2023
DOI: 10.1101/2023.02.04.527148
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Human FAM111A inhibits vaccinia virus replication by degrading viral DNA-binding protein I3 and is antagonized by poxvirus host range factor SPI-1

Abstract: Poxviruses are large double-stranded DNA viruses that infect a wide range of animals including humans. Smallpox virus, the most notorious poxvirus, was eradicated globally in the 1970s. Since then, other members of the poxvirus family, such as monkeypox virus (MPXV) are still posing a great threat to public health. Vaccinia virus (VACV) is a prototypic poxvirus and was used as the vaccine strain for smallpox eradication. VACV encodes a serine protease inhibitor 1 (SPI-1) conserved in all orthopoxviruses, which… Show more

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Cited by 2 publications
(5 citation statements)
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“…OPG208 (or SPI-1) is a key host range factor that enables replication in human cells, which has been proven in loss of function, gain of function and related experiments. 34,35 One remaining key question is how the variations mentioned above affect the MPXV transmissibility, pathogenicity, and adaption to the human host via functional studies and re-annotation.…”
Section: Discussionmentioning
confidence: 99%
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“…OPG208 (or SPI-1) is a key host range factor that enables replication in human cells, which has been proven in loss of function, gain of function and related experiments. 34,35 One remaining key question is how the variations mentioned above affect the MPXV transmissibility, pathogenicity, and adaption to the human host via functional studies and re-annotation.…”
Section: Discussionmentioning
confidence: 99%
“…In contrast to this, Monzón et al and this study have found 53+ copies for recent genomes. OPG208 (or SPI‐1) is a key host range factor that enables replication in human cells, which has been proven in loss of function, gain of function and related experiments 34,35 …”
Section: Discussionmentioning
confidence: 99%
“…These results suggested that SNAP29 is a novel host restriction factor for MVA, and is antagonized by virus A52. In recent years, two host restriction factors for MVA have been identified, namely zinc-finger antiviral protein (ZAP) and FAM111A, which could be antagonized by VACV proteins C16 and C12, respectively 9, 10 . Our discoveries added SNAP29 to this list and contributed fresh insights into comprehensive elucidation of the molecular mechanism underlying MVA’s inability to replicate efficiently in most mammalian cells.…”
Section: Discussionmentioning
confidence: 99%
“…Genes from VACV-WR, MPXV and LSDV were codon-optimized and cloned into the pcDNA3.1 vector. Ub-HA plasmid was kindly provided by Junda Zhu and was previously described 10 .…”
Section: Methodsmentioning
confidence: 99%
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