Human Fallopian tubal epithelial cells in vitro:establishment of polarity and potential role of intracellular calcium and extracellular ATP in fluid secretion

Dickens, C. J.; Comer, M.T.; Southgate, Jennifer; Leese, Henry J.

doi:10.1093/oxfordjournals.humrep.a019021

Cited by 31 publications

(20 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of particular relevance to the present report, ATP has been reported transiently to stimulate I sc or V te across cultures of bovine, mouse, and human oviductal epithelium (8,9,19,21), and we have earlier shown that ATP stimulates I sc across cultures of monkey oviductal epithelium with not only a transient but also a sustained response (26). Conversion of CO 2 to HCO 3 Ϫ by carbonic anhydrase constitutes an important step in HCO 3 Ϫ secretion across many epithelia (6,15,16,31), and we therefore tested the effects of acetazolamide on the response to ATP.…”

Section: Discussionmentioning

confidence: 65%

Hormonal and purinergic stimulation of bicarbonate secretion in oviducts of rhesus monkey

Rajagopal

Fischer²,

Widdicombe

2008

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

cause an increase in the HCO 3 Ϫ concentration of oviductal liquid at midcycle is believed to markedly enhance fertility, we have studied active secretion of HCO 3 Ϫ across highly differentiated cultures of monkey oviductal epithelium. Cultured cell sheets were mounted in Ussing chambers and bathed in medium containing 25 mM HCO 3 Ϫ . Purinergic agents potently stimulated short-circuit current (Isc) with an initial transient response declining within ϳ2 min to a sustained response. The potency sequence of ATP Ϸ UTP Ͼ ADP ϾϾ AMP suggested that the I sc response was mediated mainly by P2Y2 receptors. Acetazolamide, an inhibitor of carbonic anhydrase, had little or no effect on baseline I sc or the transient response to ATP but abolished the sustained response to ATP. Similar results were obtained on sheets of native epithelium. In pH-stat experiments, the abluminal medium of cell cultures was bathed in HCO 3 Ϫ -CO2 medium, and the pH of the unbuffered luminal medium was maintained at ϳ7.4 by addition of strong acid or base. ATP stimulated base secretion, and this was inhibited by acetazolamide. Furthermore, these changes in secretion of base were in good quantitative agreement with the I sc responses. When phenol red (an estrogen) was removed from the culture medium, ATP-dependent HCO 3 Ϫ secretion was markedly reduced but could be restored by treatment with estradiol. Estrogens also markedly increased ciliation of the cultures. These results suggest that the midcycle increase in the HCO 3 Ϫ concentration of oviductal liquid may be mediated by the effects of estradiol on purinergic pathways or on ATP secretion. carbonic anhydrase; short-circuit current; estradiol; adenosine 5Ј-triphosphate THE OVIDUCTAL EPITHELIUM regulates the composition of the oviductal liquid, thereby creating a favorable environment for key reproductive events to take place. Among the most important components of oviductal liquid is HCO 3 Ϫ , which has been shown to promote oocyte maturation, sperm capacitation, fertilization, and zygote development (2). In fact, defective HCO 3 Ϫ transport might cause reduced fertility in women with cystic fibrosis (35).In vivo studies in many different species have shown that the bicarbonate concentration of oviductal liquid peaks in midcycle at approximately the same time as ovulation and a peak in plasma estrogen levels (13,20,23 Ϫ is by the same mechanism as that proposed for several epithelia, including those of the pancreas and airways (30,31). This classical mechanism has HCO 3 Ϫ entering the cell from the interstitium via a Na ϩ :HCO 3 Ϫ cotransporter or generated from CO 2 by carbonic anhydrase. The bicarbonate ions then exit via the apical membrane through a Cl Ϫ :HCO 3 Ϫ exchanger or anion channels such as the cystic fibrosis transmembrane conductance regulator (CFTR). Anion channels also function to recycle the Cl Ϫ entering the cell by the Cl Ϫ :HCO 3 Ϫ transporter (31). However, further analysis of either cellular mechanisms or of the neurohumoral regulation of HCO 3 Ϫ secretion by oviductal ep...

show abstract

Section: Discussionmentioning

confidence: 65%

Hormonal and purinergic stimulation of bicarbonate secretion in oviducts of rhesus monkey

Rajagopal

Fischer²,

Widdicombe

2008

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

show abstract

“…Bovine oviducts at various stages of the estrous cycle were removed from cattle at a local abattoir within 5 min of slaughter. Epithelial cells were isolated according to the methods previously described for the isolation of rat uterine epithelium (15,18). The oviducts were immediately separated from connective tissue, fat, and major blood vessels, and the fimbriae were removed.…”

