Human Epithelial Teratocarcinoma Cells (P3): Radiobiological Characterization, DNA Damage, and Comparison with Other Rodent and Human Cell Lines

Hill, Colin K.; Holland, John J.; Chang-Liu, Chin Mei; Buess, E.; Peak, Jennifer G.; Peak, Meyrick J.

doi:10.2307/3577203

Cited by 24 publications

(14 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only one study is available concerning the induction of the medium transfer bystander eff ect and direct eff ect by low doses of neutrons (Liu et al 2006, and PhD thesis Liu, personal communication). It is well known that neutron radiation is signifi cantly more effi cient than gamma rays in killing cells and inducing chromosomal aberrations, gene mutations and oncogenic transformation (Field 1976, Hill et al 1988, Peak et al 1991. However, neutron radiation is always contaminated by a contribution from gamma radiation.…”

Section: Introductionmentioning

confidence: 99%

Neutrons do not produce a bystander effect in zebrafish irradiated in vivo

Wang

Smith

Duhig

et al. 2011

International Journal of Radiation Biology

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Neutrons do not produce a bystander effect in zebrafish irradiated in vivo

Wang

Smith

Duhig

et al. 2011

International Journal of Radiation Biology

View full text Add to dashboard Cite

show abstract

“…1987, Hill et al . 1988) by comparison with the elution of DNA from cells irradiated with 3 .0 Gy of 50-kVp X-rays .…”

Section: Strand Breakage Calculationsmentioning

confidence: 95%

“…The alkaline elution assay was performed as described by Kohn et al . (1981), procedure B, and by Hill et al . (1988) .…”

Section: Alkaline Elutionmentioning

confidence: 95%

“…In this paper we present information from studies in which we use alkaline elution techniques to measure the induction and repair of ssb produced by fission-spectrum neutrons in V79 Chinese hamster lung cells and P3 human epithelial teratocarcinoma cells . Sinclair and Fry (1987) stressed the importance of measuring responses to radiation in human cells, particularly epithelial cells, and the advantages of using P3 cells in molecular radiobiological research were described by Hill et al (1988) . We focus on the possibility that neutrons may cause a small component of highly lethal (unrepaired) lesions that behave as ssb under alkaline elution conditions .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

DNA Damage and Repair in Rodent and Human Cells after Exposure to JANUS Fission Spectrum Neutrons: A Minor Fraction of Single-strand Breaks as Revealed by Alkaline Elution is Refractory to Repair

Peak¹,

Peak²,

Carnes³

et al. 1989

International Journal of Radiation Biology

View full text Add to dashboard Cite

We have examined the induction and repair of breaks induced in the DNA of Chinese hamster V79 and human P3 epithelial teratocarcinoma cells by JANUS fission-spectrum neutrons (mean energy 0.85 MeV) and 60Co gamma radiation in the biological dose range, using alkaline filter elution methods. Fission-spectrum neutrons induce fewer immediate single-strand breaks (ssb) per gray of absorbed dose than do gamma rays, as measured by alkaline elution methods. Previous survival measurements have indicated incomplete recovery after neutron exposures. The present data demonstrate that whereas most ssb caused by exposure to fission-spectrum neutrons can be rapidly repaired by both cell lines, a small but statistically significant fraction of the ssb induced by exposure to 6 Gy of neutrons is refractory to repair. In contrast, all measurable ssb induced by 3 Gy gamma rays are rapidly repaired.

show abstract

“…The numbers of breaks induced in the DNA of human cells in culture by monochromatic 334nm radiation in the presence and absence of CPZ (200 pM) were calculated from the slopes of the elution profiles as described (Kohn et al, 1981) with an X-irradiated (3Gy) standard; 3 Gy of X-rays have been shown to induce 8.1 SSB per 1010 daltons in human cells (Hill et al, 1988).…”

mentioning

confidence: 99%

Chlorpromazine reduces UV-induced squamous cell carcinogenesis in hairless mice and enhances UV-induced DNA damage in cultured cells

Peak

Pfaff²,

Peraino³

1989

Br J Cancer

View full text Add to dashboard Cite

Summary Administration of the photoactivable compound chlorpromazine (CPZ) to SKH-1 hairless mice via their drinking water (CPZ, 0.01%) significantly reduced the rates of accumulation and yields of squamous cell carcinomas induced by long-term repeated exposures of these animals to solar UV radiation. This protective effect of CPZ was partially reversed in mice given a single injection of ethyl nitrosourea at birth. In in vitro studies, the presence of CPZ (0.2 mM) in mammalian cell cultures enhanced the yield of DNA singlestrand breaks induced in the cells by exposure to monochromatic UVA radiation at 334 nm. Collectively, the results suggest that CPZ may exert antineoplastic effects against UV-induced skin tumours by the induction of DNA damage.Chlorpromazine CPZ, 2-chloro-N,N-dimethyl-10-phenothiazine-10-propanamine), a commonly prescribed sedative, tranquiliser and anti-emetic, is also a highly photoactive compound (Kochevar, 1987). Photoactivation of CPZ produces oxidising CPZ radicals (including promazyl, peroxy and hydroxyl radicals) that are known to have cytotoxic effects on biological systems (Ciulla et al., 1986;Decuyper et al., 1986;Fujita et al., 1981). Therefore, the exposure of individuals ingesting CPZ to high fluences of ultraviolet A (UVA) radiation (radiation between 320nm and visible light, such as that found in solar UV or tanning booth radiation) should increase the body burden of oxidising radicals, especially in the epidermal tissues, into which the longer wavelengths of UVA readily penetrate (Bruls et al., 1984). This increment in tissue free radicals should enhance tumorigenesis, in keeping with the postulated role of free radicals as promoters of neoplasia (Gilbert, 1972;Greenstock & Ruddock, 1978;Greenstock & Wiebe, 1978;Pryor, 1978; Troll, 1978;Ames, 1983;Kinsella et al., 1983;Marx, 1983;Cerutti, 1985;Jones, 1985). Therefore, it might be anticipated that the ingestion of phenothiazines that are susceptible to photoactivation would enhance tumorigenic risk. Contrary to such expectations, however, clinical observations (in cancer patients given CPZ as a nausea suppressant during chemotherapy), epidemiological studies of mental hospital inmates receiving phenothiazine therapy and experimental evidence from studies with rodents indicate that phenothiazines such as CPZ may have antineoplastic activity (Jones, 1985;Darkin et al., 1984).Because none of the foregoing studies of phenothiazine antineoplasia addressed the issue of phenazine photoactivation and the biological effects of the resultant oxidising free radicals, we were prompted to perform an experiment to test possible enhanced or diminished neoplastic effects of dietary CPZ in groups of rodents exposed to carcinogenic solar UV radiations. The preliminary data have been reported previously (Peak et al., 1987b

show abstract

Human Epithelial Teratocarcinoma Cells (P3): Radiobiological Characterization, DNA Damage, and Comparison with Other Rodent and Human Cell Lines

Cited by 24 publications

References 24 publications

Neutrons do not produce a bystander effect in zebrafish irradiated in vivo

Neutrons do not produce a bystander effect in zebrafish irradiated in vivo

DNA Damage and Repair in Rodent and Human Cells after Exposure to JANUS Fission Spectrum Neutrons: A Minor Fraction of Single-strand Breaks as Revealed by Alkaline Elution is Refractory to Repair

Chlorpromazine reduces UV-induced squamous cell carcinogenesis in hairless mice and enhances UV-induced DNA damage in cultured cells

Contact Info

Product

Resources

About