Human Fecal Microbiome–Based Biomarkers for Colorectal Cancer

Narayanan, Vilvapathy; Peppelenbosch, Maikel P.; Konstantinov, Sergey R.

doi:10.1158/1940-6207.capr-14-0273

Cited by 35 publications

(31 citation statements)

References 25 publications

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“…It is of interest since few or no report has been observed in literature. Interestingly, blood sample was obtained before colonoscopy and it is suggested that these microorganisms were able to achieve bloodstream through tumor tissue and they may remain in state of bacteremia for long period [6]. In this case, the infection in rectal cancer involves disruption of the normal barrier due to tumor-induced ulceration, followed by bloodstream invasion, in accordance with Mirza [7] Bacteremia caused by anaerobic bacteria represents from 0.5% to 12% of the total of bacteremia, and the mortality is approximately between 25% and 44% [8].…”

Section: Discussionmentioning

confidence: 99%

Bloodstream Infection due to Bacterial Co-Infection in Patients with Rectal Carcinoma: Report of Two Cases

Solis-Castro¹,

Alberca²,

Freire³

et al. 2017

Colorec Cancer

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In this study, we reported the bacterial co-infection causing bacteremia in two of 38 patients diagnosed with rectal carcinoma. 10 mL of blood of each patient were cultured in BHI broth. From positive blood cultures, aliquots were streaked on MacConkey agar (aerobiosis), and Bacteroides-bile-esculin agar and kanamycinblood agar (anaerobiosis). Bacteria were identified by using API-20A kit and confirmed by 16s rDNA sequencing. The bacterial identification showed the presence of Escherichia coli, Bacteroides fragilis and Enterococcus faecalis. The use of DNA sequencing showed to be a tool for rapid screening of blood infections than the classic microbiological methods, and provides an accurate and effective microbial identification in sepsis process. Patients with intestinal malignancy can produce bacteremia with nonspecific or atypical symptoms.

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Section: Discussionmentioning

confidence: 99%

Bloodstream Infection due to Bacterial Co-Infection in Patients with Rectal Carcinoma: Report of Two Cases

Solis-Castro¹,

Alberca²,

Freire³

et al. 2017

Colorec Cancer

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“…The best know example is the role Helicobater pylori plays in gastric cancer [43][44][45]. However, a handful of laboratories have reported links between bacterial infection and oral [11], colon [46][47][48][49], pancreatic [46,50], liver [51], esophageal [52] and prostate cancers [53]. The biological mechanism of these associations is not yet understood [54,55], however, there are several mechanisms by which bacterial infection could lead to the initiation and progression of oncogenic processes [42,56,57].…”

Section: Discussionmentioning

confidence: 99%

16S rRNA amplicon sequencing identifies microbiota associated with oral cancer, Human Papilloma Virus infection and surgical treatment

et al. 2016

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Systemic inflammatory events and localized disease, mediated by the microbiome, may be measured in saliva as head and neck squamous cell carcinoma (HNSCC) diagnostic and prognostic biomonitors. We used a 16S rRNA V3-V5 marker gene approach to compare the saliva microbiome in DNA isolated from Oropharyngeal (OPSCC), Oral Cavity Squamous Cell Carcinoma (OCSCC) patients and normal epithelium controls, to characterize the HNSCC saliva microbiota and examine their abundance before and after surgical resection.The analyses identified a predominance of Firmicutes, Proteobacteria and Bacteroidetes, with less frequent presence of Actinobacteria and Fusobacteria before surgery. At lower taxonomic levels, the most abundant genera were Streptococcus, Prevotella, Haemophilus, Lactobacillus and Veillonella, with lower numbers of Citrobacter and Neisseraceae genus Kingella. HNSCC patients had a significant loss in richness and diversity of microbiota species (p<0.05) compared to the controls. Overall, the Operational Taxonomic Units network shows that the relative abundance of OTU's within genus Streptococcus, Dialister, and Veillonella can be used to discriminate tumor from control samples (p<0.05). Tumor samples lost Neisseria, Aggregatibacter (Proteobacteria), Haemophillus (Firmicutes) and Leptotrichia (Fusobacteria). Paired taxa within family Enterobacteriaceae, together with genus Oribacterium, distinguish OCSCC samples from OPSCC and normal samples (p<0.05). Similarly, only HPV positive samples have an abundance of genus Gemellaceae and Leuconostoc (p<0.05). Longitudinal analyses of samples taken before and after Research PaperOncotarget 51321 www.impactjournals.com/oncotarget surgery, revealed a reduction in the alpha diversity measure after surgery, together with an increase of this measure in patients that recurred (p<0.05). These results suggest that microbiota may be used as HNSCC diagnostic and prognostic biomonitors.

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“…However, oncobiome screening could potentially be designed to detect not only individual bacterial species associated with cancer but also dysbiosis long before adenomatous polyps or cancer have developed. Indeed a recent study characterized the microbiota from ‘healthy’ subjects and those with adenomas or CRC as confirmed by colonoscopy [51,131], and found that, when combined with known clinical risk factors for CRC (age, race, body mass index), combining six specific operational taxonomic units (OTU) of gut microbiota in stool samples significantly increased the ability to differentiate healthy subjects from those with adenomas or CRC. Likewise, taxonomic markers were identified through metagenomic sequencing of fecal samples to distinguish CRC patients from tumor-free patients [50].…”

Section: Have We Reached the Mirage?mentioning

confidence: 99%

The Microbiome and Cancer: Is the ‘Oncobiome’ Mirage Real?

Thomas

Jobin

2015

Trends in Cancer

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Investigations focused on the interplay between the human microbiome and cancer development, herein termed the ‘oncobiome’, have been growing at a rapid rate. However, these studies to date have primarily demonstrated associative relationships rather than causative ones. We pose the question of whether this emerging field of research is a ‘mirage’ without a clear picture, or truly represents a paradigm shift for cancer research. We propose the necessary steps needed to answer crucial questions and push the field forward to bring the mirage into a tangible reality.

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Human Fecal Microbiome–Based Biomarkers for Colorectal Cancer

Cited by 35 publications

References 25 publications

Bloodstream Infection due to Bacterial Co-Infection in Patients with Rectal Carcinoma: Report of Two Cases

Bloodstream Infection due to Bacterial Co-Infection in Patients with Rectal Carcinoma: Report of Two Cases

16S rRNA amplicon sequencing identifies microbiota associated with oral cancer, Human Papilloma Virus infection and surgical treatment

The Microbiome and Cancer: Is the ‘Oncobiome’ Mirage Real?

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