Section: Methodsmentioning

confidence: 99%

“…The resulting luminal accumulation of sodium chloride generates an osmotic gradient pulling water into the lumen. In the oviduct, fluid secretion has been shown to be directly associated with chloride secretion (14,15). Oviduct fluid formation is essential to hydrate and protect the mucosal surface and to provide an appropriate ionic environment to facilitate healthy reproductive processes (25).…”

mentioning

confidence: 99%

Small conductance potassium channels drive ATP-activated chloride secretion in the oviduct

Keating

Quinlan

2012

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Keating N, Quinlan LR. Small conductance potassium channels drive ATP-activated chloride secretion in the oviduct. Am J Physiol Cell Physiol 302: C100 -C109, 2012. First published September 14, 2011; doi:10.1152/ajpcell.00503.2010.-The molecular mechanisms controlling fluid secretion within the oviduct have yet to be determined. As in other epithelia, both secretory and absorptive pathways are likely to work in tandem to drive appropriate ionic movement to support fluid movement across the oviduct epithelium. This study explored the role of potassium channels in basolateral extracellular ATP (ATP e)-stimulated ion transport in bovine oviduct epithelium using the Ussing chamber short-circuit current (I SC) technique. Basal I SC in bovine oviduct epithelium comprises both chloride secretion and sodium absorption and was inhibited by treatment with basolateral K ϩ channel inhibitors tetrapentlyammonium chloride (TPeA) or BaCl 2. Similarly, ATP-stimulated chloride secretion was significantly attenuated by pretreatment with BaCl 2, tetraethylammonium (TEA), tolbutamide, and TPeA. Basolateral K ϩ current, isolated using nystatin-perforation technique, was rapidly activated by ATP e, and pretreatment of monolayers with thapsigargin or TPeA abolished this ATP-stimulated K ϩ current. To further investigate the type of K ϩ channel involved in the ATP response in the bovine oviduct, a number of specific Ca 2ϩ -activated K ϩ channel inhibitors were tested on the ATP-induced ⌬I SC in intact monolayers. Charbydotoxin, (high conductance and intermediate conductance inhibitor), or paxilline, (high conductance inhibitor) did not significantly alter the ATP e response. However, pretreatment with the small conductance inhibitor apamin resulted in a 60% reduction in the response to ATP e. The presence of small conductance family member KCNN3 was confirmed by RT-PCR and immunohistochemistry. Measurements of intracellular calcium using Fura-2 spectrofluorescence imaging revealed the ability of ATP e to increase intracellular calcium in a phospholipase C-inositol 1,4,5-trisphosphate pathway-sensitive manner. In conclusion, these results provide strong evidence that purinergic activation of a calciumdependent, apamin-sensitive potassium conductance is essential to promote chloride secretion and thus fluid formation in the oviduct. oviduct epithelium; apamin; fertilization THE FLUID formed within the oviduct provides an essential and appropriate environment for gamete maturation, transport, fertilization, and early embryo development. Although the composition of this fluid has been well documented for some time (8,40), the mechanisms underlying fluid formation surprisingly remain poorly understood.As a general principle, fluid formation is secondary to and driven by the active transport of ions across an epithelial layer.

show abstract

“…The effect was mediated by activation of P2Y 2 or possibly P2Y 4 receptors (Cox and Leese, 1994;Villalon et al, 1995) located on the basal surface of the cells; it involved a rapid increase in Ca 2+ i due to Ca 2+ mobilization (Cox and Leese, 1994;Villalon et al, 1995;Dickens et al, 1996;Anon. ;Squires et al, 1995;Tiemann et al, 1996) and activation of Ca 2+ -dependent, apaminsensitive K + conductance (Keating and Quinlan, 2012).…”

Section: Fallopian Tubementioning

confidence: 99%

Purinergic Signalling in the Reproductive System

Gorodeski¹

2015

Autonomic Neuroscience

View full text Add to dashboard Cite

Human Fallopian tubal epithelial cells in vitro:establishment of polarity and potential role of intracellular calcium and extracellular ATP in fluid secretion

Cited by 31 publications

References 18 publications

Hormonal and purinergic stimulation of bicarbonate secretion in oviducts of rhesus monkey

Hormonal and purinergic stimulation of bicarbonate secretion in oviducts of rhesus monkey

Small conductance potassium channels drive ATP-activated chloride secretion in the oviduct

Purinergic Signalling in the Reproductive System

Contact Info

Product

Resources

About