombogenicity

2007

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Driven by tissue engineering and regenerative medicine, endothelial cells are being used in combination with biomaterials in a number of applications for the purpose of improving blood compatibility and host integration. Endothelialized vascular grafts are beginning to be used clinically with some success in some centers, while endothelial seeding is being explored as a means of creating a vasculature within engineered tissues. The underlying assumption of this strategy is that when cultured on artificial biomaterials, a confluent layer of endothelial cells maintain their nonthrombogenic phenotype. In this review the existing knowledge base of endothelial cell thrombogenicity cultured on a number of different biomaterials is summarized. The importance of selecting appropriate endpoint measures that are most reflective of overall surface thrombogenicity is the focus of this review. Endothelial cells inhibit thrombosis through three interconnected regulatory systems (1) the coagulation cascade (2) the cellular components of the blood such as leukocytes and platelets and (3) the complement cascade, and also through effects on fibrinolysis and vascular tone, the latter which influences blood flow. Thus, in order to demonstrate the thromobgenic benefit of seeding a biomaterial with EC, the conditions under which EC surfaces are more likely to exhibit lower thrombogenicity than unseeded biomaterial surfaces need to be consistent with the experimental context. The endpoints selected should be appropriate for the dominant thrombotic process that occurs under the given experimental conditions.

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Human endothelial cell growth and coagulant function varies with respect to interfacial properties of polymeric substrates

Cited by 52 publications

References 48 publications

The growth of chondrocytes into a fibronectin-coated biodegradable scaffold

The growth of chondrocytes into a fibronectin-coated biodegradable scaffold

Micropatterned surfaces of PDMS as growth templates for HEK 293 cells

The influence of biomaterials on endothelial cell thrombogenicity

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