Human endothelial cells produce orosomucoid,  an important component of the capillary barrier

Sörensson, Jenny; Matejka, Göran; Ohlson, Maria; Haraldsson, Börje

doi:10.1152/ajpheart.1999.276.2.h530

Cited by 79 publications

(77 citation statements)

References 25 publications

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“…Orosomucoid, a plasma glycoprotein, which is synthesized in the liver and may be also secreted by endothelial cells, 38 increases several folds during infection or trauma. 29,35 Orosomucoid is also bound to the surface of cultured bovine pulmonary microvascular endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

“…36 It has been found that orosomucoid modulates the transport of charged solutes across the wall of peripheral and cerebral microvessels in vivo by decreasing the transport of negatively charged molecules but increasing that of positively charged ones, 13,23,46 suggesting that orosomucoid plays an essential role in maintaining the permeability property of endothelium by adding negative charge to the SGL. 13,23,38 The second objective of the present study is thus to investigate whether orosomucoid modulates the bEnd3 monolayer permeability in a similar way as it does for the intact endothelium of the BBB.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Surface Charge of Immortalized Mouse Cerebral Endothelial Cell Monolayer on Transport of Charged Solutes

Yuan

2010

Ann Biomed Eng

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Abstract-Charge carried by the surface glycocalyx layer (SGL) of the cerebral endothelium has been shown to significantly modulate the permeability of the blood-brain barrier (BBB) to charged solutes in vivo. The cultured monolayer of bEnd3, an immortalized mouse cerebral endothelial cell line, is becoming a popular in vitro BBB model due to its easy growth and maintenance of many BBB characteristics over repeated passages. To test whether the SGL of bEnd3 monolayer carries similar charge as that in the intact BBB and quantify this charge, which can be characterized by the SGL thickness (L f ) and charge density (C mf ), we measured the solute permeability of bEnd3 monolayer to neutral solutes and to solutes with similar size but opposite charges: negatively charged a-lactalbumin (À11) and positively charged ribonuclease (+3). Combining the measured permeability data with a transport model across the cell monolayer, we predicted the L f and the C mf of bEnd3 monolayer, which is~160 nm and~25 mEq/L, respectively. We also investigated whether orosomucoid, a plasma glycoprotein modulating the charge of the intact BBB, alters the charge of bEnd3 monolayer. We found that 1 mg/mL orosomucoid would increase SGL charge density of bEnd3 monolayer to~2-fold of its control value.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Surface Charge of Immortalized Mouse Cerebral Endothelial Cell Monolayer on Transport of Charged Solutes

Yuan

2010

Ann Biomed Eng

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“…AGP is considered as a minor acute phase protein in cattle since its concentration increases only two-to four-fold during inflammation [30]. As the other acute phase proteins, AGP is produced mainly by hepatic cells, but local expression has been reported in human breast epithelial cells [11], stimulated alveolar macrophages [7] and human endothelial cells [29]. The detection of two of the major bovine APP, haptoglobin and serum amyloid A (SAA) in milk during mastitis [5] may suggest a role for APP also in the immunomodulation of the local inflammatory reaction in the udder.…”

Section: F Ceciliani Et Almentioning

confidence: 99%

Identification of the bovine α1-acid glycoprotein in colostrum and milk

et al. 2005

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-α1-acid glycoprotein (AGP) is an immunomodulatory protein expressed by hepatocytes in response to the systemic reaction that follows tissue damage caused by inflammation, infection or trauma. This paper presents the detection of bovine AGP (boAGP) in mammary secretions (colostrum and milk) and mammary gland tissue. Bovine AGP was detected by Western blotting in all the samples analysed, and could be quantified in colostrum at 162 (± 63.7) µg/mL and 114.5 (± 67.8) µg/mL during the first 12 h and 24 h respectively. In mature milk, the boAGP concentration clearly decreased and was no longer detectable using the Radial Immunodiffusion (RID) technique. The concentration of mature milk boAGP was therefore semi-quantified using an anion-exchange chromatographic procedure that allowed the concentration of the protein to be determined. The presence of AGP in bovine milk was confirmed by the internal sequence analysis performed following purification to homogeneity of the protein from milk. The concentration of AGP in bovine milk with low SCC (< 250 000) was very similar to that from bovine milk with high SCC (> 250 000). In order to investigate the origin of AGP in bovine milk, a search for mRNA was carried out in somatic cells and mammary gland tissue: mRNA expression of the boAGP gene was detected in mammary gland tissue, but not in somatic cells. Finally, the cDNA sequence of the boAGP was determined, and is hereby presented. α1-acid glycoprotein / mastitis / colostrum / milk

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“…Although extrahepatic production of this glycoprotein has also been described, its biological significance is still obscure (Gahmberg and Andersson, 1978;Sörensson et al, 1999;Fournier et al, 1999Fournier et al, , 2000.…”

Section: Introductionmentioning

confidence: 99%

A/G Gln20Arg (exon 1) and G/A Val156Met (exon 5) polymorphisms of the human orosomucoid 1 gene in Mexico

García-Ortiz

Vargas‐Alarcón²,

Jm³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. The human orosomucoid 1 gene (ORM1) codes an alpha-1-acid glycoprotein that has been classified as an acute-phase reactive protein, and a major drug-binding serum component, as well as an immunomodulatory protein with genetic polymorphisms. Evaluation of ORM variation through isoelectric focusing and immunobloting has revealed a world-wide distribution of the ORM1 F and ORM1 S alleles. We evaluated and examined the genetic characteristics of two Mexican populations that have different anthropo- logical and cultural antecedents, examining two ORM1 genotypes (exon 1 -A/G (Gln20Arg) and exon 5 G/A (Val156Met)) in 145 individuals, using nested polymerase chain reaction, sequencing, and restrited fragment length polymorphism. Mexican Mestizos had higher frequencies of the exon 1 A allele (P = 0.020) and AA genotype (P = 0.018) and lower frequency of the G allele (P = 0.020) when compared to Teenek Amerindians. When we examined exon 5 G/A (Val156Met) polymorphisms, we found significantly higher frequencies of the G allele (P = 0.0007) and the GG genotype (P = 0.0003) in the Mexican Mestizo population. The Teenek population had a significantly higher frequency of the A allele than has been reported for Chinese and African (P < 0.05) populations, and the G/A genotype was more frequently found in this Mexican population than in Chinese, African and European populations (P < 0.05).

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Human endothelial cells produce orosomucoid, an important component of the capillary barrier

Cited by 79 publications

References 25 publications

Effect of Surface Charge of Immortalized Mouse Cerebral Endothelial Cell Monolayer on Transport of Charged Solutes

Effect of Surface Charge of Immortalized Mouse Cerebral Endothelial Cell Monolayer on Transport of Charged Solutes

Identification of the bovine α1-acid glycoprotein in colostrum and milk

A/G Gln20Arg (exon 1) and G/A Val156Met (exon 5) polymorphisms of the human orosomucoid 1 gene in Mexico

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