Human ex vivo dentin-pulp complex preservation in a full crown model

Botelho, João; Cavacas, Maria Alzira; Borrecho, Gonçalo; Polido, Mário; Oliveira, Pedro; Santos, Jorge Costa

doi:10.1016/j.jobcr.2016.12.002

Cited by 4 publications

(1 citation statement)

References 26 publications

(26 reference statements)

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“…Nevertheless, such in vitro models are far from reality and they do not guarantee adequate translation in clinical practice. The necessity of studies closer to real conditions led to the development of ingenious ex vivo models such as dentin-pulp slices [ 7 ], the entire tooth or crown culture [ 8 , 9 ], and the mandible slice culture [ 10 ] to study pulp capping procedures, pulpitis [ 11 , 12 ] or scaffolds for pulp regeneration [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

In vitro, ex vivo, and in vivo models for dental pulp regeneration

Piglionico

Pons

Romieu

et al. 2023

J Mater Sci: Mater Med

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Based on the concept of tissue engineering (Cells—Scaffold—Bioactive molecules), regenerative endodontics appeared as a new notion for dental endodontic treatment. Its approaches aim to preserve dental pulp vitality (pulp capping) or to regenerate a vascularized pulp-like tissue inside necrotic root canals by cell homing. To improve the methods of tissue engineering for pulp regeneration, numerous studies using in vitro, ex vivo, and in vivo models have been performed. This review explores the evolution of laboratory models used in such studies and classifies them according to different criteria. It starts from the initial two–dimensional in vitro models that allowed characterization of stem cell behavior, through 3D culture matrices combined with dental tissue and finally arrives at the more challenging ex vivo and in vivo models. The travel which follows the elaboration of such models reveals the difficulty in establishing reproducible laboratory models for dental pulp regeneration. The development of well-established protocols and new laboratory ex vivo and in vivo models in the field of pulp regeneration would lead to consistent results, reduction of animal experimentation, and facilitation of the translation to clinical practice. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

In vitro, ex vivo, and in vivo models for dental pulp regeneration

Piglionico

Pons

Romieu

et al. 2023

J Mater Sci: Mater Med

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Adoption of Biomedical Ceramic iRoot BP in the Treatment of Localized Pulpitis in Children

Peng

Feng

Tan

2022

j biomater tissue eng

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To explore the clinical efficacy of biomedical ceramic iRoot BP in the treatment of localized acute pulpitis in children, and the effect of iRoot BP on proliferation and osteogenic differentiation of human dental pulp stem cells (hDPSCs), 72 localized acute pulpitis children admitted to our hospital from September 2018 to September 2019 were selected and divided into group A (treated with MTA pulp capping material) and group B (treated with iRoot BP material), and the clinical effect, pain degree, and adverse reactions (ADR) rate were compared. The effects of iRoot BP on hDPSCs proliferation and osteogenic differentiation were analyzed; the proliferative activity of cells in iRoot BP group, MTA group, and control group (C group) were measured by cholecystokinin-8 (CCK-8) assay, the ability of cell mineralized nodular formation was observed via alizarin red staining; and quantitative reverse transcription PCR (qRT-PCR) andWestern blot were adopted to determine the expression of osteogenic related genes of hDPSCs and key proteins of mitogen-activated protein kinase (MAPK) signaling pathway. After 1 week of treatment, the clinical efficacy of group B was more favorable in contrast with group A (P < 0.05); the pain of children in group B was notably better in contrast with group A, and incidence of ADR was notably lower in contrast with group A (P < 0.05). 5.0 mg/mL, 10.0 mg/mL, and 30 mg/mL iRoot BP or MTA could improve cell proliferation activity (P < 0.01); the effect of iRoot BP on proliferation of hDPSCs was greater in contrast with MTA (P < 0.05); and the integral optical density (IOD) value of iRoot BP group was notably higher in contrast with MTA group (P < 0.01). The mRNA expression levels of collagen-I (COL-I), bone sialoprotein (BSP), and osteocalcin (OC) in MTA group and iRoot BP group were notably higher in contrast with C group (P < 0.01); the COL-I mRNA expression level of iRoot BP group was notably higher in contrast with MTA group (P < 0.01); the mRNA expression level of BSP in MTA group was notably higher in contrast with iRoot BP group (P < 0.01); the relative protein expression levels of phosphorylated ERK (p-ERK) and phospho-Jun N-terminal kinase (p-JNK) in MTA group and iRoot BP group were notably higher in contrast with C group (P < 0.01); and the relative expression level of p-ERK protein in iRoot BP group was higher in contrast with MTA group (P < 0.05). These results indicated that the clinical efficacy of biomedical ceramic iRoot BP was better than MTA in the preservation of live pulpitis in children, and the patients treated with iRoot BP had better pain recovery effect and lower risk of ADR. The effect of iRoot BP on the proliferation and mineralization of hDPSCs was better than that of MTA, and it may promote the osteogenic differentiation of hDPSCs by activating MAPK signaling pathway and regulating gene expression of COL-I, BSP, and OC.

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Universalization of the intelligence definition problem

A.I.¹,

A.V.²

2021

Stuc.intelekt

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The main problem today in the research and development of AI is the lack of a scientific definition of Intelligence, since it is impossible to do something incomprehensible. This fundamentally delegitimizes all developments in this area and science as a whole as a product of exclusively intellectual activity, and any scientific use of the term «Intelligence» in its strict sense is unreasonable. In this paper, this problem is solved by transition to a more general universal paradigm of cognition, which allowed us to deduce the desired definition and universal formalism of Intelligence in its strong sense. Unlike previous publications, the ontology and properties of Intelligence are specified here as necessary components of Intelligence, which are subject to subsequent concretization and materialization in different niches of existence. The results of the work are of both fundamental and applied general scientific importance for all technical and humanitarian applications of Intelligence

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Human ex vivo dentin-pulp complex preservation in a full crown model

Cited by 4 publications

References 26 publications

In vitro, ex vivo, and in vivo models for dental pulp regeneration

In vitro, ex vivo, and in vivo models for dental pulp regeneration

Adoption of Biomedical Ceramic iRoot BP in the Treatment of Localized Pulpitis in Children

Universalization of the intelligence definition problem

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